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Activity of glycosidases from freshwater heterotrophic microorganisms on the degradation of extracellular polysaccharide produced by Anabaena spiroides (Cyanobacteria) BJM
Colombo,Vanessa; Vieira,Armando A.H.; Moraes,Gilberto.
The activity of specific glycosidases during the degradation of the extracellular polysaccharide (EPS) produced by Anabaena spiroides was determined using MUF-substrates (MUF-monosaccharides). Polysaccharide degradation was found to occur in a two-phase process. The first consisted of high enzymatic activity that consumed 41% of the EPS at a relatively high rate, while the second consumed the remaining polysaccharide (59%) at a slower rate. A transition phase from the higher to the slower degradation rates was marked by a replacement of bacterial populations from coccoid to bacillus cells. During the degradation process, the bacterial biomass increased with the decrease of EPS, as revealed by bacterial cell counts. The enzymatic activity detected through...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anabaena spiroides; Glycosidases; Extracellular polysaccharides; Degradation; Heterotrophic microorganisms.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100018
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Acute toxicity of nitrite to matrinxã, Brycon cephalus (Günther, 1869), (Teleostei-Characidae) Ciência Rural
Avilez,Ive Marchioni; Aguiar,Lucia Helena de; Altran,Alexandre Eneas; Moraes,Gilberto.
Nitrite leads to many physiological and hematological disturbances followed by lethality. This work reports the lethal concentration of nitrite to juvenile matrinxã. Experiments were done at 24ºC, pH 6.7-7.0 under natural photoperiod. Fish were exposed to 0.2- 0.5- 1.0 and 2.0mg L-1 of nitrite N-NO2 for 96h. The 96-h LC50 of nitrite-N was calculated by the trimmed Spearman-Karber method (0.86±0.05mg L-1; 0.57-1.30 to 95%CI) and it was concluded that matrinxã is very sensitive to environmental nitrite. Therefore, care must be taken to prevent accumulation of nitrite in intensive culture systems of matrinxã.
Tipo: Info:eu-repo/semantics/article Palavras-chave: LC50; Nitrite; Matrinxã; Brycon cephalus; Toxicity.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000600013
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Atividade proteolítica e crescimento de matrinxã em natação sustentada e alimentado com dois níveis de proteína PAB
Arbeláez-Rojas,Gustavo Alberto; Inoue,Luís Antônio Kioshi Aoki; Moraes,Gilberto.
O objetivo deste trabalho foi avaliar o efeito da natação sustentada sobre a atividade digestiva proteolítica e o crescimento de juvenis de matrinxã (Brycon amazonicus), alimentados com dois níveis de proteína. Foram utilizados 240 peixes, tratados com 28 ou 38% de proteína bruta (PB), durante 60 dias, em duas situações distintas: em natação sustentada à velocidade de uma vez e meia o comprimento corporal por segundo, e em sistema convencional de cultivo, sem movimento forçado. Os peixes em natação sustentada e alimentados com 28% de PB apresentaram melhor desempenho, expresso como maior crescimento, alta taxa de crescimento específico, maior ganho de peso e melhor eficiência alimentar. A natação sustentada proporcionou aumento significativo da atividade...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brycon amazonicus; Deposição proteica; Enzima digestiva; Exercício físico; Protease; Proteína corporal.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001100014
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Características hematológicas de teleósteos brasileiros: IV. Variáveis do jundiá Rhamdia quelen ( Pimelodidae) Ciência Rural
Tavares-Dias,Marcos; Melo,José Fernando Bibiano; Moraes,Gilberto; Moraes,Flávio Ruas de.
As características hematológicas do jundiá Rhamdia quelen Quoy & Gaimard, 1824 (Osteichthyes: Pimelodidae), oriundo de cativeiro foram estudadas. Determinou-se o valor médio da contagem de eritrócitos, taxa de hemoglobina, hematócrito, volume corpuscular médio (VCM), concentração de hemoglobina corpuscular média (CHCM) e distribuição percentual de células sangüíneas de defesa orgânica. As células sangüíneas de defesa orgânica (leucócitos e trombócitos) foram identificadas e caracterizadas morfologicamente. Trombócitos e linfócitos foram as células sangüíneas de defesa orgânica mais freqüente nas extensões sangüíneas do R. quelen. A análise de regressão mostrou correlação linear negativa entre o percentual de trombócitos e linfócitos. Os resultados...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hematologia; Leucócitos; Peixe de água doce; Rhamdia quelen; Sangue.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000400024
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Clove oil as anaesthetic for juveniles of matrinxã Brycon cephalus (Gunther, 1869) Ciência Rural
Inoue,Luis Antônio Kioshi Aoki; Santos Neto,Cristiano dos; Moraes,Gilberto.
