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Bacterivory by benthic organisms in sediment : Quantification using 15N-enriched bacteria ArchiMer
Pascal, P.y.; Dupuy, C; Mallet, C; Richard, P; Niquil, N.
The fate of benthic bacterial biomass in benthic food webs is a topic of major importance but poorly described. This paper describes an alternative method for evaluation of bacterial grazing rate by meiofauna and macrofauna using bacteria pre-enriched with stable isotopes. Natural bacteria from the sediment of an intertidal mudflat were cultured in a liquid medium enriched with 15NH4Cl. Cultured bacteria contained 2.9% of 15N and were enriched sufficiently to be used as tracers during grazing experiments. Cultured bacteria presented a biovolume (0.21 μm3) and a percentage of actively respiring bacteria (10%) similar to those found in natural communities. The number of Operational Taxon Units found in cultures fluctuated between 56 and 75% of that...
Tipo: Text Palavras-chave: Tracer; Stable isotope; Sediment; Grazing; Bacteria.
Ano: 2008 URL:
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