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Registros recuperados: 11
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Agronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene PAB
Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Salvadori,José Roberto; Nicolau,Marcio; Kaltchuk-Santos,Eliane; Alves,Laura Bianchessi; Bodanese-Zanettini,Maria Helena.
The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticarsia gemmatalis; Glycine max; Plant resistance to insects; Gene transfer.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700003
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Amplification of 16S rRNA gene sequences to differentiate two highly related bradyrhizobia species PAB
Giongo,Adriana; Ambrosini,Adriana; Freire,João Ruy Jardim; Zanettini,Maria Helena Bodanese; Passaglia,Luciane Maria Pereira.
A 16S rRNA gene PCR-based assay was developed aiming at a fast molecular diagnostic method to differentiate the two phylogenetically closely related species Bradyrhizobium japonicum and B. elkanii, isolated from soybean nodules, in order to identify those more competitive and comprising greater nitrogen fixation ability for use in the formulation of commercial inoculants. The assay used was able to discriminate ten reference strains belonging to these two Bradyrhizobium species, as well as to efficiently identify 37 strains isolated from fields cultivated with soybean.
Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR; Rhizobial diversity; Commercial inoculants.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000900019
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Application of representational difference analysis to identify genomic differences between Bradyrhizobium elkanii and B. Japonicum species BJM
Soares,René Arderius; Passaglia,Luciane Maria Pereira.
Bradyrhizobium elkanii is successfully used in the formulation of commercial inoculants and, together with B. japonicum, it fully supplies the plant nitrogen demands. Despite the similarity between B. japonicum and B. elkanii species, several works demonstrated genetic and physiological differences between them. In this work Representational Difference Analysis (RDA) was used for genomic comparison between B. elkanii SEMIA 587, a crop inoculant strain, and B. japonicum USDA 110, a reference strain. Two hundred sequences were obtained. From these, 46 sequences belonged exclusively to the genome of B. elkanii strain, and 154 showed similarity to sequences from B. japonicum genome. From the 46 sequences with no similarity to sequences from B. japonicum, 39...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bradyrhizobium; Genome comparison; Nitrogen fixation; Representational difference analysis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400036
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Characterization of plant growth-promoting bacteria inhabiting Vriesea gigantea Gaud. and Tillandsia aeranthos (Loiseleur) L.B. Smith (Bromeliaceae) Biota Neotropica
Giongo,Adriana; Beneduzi,Anelise; Gano,Kelsey; Vargas,Luciano Kayser; Utz,Laura; Passaglia,Luciane Maria Pereira.
Microorganisms that live inside and around a plant can supply it with essential substances, such as phytohormones and essential nutrients. The present investigation aimed to isolate and characterize the phyllosphere, the endophytic, and the water tank bacteria associated with Vriesea gigantea and Tillandsia aeranthos. The bacteria were tested for siderophore and indole-3-acetic acid (IAA) production, phosphate solubilization, and presence of the nif H gene. Genetic diversity of the bacterial isolates was evaluated by rep-PCR. Sixty-eight bacterial strains were isolated from 3 different microhabitats of V. gigantea and from 2 microhabitats of T. aeranthos bromeliad plants. Gram-positive, spore-forming bacilli comprised most bacterial isolates. All isolates...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromeliads; PGPB; Diazotrophic bacteria; Water tank; Siderophore; IAA.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032013000300080
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Diversidade de bactérias diazotróficas endofíticas associadas a plantas de milho Rev. Bras. Ciênc. Solo
Roesch,Luiz Fernando Wurdig; Passaglia,Luciane Maria Pereira; Bento,Fátima Menezes; Triplett,Eric W.; Camargo,Flávio Anastácio Oliveira.
Bactérias diazotróficas endofíticas são capazes de promover o crescimento do milho por meio da fixação biológica do nitrogênio (FBN) ou pela produção de fitormônios. Neste estudo, objetivou-se caracterizar a diversidade de bactérias diazotróficas endofíticas associadas a plantas de milho em diferentes locais do Rio Grande do Sul, que apresentavam variações de clima e solo. Para isso, foi usado um método baseado na amplificação do gene nifH grupo I, na análise de fragmentos de restrição (PCR-RFLP) e no seqüenciamento dos genes amplificados. Foram calculados os índices de Shannon-Weaver e Equitabilidade para estimar a diversidade dos diazotróficos, bem como a diversidade de nucleotídeos e divergência entre seqüências, para estimar a diversidade genética das...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene nifH; PCR-RFLP; Seqüenciamento; Comunidade endofítica.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832007000600015
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Identification of the soybean HyPRP family and specific gene response to Asian soybean rust disease Genet. Mol. Biol.
Bücker Neto,Lauro; Oliveira,Rafael Rodrigues de; Wiebke-Strohm,Beatriz; Bencke,Marta; Weber,Ricardo Luís Mayer; Cabreira,Caroline; Abdelnoor,Ricardo Vilela; Marcelino,Francismar Correa; Zanettini,Maria Helena Bodanese; Passaglia,Luciane Maria Pereira.
Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungal disease; HyPRP genes; Glycine max; Real time RT-qPCR.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200012
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Isolation and characterization of two plant growth-promoting bacteria from the rhizoplane of a legume (Lupinus albescens) in sandy soil Rev. Bras. Ciênc. Solo
Giongo,Adriana; Beneduzi,Anelise; Ambrosini,Adriana; Vargas,Luciano Kayser; Stroschein,Marcos Roberto; Eltz,Flávio Luiz; Bodanese-Zanettini,Maria Helena; Passaglia,Luciane Maria Pereira.