Many chemicals have been used as anaesthetics in fish farms and fish biology laboratories to keep the fish immobilized during handling procedures and to prevent accidents and animal stress. In Brazil, tricaine methane sulfonate (MS 222), quinaldine sulfate, benzocaine, and phenoxyethanol are the most common fish anaesthetics used to prevent fish stress during handling, but many side effects such as body and gill irritations, corneal damage and general risks of intoxication have been reported. Clove oil is a natural product proposed as an alternative fish anaesthetic by many researchers and it has been used in many countries with great economic advantages and no apparent toxic properties. In this work, we assessed the suitability of clove oil to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clove oil; Anaesthetic; Matrinxã.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000500023
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Effect of methyl parathion on the muscle and brain acetylcholinesterase activity of matrinxã (Brycon cephalus) Ciência Rural
Almeida,Luciana Cristina de; Aguiar,Lúcia Helena; Moraes,Gilberto.
Farming of the freshwater fish is emerging in Brazil and many species from the wild are promising. The teleost matrinxã (Brycon cephalus) holds several characteristics such as fast growth rate, high commercial value and adaptability to artificial raring conditions, which make it a promising species for commerce. The use of pesticides in aquatic environment is frequent in Brazil, and methyl parathion is very common in aquaculture. We have determined the enzymatic activity of acetyl cholinesterase in white muscle and brain of matrinxã exposed to 2ppm of environmental methyl parathion for 24 hours. There was 64% and 69% of acetyl cholinesterase inhibition in muscle and brain respectively. These activities were not recovered after 8 days from exposure to this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetylcholinesterase; Methyl parathion; Matrinxã; Toxicity.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000600029
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Effects of clove oil on the stress response of matrinxã (Brycon cephalus) subjected to transport Acta Amazonica
Inoue,Luís Antônio Kioshi Aoki; Afonso,Luís Orlando B.; Iwama,George K.; Moraes,Gilberto.
Fish transport is one of the most stressful procedures in aquaculture facilities. The present work evaluated the stress response of matrinxã to transportation procedures, and the use of clove oil as an alternative to reduce the stress response to transport in matrinxã (Brycon cephalus). Clove oil solutions were tested in concentrations of 0, 1, 5 and 10 mg/L during matrinxã transportation in plastic bags, supplied with water and oxygen as the usual field procedures in Brazil. Clove oil reduced some of the physiological stress responses (plasma cortisol, glucose and ions) that we measured. The high energetic cost to matrinxã cope with the transport stress was clear by the decrease of liver glycogen after transport. Our results suggest that clove oil (5...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Matrinxã Brycon cephalus; Transport; Stress; Clove oil.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672005000200018
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Gastrointestinal transit of extruded or pelletized diets in pacu fed distinct inclusion levels of lipid and carbohydrate PAB
Honorato,Claucia Aparecida; Almeida,Luciana Cristina de; Nunes,Cleujosí da Silva; Carrilho,Elma Neide Vasconcelos Martins; Moraes,Gilberto.
The objective of this work was to evaluate the effect of pelletized or extruded diets, with different levels of carbohydrate and lipid, on the gastrointestinal transit time (GITT) and its modulation in pacu (Piaractus mesopotamicus). One hundred and eighty pacu juveniles were fed with eight isonitrogenous diets containing two carbohydrate levels (40 and 50%) and two lipid levels (4 and 8%). Four diets were pelletized and four were extruded. Carbohydrate and lipid experimental levels caused no changes to the bolus transit time. However, the bolus permanence time was related to diet processing. Fish fed pelletized diets exhibited the highest gastrointestinal transit time. Regression analysis of bolus behavior for pelletized and extruded diets with 4% lipid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Piaractus mesopotamicus; Carbohydrate; Lipid; Starch gelatinization; Viscosity..
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2014001100829
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Growth and hematology of pacu subjected to sustained swimming and fed different protein levels PAB
Nunes,Cleujosí da Silva; Moraes,Gilberto; Fabrizzi,Fernando; Hackbarth,Araceli; Arbeláez-Rojas,Gustavo Alberto.
