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	Provedor de dados BJM (5) Autor Nakano,Viviane (5) Avila-Campos,Mario Julio (3) Avila-Campos,Mario J. (2) Ignacio,Aline (2) Cabral,Fátima C. (1) Fernandes,Miriam R. (1) Fernandes,Miriam Rodriguez (1) Fukugaiti,Márcia H. (1) Gaetti-Jardim Jr.,Elerson (1) Gaetti-Jardim Júnior,Elerson (1) Gamba,Rosa (1) Llanco,Luis A. (1) Marques,Márcia Martins (1) Moraes,Claudia T.P. de (1) Piazza,Roxane M.F. (1) Ribeiro Júnior,Ulysses (1) Tomazinho,Paulo Henrique (1) Wahasugui,Thais C. (1) Wahasugui,Thais Cristiane (1) Mais... Palavra-chave Adhesion (1) Bacteroides fragilis (1) Biotype (1) Candida albicans (1) Children (1) Clostridium perfringens type A (1) Colorectal cancer (1) ETBF (1) Enterobacteria (1) Enterococci (1) HIV patients (1) Intestinal microbiota (1) Invasion (1) Leukotoxin (1) NTBF (1) Necrotic enteritis (1) Oral and intestinal bacteria (1) Periodontitis (1) TpeL gene (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Ano 2017 (1) 2015 (2) 2008 (2) País Brazil (5) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Adhesion and invasion of Clostridium perfringens type A into epithelial cells BJM Llanco,Luis A.; Nakano,Viviane; Moraes,Claudia T.P. de; Piazza,Roxane M.F.; Avila-Campos,Mario J.. ABSTRACT Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and α- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated. Six (27.3%) of the 22 C. perfringens type A strains were harboring the tpeL gene and produced morphological alterations in Vero cells after 6 h of incubation. Strains tpeL (-) induced strong cell rounding after 6 h of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Invasion; Clostridium perfringens type A; Necrotic enteritis; TpeL gene. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400764 Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis BJM Gaetti-Jardim Jr.,Elerson; Wahasugui,Thais Cristiane; Tomazinho,Paulo Henrique; Marques,Márcia Martins; Nakano,Viviane; Avila-Campos,Mario Julio. Aggregatibacter actinomycetemcomitans is an important etiologic agent of the periodontitis and is associated with extra-oral infections. In this study, the detection of the ltxA gene as well as the ltx promoter region from leukotoxic A. actinomycetemcomitans isolated from 50 Brazilian patients with periodontitis and 50 healthy subjects was performed. The leukotoxic activity on HL-60 cells was also evaluated. Leukotoxic activity was determined using a trypan blue exclusion method. The 530 bp deletion in the promoter region was evaluated by PCR using a PRO primer pair. A. actinomycetemcomitans was detected by culture and directly from crude subgingival biofilm by PCR using specific primers. By culture, A. actinomycetemcomitans was detected in nine (18%) of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotype; Leukotoxin; Periodontitis. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400011 Enterotoxigenic and non-enterotoxigenic Bacteroides fragilis from fecal microbiota of children BJM Ignacio,Aline; Fernandes,Miriam Rodriguez; Avila-Campos,Mario Julio; Nakano,Viviane. Enterotoxigenic Bacteroides fragilis (ETBF) is an important part of the human and animal intestinal microbiota and is commonly associated with diarrhea. ETBF strains produce an enterotoxin encoded by the bft gene located in the B. fragilis pathogenicity island (BfPAI). Non-enterotoxigenic B. fragilis (NTBF) strains lack the BfPAI and usually show two different genetic patterns, II and III, based on the absence or presence of a BfPAI-flanking region, respectively. The incidence of ETBF and NTBF strains in fecal samples isolated from children without acute diarrhea or any other intestinal disorders was determined. All 84 fecal samples evaluated were B. fragilis-positive by PCR, four of them harbored the bft gene, 27 contained the NTBF pattern III DNA... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteroides fragilis; Children; ETBF; NTBF. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401141 High occurrence of Fusobacterium nucleatum and Clostridium difficile in the intestinal microbiota of colorectal carcinoma patients BJM Fukugaiti,Márcia H.; Ignacio,Aline; Fernandes,Miriam R.; Ribeiro Júnior,Ulysses; Nakano,Viviane; Avila-Campos,Mario J.. Abstract Colorectal carcinoma is considered the fourth leading cause of cancer deaths worldwide. Several microorganisms have been associated with carcinogenesis, including Enterococcus spp., Helicobacter pylori, enterotoxigenic Bacteroides fragilis, pathogenic E. coli strains and oral Fusobacterium. Here we qualitatively and quantitatively evaluated the presence of oral and intestinal microorganisms in the fecal microbiota of colorectal cancer patients and healthy controls. Seventeen patients (between 49 and 70 years-old) visiting the Cancer Institute of the Sao Paulo State were selected, 7 of whom were diagnosed with colorectal carcinoma. Bacterial detection was performed by qRT-PCR. Although all of the tested bacteria were detected in the majority of the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Oral and intestinal bacteria; Colorectal cancer; Intestinal microbiota. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401135 Occurrence of yeasts, enterococci and other enteric bacteria in subgingival biofilm of HIV-positive patients with chronic gingivitis and necrotizing periodontitis BJM Gaetti-Jardim Júnior,Elerson; Nakano,Viviane; Wahasugui,Thais C.; Cabral,Fátima C.; Gamba,Rosa; Avila-Campos,Mario Julio. The purpose of this study was to determine the prevalence of enteric bacteria and yeasts in biofilm of 80 HIV-positive patients with plaque-associated gingivitis or necrotizing periodontitis. Patients were subjected to extra, intra oral and radiographic examinations. The oral hygiene, bleeding on probing, gingival conditions, and attachment loss were evaluated. Clinical specimens were collected from gingival crevices or periodontal pockets, transferred to VMGA III, diluted and transferred to Sabouraud Dextrose agar with 100 µg/ml of chloramphenicol, peptone water, EVA broth, EMB agar, SS agar, Bile esculin agar and Brilliant green agar. Isolation of yeasts was carried out at room temperature, for 3-7 days; and for the isolation of enteric microorganisms... Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV patients; Candida albicans; Enterococci; Enterobacteria. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200011 Registros recuperados: 5 Primeira ... 1 ... Última

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