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| Registros recuperados: 13 | |
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| ROMAGNOLI, E. M.; MENDES, R.; DUNLAP, C.. |
| Fungi play important roles as decomposers, plant symbionts and pathogens in soil. While endophytes are microorganisms that dwell within plant tissues and have a symbiotic association with the host. The structures of fungal communities in the soil and in endophytic association are dependent up complex interactions with the environment and the host. These two communities have a great influence on plant health and development. Using culture-independent fungal community profiling, we investigated the effects of fertilizer (composted dairy solids + mustard seed meal) on fungal communities in soil and endophytic in a raspberry production system. During the study we evaluated the impact of primer selection ITS1 vs ITS2. We characterized the communities for both... |
| Tipo: Separatas |
Palavras-chave: Raspberry.; Fungo; Solo. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036663 |
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| ROMAGNOLI, E. M.. |
| Considerando a dieta como um fator modulador do microbioma ruminal, neste trabalho objetivou-se investigar o impacto do bagaço da cana-de-açúcar sobre a composição e funcionalidade das espécies microbianas residentes no rúmen de carneiros (Ovis aries). Foram utilizados seis animais machos fistulados de O. aries, dos quais três foram alimentados com uma dieta composta por 70% de volumoso e 30% de concentrado (tratamento controle) e outros três animais alimentados com uma dieta similar a anterior, mas com 14% do volumoso substituído por bagaço de cana-de-açúcar (tratamento bagaço). O conteúdo ruminal (líquido e fibra) foram amostrados quinzenalmente durante 60 dias. A partir dessas amostras foram acessadas a estrutura e a composição da comunidade microbiana... |
| Tipo: Teses |
Palavras-chave: Metagenômica; Metatranscriptômica 165 rRNA; Cana-de-açúcar.; Bagaço; Rúmen.; Metagenomics; Sugarcane bagasse. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065429 |
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| KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R.. |
| The rumen harbors complex microbial communities which participate in an efficient process to digest plant biomass. This ecosytem represents an untapped source of hydrolytic enzymes with potential application for second?generation biofuel production from lignocellulosic biomass. The search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to describe the functions and explores the potential for lignocellulolitic enzyme in the sheep rumen microbiome. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two... |
| Tipo: Separatas |
Palavras-chave: Enzima; Rúmen.. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1071445 |
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| KMIT, M. C. P.; LIMA, A. O. S.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R.. |
| Abstract: The lignocellulose present in the plant biomass, is a promising source of energy generation. However, the breakdown of plant biomass into simple sugars for bioethanol production is still inefficient and costly due to the recalcitrant nature of plant fiber. Sheep rumen microbiome is specialized in degradation of plant material, but most members of this complex community are non-cultivable in laboratory. Therefore, the search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to obtain plant biomass-degrading genes, selected from the... |
| Tipo: Separatas |
Palavras-chave: Lignocelluloytic enzymes; Secind generation biofuel; Microbial metagenome; Enzima; Biotecnologia; Metagenomics. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036656 |
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| MENDES, R.; ROMAGNOLI, E. M.; DUNLAP, C.; ABDALLA, A. L.. |
| Ruminants are herbivores and have evolved a symbiotic host-microbe relationship with a complex microbial community inhabiting the rumen allowing the use of lignocellulosic biomass as their main energy source. Considering that diet is one of the main drivers shaping the structure of the rumen microbiome, we investigated the impact of sugarcane bagasse in the rumen bacterial community dynamic using 16S rRNA (V3 and V6 regions) amplicon sequencing. We assessed three rumencannulated adult male sheep (Ovis aries) fed on a diet consisted of 30% concentrate and 70% roughage (control treatment) and three sheep fed on the same diet, but with 14% of the roughage portion replaced by sugarcane bagasse. Fluid and fiber were separately sampled 3 hours and 15, 30, 45,... |
| Tipo: Separatas |
Palavras-chave: Cana de açúcar; Bagaço; Bactéria; Rúmen.; Sugarcane bagasse.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1037517 |
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| Registros recuperados: 13 | |
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