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Genetic Variability of the Tomato Leaf Miner (Tuta absoluta Meirick; Lepidoptera: Gelechiidae), in Tunisia, Inferred from RAPD-PCR 117
Bettaibi,Asma; Mezghani-Khemakhem,Maha; Bouktila,Dhia; Makni,Hanem; Makni,Mohamed.
The tomato leaf miner Tuta absoluta Meyrick has invaded tomato (Solanum lycopersicum L.) crop in Tunisia since 2008 and is representing today a major threat to the production of this crop. In this study, we used the Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technology to assess the genetic variability within and among seven populations of T. absoluta, collected on tomato from different regions in Tunisia. Using five RAPD-PCR primers and 108 individuals, 140 polymorphic fragments were recorded. From 335 different RAPD phenotypes generated, 71 were redundant and 264 unique to a specific population. The genetic structure of T. absoluta was investigated using analysis of molecular variance (AMOVA), genetic distances (Fst) and...
Tipo: Journal article Palavras-chave: Invasive species; Molecular markers; Insect populations; Gene flow.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000200008
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In Silico Identification of MicroRNAs with B/CYDV Gene Silencing Potential 52
Bouallègue,Maryem; Bouktila,Dhia; Mezghani-Khemakhem,Maha; Capy,Pierre; Makni,Mohamed.
ABSTRACT Computational investigation of a set of publicly available plant microRNAs revealed 19 barley- and other plants-encoded miRNAs and their near-complement reverse sequences (miRNA*) that have potential to bind all B/CYDV open reading frames (ORFs) except ORF0 and ORF6. These miRNAs/miRNAs*, their binding positions and targets are discussed in the context of biological protection of cereals against B/CYDV, based on antiviral silencing.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Barley/Cereal Yellow Dwarf virus (B/CYDV); MicroRNA; Gene silencing; Host defense.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100437
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Large-scale analysis of NBS domain-encoding resistance gene analogs in Triticeae 74
Bouktila,Dhia; Khalfallah,Yosra; Habachi-Houimli,Yosra; Mezghani-Khemakhem,Maha; Makni,Mohamed; Makni,Hanem.
Proteins containing nucleotide binding sites (NBS) encoded by plant resistance genes play an important role in the response of plants to a wide array of pathogens. In this paper, an in silico search was conducted in order to identify and characterize members of NBS-encoding gene family in the tribe of Triticeae. A final dataset of 199 sequences was obtained by four search methods. Motif analysis confirmed the general structural organization of the NBS domain in cereals, characterized by the presence of the six commonly conserved motifs: P-loop, RNBS-A, Kinase-2, Kinase-3a, RNBS-C and GLPL. We revealed the existence of 11 distinct distribution patterns of these motifs along the NBS domain. Four additional conserved motifs were shown to be significantly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: NBS domain; Data mining; Phylogeny; Plant resistance genes; Triticeae.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400017
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