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Amplification of the cap20 pathogenicity gene and genetic characterization using different markers molecular in Colletotrichum gloeosporioides isolates BABT
Maciel,Danielli Barreto; Medeiros,Lílian Vieira de; Medeiros,Vivian Vieira de; Leão,Mariele Porto Carneiro; Camargo,Luis Eduardo Aranha; Oliveira,Neiva Tinti de.
Studies were performed to analyze the genetic characterization using RFLP-ITS and Intron (primer EI1) markers and the amplification of the cap20 pathogenicity gene by PCR in Colletotrichum gloeosporioides isolates of different hosts plant. The genetic variability was accessed using RFLP-ITS and Intron markers and grouping by UPGMA method. Primers to cap20 gene were constructed using selected sequences of the GenBank (National Center of Biotechnology Information, http://www.ncbi.nlm.nih.gov) with the Primer 3 program. The dendrograms analysis showed that the RFLP-ITS marker was more informative to separate the Colletotrichum sp, and that primer EI1 demonstrated greater genetic diversity. The amplification of the DNA of the Colletotrichum isolates to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cap20 gene; Colletotrichum gloeosporioides; Pathogenicity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600001
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Biological and chemical control of Sclerotinia sclerotiorum using Trichoderma spp. and Ulocladium atrum and pathogenicity to bean plants BABT
Figueirêdo,Girlene Soares de; Figueirêdo,Lívio Carvalho de; Cavalcanti,Francinete Carla Nunes; Santos,Angela Coimbra dos; Costa,Antonio Felix da; Oliveira,Neiva Tinti de.
Four isolates of Sclerotinia sclerotiorum were tested for pathogenicity in IPA-10 variety bean plants (Phaseolus vulgaris L.), and all were pathogenic. Biological control in vitro was evaluated using eight isolates of Trichoderma spp. and, one of Ulocladium atrum. Chemical control in vitro with fungicides Thiophanate methyl, Iprodione and Carbendazim was also tested. Except U. atrum, all Trichoderma isolates showed antagonistic potential against S. sclerotiorum, where isolate 3601 presented the best performance. Thiophanate methyl chemical control was the most efficient. This fungicide and isolate 3601were compared in vivo in greenhouse. There was statistical difference between the treatments, and the application of fungicide and antagonist before the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: S. sclerotiorum; Trichoderma; Antagonist; Bean plant.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100001
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Biological insect control using Metarhizium anisopliae: morphological, molecular, and ecological aspects Ciência Rural
Tiago,Patricia Vieira; Oliveira,Neiva Tinti de; Lima,Elza Áurea de Luna Alves.
Microbial control of insects is based on the rational use of pathogens to maintain environmentally balanced pest population levels, and Metarhizium anisopliae has been the most studied and most utilized fungal species for that purpose. The natural genetic variability of entomopathogenic fungi is considered one of the principal advantages of microbial insect control. The inter- and intraspecific variability and the genetic diversity and population structures of Metarhizium and other entomopathogenic fungi have been examined using ITS-RFLP, ISSR, and ISSP molecular markers. The persistence of M. anisopliae in the soil and its possible effects on the structures of resident microbial communities must be considered when selecting isolates for biological insect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Metarhizium anisopliae; Molecular markers.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400012
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Cellulase activity of a Lentinula edodes (Berk.) Pegl. strain grown in media containing Carboximetilcellulose or microcrystalline cellulose BABT
Pereira Júnior,José Antônio de Sousa; Correia,Marcos José; Oliveira,Neiva Tinti de.
Endoglucanase and exocellobiohydrolase produced b Lentinula edodes (Berk.) Pegl. strain thatt was cultivated in carboxymetilcellulose (CMC) or microcrystalline cellulose (Avicel) liquid media. The concentration and type of cellulose influenced the enzyme activity and production. Extra-cellular cellobiase activity was not detected in CMC or Avicel media. This enzyme was detected in mycelial extracts only. With 1.7% Avicel liquid medium, the strain did not produce exocellobiohydrolase, but 74 µmol RBBR/mg protein/min was detected with 0.5% Avicel. The substitution of Avicel by 0.5% CMC reduced this activity. Endoglucanase also had maximum activity in 0.5% Avicel medium (approximately 820 UI/mg protein) after 96 h incubation. In supernatants from 0.5% CMC,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lentinula edodes; Cellulase; Liquid medium cultivation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300004
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Comparação da eficiência de métodos de inoculação na avaliação da patogenicidade de isolados de colletotrichum gloeosporioides em frutos de maracujá (Passiflora Edulis) BABT
Rocha,José de Ribamar de Sousa; Oliveira,Neiva Tinti de; Menezes,Maria.
Eight inoculation methods were studied to evaluate the pathogenicity among six isolates of Colletotrichum gloeosporioides, agent of anthracnose on passion fruits (Passiflora edulis). The isolates were selected by micelial growth and sporulation. The inoculations were made through suspension (1x10(6) conidia/ml) and micelial-agar discs (4mm in diameter). After 6 days of inoculation, the diameter of necrotic area was measure on the fruit epiderm. The most efficient inoculation method was the micelial-agar disc on the fruit wounded epiderm. All isolates caused necrosis by this method, but it showed low efficiency using intact epiderm, where only one isolate was active. One isolate from stem showed greater pathogenicity than the others, and the necrosis in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colletotrichum gloeosporioides; Maracujá; Patogenicidade.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100014
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Differential pathogenicity of Metarhizium anisopliae and the control of the sugarcane root spittlebug Mahanarva fimbriolata BABT
Tiago,Patricia Vieira; Souza,Hilton Marcelo de Lima; Moysés,Juliano Borsato; Oliveira,Neiva Tinti de; Lima,Elza Áurea de Luna Alves.
