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An efficient system for in vitro propagation of Bouchea fluminensis (Vell.) Mold. (Verbenaceae) Acta Botanica
Resende,Cristiano Ferrara de; Braga,Virgínia Fernandes; Silva,Cristiane Jovelina da; Pereira,Paula da Fonseca; Ribeiro,Cleberson; Salimena,Fátima Regina Gonçalves; Peixoto,Paulo Henrique Pereira.
This study aimed to establish and propagate in vitro plants of Bouchea fluminensis, a medicinal species known in Brazil as gervão-falso ("false verbena"), evaluating the influences of different growth regulators on in vitro multiplication and rooting stages, as well as examining ex vitro acclimatization of rooted plants. Explants were established on Murashige and Skoog medium at half strength of salts and vitamins without growth regulators. For multiplication, the explants were subjected to combinations of 6-benzyladenine (BA; 0, 2.5, 5.0 and 7.5 µM) and α-naphthalene-acetic acid (NAA; 0, 0.2, 0.4 and 0.6 µM). The medium found to induce the greatest number of shoot was that containing 5 µM of BA (NAA-free). For rooting, we evaluated three auxins (NAA,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; Biodiversity conservation; Espinhaço Mountain Range.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200005
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In vitro propagation and acclimatization of Lippia rotundifolia, an endemic species of Brazilian Campos Rupestres Rev. Ciênc. Agron.
Resende,Cristiano Ferrara de; Bianchetti,Ricardo Ernesto; Oliveira,Aline Mystica Silva de; Braga,Virgínia Fernandes; Peixoto,Paulo Henrique Pereira.
ABSTRACTThe importance in folk medicine, combined to threats in their environment, becomes necessary to carry out studies involving large-scale propagation of Lippia genus. Although the tissue culture propagation is widely disseminated for medicinal plants, for L. rotundifolia any article was published yet. The present study aimed to establish an efficient protocol for micropropagation of L. rotundifolia. Nodal segments, taken from plants collected in the Espinhaço Range, were disinfected, and cultures were initiated on MS medium with PVPP (1 g L- 1), sucrose (3%) and agar (0.7%). The culture were maintained in a growth room at controlled conditions. Disinfestation procedures and the supply of PVPP on culture media resulted in both reduced contamination...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; In vitro multiplication; In vitro rooting; Ex vitro acclimatization; Biodiversity conservation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902015000300582
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Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta) Acta Botanica
Silva-e-Costa,Juliana da Costa; Luizi-Ponzo,Andrea Pereira; Resende,Cristiano Ferrara de; Peixoto,Paulo Henrique Pereira.
ABSTRACT In bryophytes, establishment can occur by a sexual or asexual process, but the production of spores enables colonization of a wider range of habitats and substrates than can asexual propagules. Successful germination is critical for establishment in a new environment. This paper addresses germination and sporeling development in Frullania ericoides, a leafy liverwort species. Fresh spores were inoculated in vitro in different culture strengths of Knop’s nutrient solution (one-fourth strength, half strength, full strength, one and a half strength and double strength), in order to evaluate the effects of this solution on spore germination and on the development of external protonema. On the first assessment, spore germination was observed at all the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endosporic development; Knop’s medium; Liverworts; Nutrients; Protonema; Sporeling.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100019
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