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| Velini,Edivaldo Domingues; Trindade,Maria Lúcia Bueno; Alves,Elza; Catâneo,Ana Catarina; Marino,Celso Luis; Maia,Ivan de Godoy; Mori,Edson Seizo; Furtado,Edson Luiz; Guerrini,Iraê Amaral; Wilcken,Carlos Frederico. |
| Herbicides inhibit enzymatic systems of plants. Acetolactate synthase (ALS, EC = 4.1.3.18) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS, EC 2.5.1.19) are key enzymes for herbicide action. Hundreds of compounds inhibit ALS. This enzyme is highly variable, enabling the selective control of weeds in a number of crops. Glyphosate, the only commercial herbicide inhibiting EPSPS is widely used for non-selective control of weeds in many crops. Recently, transgenic crops resistant to glyphosate were developed and have been used by farmers. The aim of this study was the data mining of eucalypt expressed sequence tags (ESTs) in the FORESTs Genome Project database (<A HREF="https://forests.esalq.usp.br">https://forests.esalq.usp.br</A>) related... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eucalyptus; Herbicides; ALS; EPSPS. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400012 |
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| Velini,Edivaldo Domingues; Trindade,Maria Lúcia Bueno; Alves,Elza; Catâneo,Ana Catarina; Marino,Celso Luis; Maia,Ivan de Godoy; Mori,Edson Seizo; Furtado,Edson Luiz; Guerrini,Iraê Amaral; Wilcken,Carlos Frederico. |
| This work was aimed at locating Eucalyptus ESTs corresponding to the GS enzyme (Glutamine Synthetase, EC = 6.3.1.2) and to the D1 protein, which are directly related to resistance to herbicides that promote oxidative stress. Glutamine Synthetase corresponds to the site of action of the herbicide glufosinate. Herbicides that belong to groups such as ureas, uracils, triazines and triazinones act on the D1-Qb complex (receptor of electrons from the Photosystem II) by inactivating it. The clusters EGEQRT3302E01.g, EGEQRT3001F12.b; EGEZLV1203B04.g; EGBGFB1211H06.g and EGEZLV1205F09.g enclosed complete sequences (with 356 amino acids) of the Glutamine Synthetase enzyme. The cluster EGEQSL1054G06.g is a consensus of four reads and enclosed a complete sequence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eucalyptus; Glutamine synthetase; D1 protein; Herbicide. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400010 |
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| Velini,Edivaldo Domingues; Trindade,Maria Lúcia Bueno; Alves,Elza; Catâneo,Ana Catarina; Marino,Celso Luis; Maia,Ivan de Godoy; Mori,Edson Seizo; Furtado,Edson Luiz; Guerrini,Iraê Amaral; Wilcken,Carlos Frederico. |
| This work was aimed at locating Eucalyptus ESTs corresponding to the PROTOX or PPO enzyme (Protoporphyrinogen IX oxidase, E.C. 1.3.3.4) directly related to resistance to herbicides that promote oxidative stress, changing the functionality of this enzyme. PROTOX, which is the site of action of diphenyl-ether (oxyfluorfen, lactofen, fomesafen), oxadiazole (oxadiazon and oxadiargyl), and aryl triazolinone (sulfentrazone and carfentrazone) herbicides, acts on the synthesis route of porphyrins which is associated with the production of chlorophyll a, catalases, and peroxidases. One cluster and one single read were located, with e-values better than e-70, associated to PROTOX. The alignment results between amino acid sequences indicated that this enzyme is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eucalyptus; Protoporphyrinogen IX oxidase; Catalase; Peroxidase; Herbicide. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400009 |
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| Pereira,Tiago Campos; Bittencourt,Vinícius D'Ávila Pascoal; Secolin,Rodrigo; Rocha,Cristiane de Souza; Maia,Ivan de Godoy; Lopes-Cendes,Iscia. |
| The RNA interference (RNAi) technique is a recent technology that uses double-stranded RNA molecules to promote potent and specific gene silencing. The application of this technique to molecular biology has increased considerably, from gene function identification to disease treatment. However, not all small interfering RNAs (siRNAs) are equally efficient, making target selection an essential procedure. Here we present Strand Analysis (SA), a free online software tool able to identify and classify the best RNAi targets based on Gibbs free energy (deltaG). Furthermore, particular features of the software, such as the free energy landscape and deltaG gradient, may be used to shed light on RNA-induced silencing complex (RISC) activity and RNAi mechanisms,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RNAi; SiRNA; SiRNA design; Software. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600030 |
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| Gonçalves,Marcos Cesar; Galdeano,Diogo Manzano; Maia,Ivan de Godoy; Chagas,César Martins. |
| O objetivo deste trabalho foi caracterizar biológica e molecularmente três isolados de Sugarcane mosaic virus (SCMV) de lavouras de milho, analisá-los filogeneticamente e discriminar polimorfismos do genoma. Plantas com sintomas de mosaico e nanismo foram coletadas em lavouras de milho, no Estado de São Paulo e no Município de Rio Verde, GO, e seus extratos foliares foram inoculados em plantas indicadoras e submetidos à análise sorológica com antissoros contra o SCMV, contra o Maize dwarf mosaic virus (MDMV) e contra o Johnsongrass mosaic virus (JGMV). Mudas de sorgo 'Rio' e 'TX 2786' apresentaram sintomas de mosaico após a inoculação dos três isolados, e o DAS-ELISA confirmou a infecção pelo SCMV. O RNA total foi extraído e usado para amplificação por... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Potyviridae; Zea mays; Mosaico comum do milho; RFLP; RT-PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400004 |
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