Two bacterial strains that amplified part of the nifH gene, RP1p and RP2p, belonging to the genus Enterobacter and Serratia, were isolated from the rhizoplane of Lupinus albescens. These bacteria are Gram-negative, rod-shaped, motile, facultative anaerobic, and fast-growing; the colonies reach diameters of 3-4 mm within 24 h of incubation at 28 ºC. The bacteria were also able to grow at temperatures as high as 40 ºC, in the presence of high (2-3 % w/v) NaCl concentrations and pH 4 -10. Strain RP1p was able to utilize 10 of 14 C sources, while RP2p utilized nine. The isolates produced siderophores and indolic compounds, but none of them was able to solubilize phosphate. Inoculation of L. albescens with RP1p and RP2p strains resulted in a significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Indolic compounds; Siderophores; Inoculation experiment.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000200009
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Occurrence of plant growth-promoting traits in clover-nodulating rhizobia strains isolated from different soils in Rio Grande do Sul state Rev. Bras. Ciênc. Solo
Vargas,Luciano Kayser; Lisboa,Bruno Brito; Schlindwein,Gilson; Granada,Camille Eichelberger; Giongo,Adriana; Beneduzi,Anelise; Passaglia,Luciane Maria Pereira.
In the last decades, the use of plant growth-promoting rhizobacteria has become an alternative to improve crop production. Rhizobium leguminosarum biovar trifolii is one of the most promising rhizobacteria and is even used with non-legume plants. This study investigated in vitro the occurrence of plant growth-promoting characteristics in several indigenous R. leguminosarum biovar trifolii isolated from soils in the State of Rio Grande do Sul, Brazil. Isolates were obtained at 11 locations and evaluated for indoleacetic acid and siderophore production and inorganic phosphate solubilization. Ten isolates were also molecularly characterized and tested for antagonism against a phytopathogenic fungus and for plant growth promotion of rice seedlings. Of a total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizobacteria; Siderophore; Indoleacetic acid; Phosphate solubilization; Antagonism; Rice.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000500016
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Resistance to Anticarsia gemmatalis Hübner (Lepidoptera, Noctuidae) in transgenic soybean (Glycine max (L.) Merrill Fabales, Fabaceae) cultivar IAS5 expressing a modified Cry1Ac endotoxin Genet. Mol. Biol.
Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Pasquali,Giancarlo; Zaidi,Mohsin Abbas; Altosaar,Illimar; Bodanese-Zanettini,Maria Helena.
Somatic embryos of the commercial soybean (Glycine max) cultivar IAS5 were co-transformed using particle bombardment with a synthetic form of the Bacillus thuringiensis delta-endotoxin crystal protein gene cry1Ac, the beta-glucuronidase reporter gene gusA and the hygromycin resistance gene hpt. Hygromycin-resistant tissues were proliferated individually to give rise to nine sets of clones corresponding to independent transformation events. The co-bombardment resulted in a co-transformation efficiency of 44%. Many histodifferentiated embryos and 30 well-developed plants were obtained. Twenty of these plants flowered and fourteen set seeds. The integration and expression of the cry1Ac, gusA and hpt transgenes into the genomes of a sample of transformed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticarsia gemmatalis; Cry1Ac gene; IAS5 cultivar; Insect resistance; Transgenic soybean.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300020
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SOIL FUNGISTASIS AGAINST FUSARIUM GRAMINEARUM UNDER DIFFERENT CROP MANAGEMENT SYSTEMS Rev. Bras. Ciênc. Solo
Lisboa,Bruno Brito; Bayer,Cimélio; Passaglia,Luciane Maria Pereira; Camargo,Flávio Anastácio de Oliveira; Beneduzi,Anelise; Ambrosini,Adriana; Vargas,Luciano Kayser.
Soil management, in terms of tillage and cropping systems, strongly influences the biological properties of soil involved in the suppression of plant diseases. Fungistasis mediated by soil microbiota is an important component of disease-suppressive soils. We evaluated the influence of different management systems on fungistasis against Fusarium graminearum, the relationship of fungistasis to the bacterial profile of the soil, and the possible mechanisms involved in this process. Samples were taken from a long-term experiment set up in a Paleudult soil under conventional tillage or no-tillage management and three cropping systems: black oat (Avena strigose L.) + vetch (Vicia sativa L.)/maize (Zea mays L.) + cowpea (Vigna sinensis L.), black oat/maize, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crop systems; Fungistasis; No-tillage; Plant diseases.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000100069
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The combined analysis as the best strategy for Dual RNA-Seq mapping Genet. Mol. Biol.
Espindula,Eliandro; Sperb,Edilena Reis; Bach,Evelise; Passaglia,Luciane Maria Pereira.
Abstract In Dual RNA-Seq experiments the simultaneous extraction of RNA and analysis of gene expression data from both interacting organisms could be a challenge. One alternative is separating the reads during in silico data analysis. There are two main mapping methods used: sequential and combined. Here we present a combined approach in which the libraries were aligned to a concatenated genome to sort the reads before mapping them to the respective annotated genomes. A comparison of this method with the sequential analysis was performed. Two RNA-Seq libraries available in public databases consisting of a eukaryotic (Zea mays) and a prokaryotic (Herbaspirillum seropediceae) organisms were mixed to simulate a Dual RNA-Seq experiment. Libraries from real...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dual RNA-Seq; Sequential analysis; Combined analysis; Mapping strategies.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500803
Registros recuperados: 11
Primeira ... 1 ... Última
 

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