The objective of this work was to evaluate the effect of sustained swimming and dietary protein levels on growth and hematological responses of juvenile pacu (Piaractus mesopotamicus). A completely randomized design was used in a 3x2 factorial arrangement, with three levels of dietary protein (24, 28, and 32% crude protein), two rearing conditions (sustained swimming or motionless water), and 15 replicates. Fish were subjected to sustained swimming at the velocity of two body lengths per second (2 BL s-1), for 45 days. The level of dietary protein and the swimming conditions affected the performance, growth, and hematological profile of pacu. Swimming conditions influenced nutritional factors, increasing daily weight gain, specific growth rate, number of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Piaractus mesopotamicus; Erythropoiesis; Exercise; Growth rate; Weight gain.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000600010
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Interação do exercício de natação sustentada e da densidade de estocagem no desempenho e na composição corporal de juvenis de matrinxã Brycon amazonicus Ciência Rural
Arbeláez-Rojas,Gustavo Alberto; Moraes,Gilberto.
O objetivo deste estudo foi estimar o efeito da densidade de estocagem associada ao exercício de natação moderada no desempenho e na composição corporal de juvenis de matrinxã (Brycon amazonicus). Foram utilizados peixes com comprimento e peso médio inicial de 12,3±0,5cm e 18,4±0,1g, os quais foram distribuídos ao acaso em dois grupos: o primeiro grupo de peixes foi estocado em três densidades correspondendo a 88, 176 e 353 peixes m-3 e foi condicionado a nadar a uma velocidade de 1,0cc s-1 (comprimento corporal por segundo) em tanques circulares de 250L, durante 70 dias. O segundo grupo de peixes foi mantido nas mesmas densidades em água parada (sem exercício) perfazendo no total seis tratamentos com três repetições. Foram estimados parâmetros de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brycon amazonicus; Recria de peixes; Cultivo intensivo; Raceway; Gordura; Matrinxã.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100031
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Intermittent sustained swimming in 'matrinxã' Brycon amazonicus (Bryconidae: Bryconinae): hematological and metabolic responses Neotropical Ichthyology
Fabrizzi,Fernando; Moraes,Gilberto; Hackbarth,Araceli; Almeida,Luciana Cristina de; Arbeláez-Rojas,Gustavo; Nunes,Cleujosí da Silva.
In fish, studies on a wide variety of physiological effects of exercise have been reported since a long time. It has been attributed special attention to some types of exercise, however, its application as a healthful practice in the rearing and welfare of farming fish is rising in last few years. In this particular, long-term intermittent sustained swimming (ISS) has been not yet explored. In this work, the freshwater fish Brycon amazonicus was submitted to (ISS) for 30 days at velocity of 1.0 body-length sec-1 for 12h interspaced by 12h under still water. Hematology and metabolism were evaluated. Exercised fish decreased 30% the erythrocyte number and hemoglobin was unvaried. The stores of liver glycogen and muscular triacylglycerol (TAG) were increased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adaptation; Exercise; Fish; Glycogen; Metabolism; Triacylglycerol.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000200425
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Metabolic responses of channel catfish (Ictalurus punctatus) exposed to phenol and post-exposure recovery Anais da ABC (AABC)
Moraes,Fernanda D.; Rossi,Priscila A.; Figueiredo,Juliana S.L.; Venturini,Francine P.; Cortella,Lucas R.X.; Moraes,Gilberto.
Metabolic adjustments were studied in channel catfish Ictalurus punctatus exposed to 1.5 mg L-1 of phe nol (10% LC50) for four days and recovered for seven days. Lower triacylglycerol (TGA) stores and increased muscle fat free acids (FFA) suggest fat catabolism in muscle. Remarkable liver FFA decrease (-31%) suggests liver fat catabolism as well. Increased muscular ammonia levels and ASAT (aspartate aminotransferase) and decreased plasma aminoacids suggest higher muscular amino acid uptake. Constant levels of glucose and increased liver glycogen stores, associated with lower amino acids in plasma, indicate gluconeogenesis from amino acids. This is supported by higher hepatic ALAT and ASAT. Higher hepatic LDH followed by lower plasma lactate may indicate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metabolic adjustments; Enzymes; Lipid stores; Intermediary metabolism; Recovery; Xenobiotic.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000300865
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Optimization of sustaining swimming speed of matrinxã Brycon amazonicus: performance and adaptive aspects Scientia Agricola
Arbeláez-Rojas,Gustavo; Moraes,Gilberto.
