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Differential capacity of nitrogen assimilation between apical and basal leaf portions of a tank epiphytic bromeliad Braz. J. Plant Physiol.
Takahashi,Cassia A.; Ceccantini,Gregório C.T.; Mercier,Helenice.
The leaf is the main organ of the vegetative body of tank epiphytic bromeliads, which may be subdivided into apical and basal parts. Little is known about the existence of morphological, anatomical or physiological differences between these two leaf portions. The objective of this study was to verify the existence of a differential capacity to assimilate nitrogen from urea between basal and apical leaf portions of Vriesea gigantea, a tank epiphytic bromeliad. Plants were cultivated in vitro in the presence of 5 mM urea for 15 days. During this period, the activities of the enzymes glutamine synthetase (GS, EC 6.3.1.2) and NADH-dependent glutamate dehydrogenase (NADH-GDH, EC 1.4.1.2) were quantified, as well as the densities of trichomes and stomata found...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromeliaceae; Glutamate dehydrogenase; Glutamine synthetase; Stomata; Trichome; Urea.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000200004
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Endogenous auxin and cytokinin contents associated with shoot formation in leaves of pineapple cultured in vitro Braz. J. Plant Physiol.
Mercier,Helenice; Souza,Beatriz Maia; Kraus,Jane Elizabeth; Hamasaki,Regina Mayumi; Sotta,Bruno.
The in vitro culture of pineapple leaves on a shoot induction medium (SIM) results in the formation of protuberances and further development in shoots, and plantlets. The contents of endogenous indoleacetic acid (IAA) and five cytokinins (Cks), N6(2-isopentenyl)adenine (iP), N6(2-isopentenyl)adenosine (iPR), zeatin (Z), zeatin riboside (ZR) and N6-benzyladenine (BA), present in the basal portion of those leaves, were correlated to the organogenic response that occurs over 15 days of culture. The endogenous auxin/cytokinins ratio was lowest on the 3rd day, mainly due to a strong increase in the iP level. It seems that endogenous iP concentration triggered the induction signal for an organogenic response in pineapple leaf bases. The rise in iP content...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ananas comosus; Auxin; Bromeliaceae; Cytokinin; Hormone signalling; Plant regeneration.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000200006
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Glutamine enhances competence for organogenesis in pineapple leaves cultivated in vitro Braz. J. Plant Physiol.
Hamasaki,Regina M.; Purgatto,Eduardo; Mercier,Helenice.
Leaf bases of pineapple cultured on a shoot induction medium (SIM) produced protuberances followed by shoot-buds via direct organogenesis at a frequency of 46 %. When 8 mM glutamine (gln) was a supplement to SIM (SIM8gln), the regeneration rate increased to 70 %, thus suggesting that 8mM gln increased explant competence for organogenesis. Besides this, shoot vigor was strongly enhanced in SIM8gln. Other gln concentrations (16 or 32 mM) evoked a lower frequency of shoot-bud induction and number of regenerated shoots per explant when compared to SIM8gln. In this study, it was defined that explant organogenic commitment to form shoot-buds occurred in the first 7 days of culture on SIM8gln. Thereafter, endogenous indole-3-acetic acid (IAA) and cytokinin (4...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ananas comosus amino acids auxin; Bromeliaceae cytokinins phytohormones shoot regeneration.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000400006
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Sensibilidade estomática ao ABA em função do pH e de níveis de Ca2+, NO3- e PO4(3-) na seiva do xilema Braz. J. Plant Physiol.
Oliveira,Maria Neudes Sousa de; Oliva,Marco Antonio; Martínez,Carlos Alberto; Mercier,Helenice; Silva,Marco Aurélio Pedron e.
A hipótese de que a sensibilidade estomática ao ácido abscísico (ABA) possa ser influenciada pelas características da seiva xilemática, como pH e concentração de Ca2+, NO3- e PO4(3-), foi testada em plantas de Lycopersicon esculentum L. var. Santa Clara submetidas a estresse hídrico induzido pela adição de PEG 6000 à solução nutritiva. Estimou-se a sensibilidade estomática ao ABA pela relação transpiração/ABA xilemático. Plantas intactas foram submetidas a estresse hídrico rápido e lento e determinaram-se as variações de pH e as concentrações de Ca2+, NO3- e PO4(3-) na seiva do xilema Também se estudaram os efeitos de diversas concentrações de Ca2+, NO3- e PO4(3-) sobre a transpiração em folhas destacadas imersas em seiva artificial contendo ABA exógeno....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum L.; Estresse hídrico; Seiva xilemática; Transpiração.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000200006
Registros recuperados: 4
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