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| Fisher, Matthew C.; Ghosh, Pria; Shelton, Jennifer M. G.; Bates, Kieran; Brookes, Lola; Wierzbicki, Claudia; Rosa, Goncalo M.; Farrer, Rhys A.; Aanensen, David M.; Alvarado-rybak, Mario; Bataille, Arnaud; Berger, Lee; Boell, Susanne; Bosch, Jaime; Clare, Frances C.; Courtois, Elodie A.; Crottini, Angelica; Cunningham, Andrew A.; Doherty-bone, Thomas M.; Gebresenbet, Fikirte; Gower, David J.; Hoglund, Jacob; James, Timothy Y; Jenkinson, Thomas S.; Kosch, Tiffany A.; Lambertini, Carolina; Laurila, Anssi; Lin, Chun-fu; Loyau, Adeline; Martel, An; Meurling, Sara; Miaud, Claude; Minting, Pete; Ndriantsoa, Serge; O'Hanlon, Simon J.; Pasmans, Frank; Rakotonanahary, Tsanta; Rabemananjara, Falitiana C. E.; Ribeiro, Luisa P.; Schmeller, Dirk S.; Schmidt, Benedikt R.; Skerratt, Lee; Smith, Freya; Soto-azat, Claudio; Tessa, Giulia; Toledo, Luis Felipe; Valenzuela-sanchez, Andres; Verster, Ruhan; Voeroes, Judit; Waldman, Bruce; Webb, Rebecca J.; Weldon, Che; Wombwell, Emma; Zamudio, Kelly R.; Longcore, Joyce E.; Garner, Trenton W. J.. |
| Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00442/55317/78944.pdf |
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| Faust, Ellika; Andre, Carl; Meurling, Sara; Kochmann, Judith; Christiansen, Henrik; Jensen, Lasse Fast; Charrier, Gregory; Laugen, Ane T.; Strand, Asa. |
| Identifying the routes and rates of introductions is fundamental for the understanding of marine invasions. Recurring introductions over the last 50 yr have led to the establishment of feral Pacific oyster Crassostrea gigas populations throughout Europe. In the northern countries, Sweden and Norway, the species first occurred in large numbers in 2006. Here, we investigated the relative importance of introduction via re-laying of cultured oysters imported for consumption from France, Ireland or the Netherlands, and dispersal of oyster larvae by ocean currents from wild oyster populations in Denmark. Using microsatellite DNA markers, we estimated genetic differentiation among Pacific oysters collected at 4 Swedish locations, 3 Norwegian locations and 9... |
| Tipo: Text |
Palavras-chave: Population genetics; Microsatellites; Range expansion; Non-native species; Aquaculture; Connectivity; Scandinavia; Skagerrak. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00395/50627/72574.pdf |
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