Deleterious changes in metabolism, growth performance and body composition may be observed if fish are constrained to swimming continuously or intermittently at over-speeds. This study evaluates effects of four water speeds on growth, body composition and hematologic profile of juvenile matrinxã, Brycon amazonicus. Fish (33.3 ± 0.9 g and 13.44 ± 0.1 cm) were held for 90 days in five water speeds (0.0 - control, 1.0, 1.5, 2.0 and 2.5 body lengths per second - BLAt swimming speeds ranging on 1.0 and 1.5 BL s–1, in fish growth was 20% higher. Hemoglobin and red blood cells at 1.5 BL s–1 increased 24% and 18% respectively; hematocrit was 17% higher in all exercised fish; protein content of white muscle at 1.0 BL s–1 was 2% higher; lipid deposition in red...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Body composition; Exercise; Fish growth; Hematology; Sustained swimming.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000300001
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Physiological stress responses in the warm-water fish matrinxã (Brycon amazonicus ) subjected to a sudden cold shock Acta Amazonica
Inoue,Luis Antonio Kioshi Aoki; Moraes,Gilberto; Iwama,George K.; Afonso,Luis Orlando Bertola.
The present work evaluated several aspects of the generalized stress response [endocrine (cortisol), metabolic (glucose), hematologic (hematocrit and hemoglobin) and cellular (HSP70)] in the Amazonian warm-water fish matrinxã (Brycon amazonicus ) subjected to an acute cold shock. This species farming has been done in South America, and growth and feed conversion rates have been interesting. However, in subtropical areas of Brazil, where the water temperature can rapidly change, high rates of matrinxã mortality have been associated with abrupt decrease in the water temperature. Thus, we subjected matrinxã to a sudden cold shock by transferring the fish directly to tanks in which the water temperature was 10ºC below the initial conditions (cold shock from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Matrinxã; Cold shock; Stress; HSP70.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000400002
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Standardization of 2-phenoxyethanol as anesthetic for juvenile Brycon cephalus (Gunther, 1869): the use in field procedures Ciência Rural
Inoue,Luis Antônio Kioshi Aoki; Santos Neto,Cristiano dos; Moraes,Gilberto.
Hyper motility is a negative factor in fish handling procedures due to the risks of damages to the animals. Chemicals are widely used to anesthetize fish during biometry and many other handling procedures. 2-Phenoxyethanol is largely employed, but many studies are necessary about tropical fish responses. The present research evaluated the anesthesia induction time for juvenile matrinxã submitted to eight different levels of 2-phenoxyethanol. The range of 2-phenoxyethanol concentration was 250-600mg liter-1. Induction time of anesthesia decreased as a function of 2-phenoxyethanol concentrations. Fish were safely anesthetized approximately after one minute of exposure to 2-phenoxyethanol batches in concentrations above 400mg/liter, and the recover period was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-phenoxyethanol; Anesthetic; Brycon cephalus; Matrinxã.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000200035
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Sustained swimming improves fish dietary nutrient assimilation efficiency and body composition of juvenile Brycon amazonicus Scientia Agricola
Arbeláez-Rojas,Gustavo Alberto; Moraes,Gilberto.
ABSTRACT Sustained swimming (SS) usually promotes beneficial effects in growth and feed conversion of fishes. Although feed efficiency is improves at moderate water velocity, more information is required to determine the contributions of this factor on growth and body composition. Body composition and efficiency responses to the use of nutrients were determined in juvenile matrinxa Brycon amazonicus (Spix and Agassiz, 1829) fed with two dietary amounts of protein, 28 or 38% of crude protein (CP), and subjected to sustained swimming at a constant speed of 1.5 body lengths s−1 (BL s−1) or let to free swimming. The fish body composition under SS and fed with 28% of dietary protein showed 22% of increased in bulk protein and a 26% of decrease in water content...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Benefits of exercise; Dietary protein; Nutrient retention; Optimal swimming speeds; Raceways.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000400259
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Sustained swimming mitigates stress in juvenile Brycon amazonicus reared in high stocking densities PAB
Arbeláez-Rojas,Gustavo Alberto; Moraes,Gilberto; Nunes,Cleujosí da Silva; Fabrizzi,Fernando.
Abstract: The objective of this work was to evaluate the effect of stocking density associated with the swimming exercise on the stress responses of Brycon amazonicus. During 70 days, fish were subjected to three stocking densities: LD, low density of 88 fish per cubic meter; ID, intermediary density of 176 fish per cubic meter; and HD, high density of 353 fish per cubic meter. These densities were combined with static water (non-exercised group) or moderate-speed water (exercised group). Chronic stress was observed in HD, and plasma cortisol and glucose increased with the stocking densities. In HD, levels of plasma cortisol were significantly lower in exercised fish (135 ng mL-1) than in non-exercised ones (153 ng mL-1). The greatest hepatic glycogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal welfare; Intensive fish farming; Metabolic responses; Physical exercise..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000100001
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