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pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops Genet. Mol. Biol.
Guidelli,Giovanna V.; Mattiello,Lucia; Gallinari,Rafael H.; Lucca,Paulo Cezar de; Menossi,Marcelo.
Abstract The successful development of genetically engineered monocots using Agrobacterium-mediated transformation has created an increasing demand for compatible vectors. We have developed a new expression vector, pGVG, for efficient transformation and expression of different constructs for gene overexpression and silencing in sugarcane. The pCAMBIA2300 binary vector was modified by adding Gateway recombination sites for fast gene transfer between vectors and the maize polyubiquitin promoter Ubi-1 (ZmUbi1), which is known to drive high gene expression levels in monocots. Transformation efficiency using the pGVG vector reached up to 14 transgenic events per gram of transformed callus. Transgenic plants expressing the β-glucuronidase (GUS) reporter gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monocots; Sugarcane; Vector; Gateway technology; Genetic transformation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300450
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