In order to assess the effectiveness of Metarhizium anisopliae var. anisopliae (Metschnikoff) Sorokin isolates in controlling the sugarcane root spittlebug Mahanarva fimbriolata (Stal) (Hemiptera: Cercopidae), nine isolates obtained from a single geographical region were studied. 'Confirmed cumulative' and 'corrected cumulative' spittlebug mortality rates were measured for each of the isolates. Based on the confirmed mortality curve, the isolates URM5946, URM5951 and URM6033 were considered to be potentially the most effective in a biological control program for M. fimbriolata.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Entomopathogenic fungus; Metarhizium anisopliae; Mahanarva fimbriolata.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300002
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DNA polymorphism and total protein in mutants of Metarhizium anisopliae var. Anisopliae (Metsch.) Sorokin strain E9 BJM
Freire,Laurineide Lopes de Carvalho; Costa,Ana Bolena Lima da; Góes,Larissa Brandão; Oliveira,Neiva Tinti de.
Five mutants (MaE10, MaE27, MaE24, MaE41 e MaE49) of Metarhizium anisopliae wild strain E9 were analysed for DNA profile through the RAPD technique and for changes in total protein content by spectrophotometry, polyacrylamide gel electrophoresis and densitometry. The pattern of RAPD markers showed genetic polymorphism among the strains: out of twenty primers seven were selected, producing 113 bands. Forty seven bands were present in all strains (41.6% of monomorphic bands) and 66 showed polymorphism (58.4%). The mean coefficient of similarity among all strains was 0.75 (75%). The total protein content varied, staining in the interval of 6.0-8.0 µg/µl. The electrophoresis analysis, through zymogram and protein fraction profiles by densitometry, allowed the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metarhizium anisopliae; Mutants; RAPD; Total protein.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200004
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Elastin increases biofilm and extracellular matrix production of Aspergillus fumigatus BJM
Brandão,Ildnay de Souza Lima; Oliveira-Moraes,Heloiza Maria da Silva; Motta,Cristina Maria de Souza; Oliveira,Neiva Tinti de; Magalhães,Oliane Maria Correia.
Abstract Aspergillus fumigatus is an opportunistic saprobe fungus that accounts for 90% of cases of pulmonary aspergillosis in immunosuppressed patients and is known for its angiotropism. When it reaches the respiratory tract, A. fumigatus interacts with structural components and blood vessels of the lungs, such as elastin. To understand the effect of this structural component, we examined the effect of elastin on the production and development of the biofilm of A. fumigatus. In RPMI containing 10 mg/mL of elastin, a significant increase (absorbance p < 0.0001; dry weight p < 0.0001) in the production of biofilm was observed in comparison to when RPMI was used alone, reaching a maximum growth of 18.8 mg (dry weight) of biofilm in 72 h. In addition,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus fumigatus; Biofilm; Elastin; Extracellular matrix; Hydrophobicity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300675
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Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers BJM
Brasileiro,Bereneuza Tavares Ramos Valente; Coimbra,Maria Raquel Moura; Morais Jr,Marcos Antonio de; Oliveira,Neiva Tinti de.
Fusarium solani fungus (teleomorph Haematonectria haematococca) is of relevance for agriculture, producing a disease that causes significant losses for many cultivars. Moreover, F. solani is an opportunistic pathogen to animals and humans. The complexity associated to its correct identification by traditional methods justifies the efforts of using molecular markers for isolates characterization. In this work, three PCR-based methods (one PCR-ribotyping and two PCR-fingerprinting) were used to investigate the molecular variability of eighteen F. solani isolates from four Brazilian States, collected from different substrates. Genetic analysis revealed the intraspecific variability within the F. solani isolates, without any correlation to their geographical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Intron splice site; Fusarium solani; Microsatellite; PCR-fingerprinting; Ribosomal DNA.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200006
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Randomly Amplified Polymorphic DNA of Trichoderma isolates and antagonism against Rhizoctonia solani BABT
Góes,Larissa Brandão; Costa,Ana Bolena Lima da; Freire,Laurineide Lopes de Carvalho; Oliveira,Neiva Tinti de.
Random Amplified Polymorphic DNA (RAPD) procedure was used to examine the genetic variability among fourteen isolates of Trichoderma and their ability to antagonize Rhizoctonia solani using a dual-culture assay for correlation among RAPD products and their hardness to R. solani. Seven oligodeoxynucleotide primers were selected for the RAPD assays which resulted in 197 bands for 14 isolates of Trichoderma. The data were entered into a binary matrix and a similarity matrix was constructed using DICE similarity (SD) index. A UPGMA cluster based on SD values was generated using NTSYS (Numerical Taxonomy System, Applied Biostatistics) computer program. A mean coefficient of similarity obtained for pairwise comparisons among the most antagonics isolates was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RAPD; Trichoderma; Rhizoctonia solani.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200005
Registros recuperados: 10
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