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Registros recuperados: 4
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Analysis of the population structure of Uruguayan Creole cattle as inferred from milk major gene polymorphisms Genet. Mol. Biol.
Rincón,Gonzalo; Armstrong,Eileen; Postiglioni,Alicia.
The ancestors of Uruguayan Creole cattle were introduced by the Spanish conquerors in the XVII century, following which the population grew extensively and became semi-feral before the introduction of selected breeds. Today the Uruguayan Creole cattle genetic reserve consists of 575 animals. We used the tetra primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to analyze the kappa-casein, beta-casein, alphaS1-casein and alpha-lactoalbumin gene polymorphisms and restriction fragment length polymorphism PCR (RFLP-PCR) for the beta-lactoglobulin and the acylCoA:diacyl glycerol acyltransferase 1 (DGAT1) genes. The kappa-casein and beta-lactoglobulin genes presented very similar A and B allele frequencies, while the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DGAT1 gene; Milk protein; SNPs; Uruguayan Creole cattle.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300016
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Genomic variation and population structure detected by single nucleotide polymorphism arrays in Corriedale, Merino and Creole sheep Genet. Mol. Biol.
Grasso,Andrés N; Goldberg,Virginia; Navajas,Elly A; Iriarte,Wanda; Gimeno,Diego; Aguilar,Ignacio; Medrano,Juan F; Rincón,Gonzalo; Ciappesoni,Gabriel.
The aim of this study was to investigate the genetic diversity within and among three breeds of sheep: Corriedale, Merino and Creole. Sheep from the three breeds (Merino n = 110, Corriedale n = 108 and Creole n = 10) were genotyped using the Illumina Ovine SNP50 beadchip®. Genetic diversity was evaluated by comparing the minor allele frequency (MAF) among breeds. Population structure and genetic differentiation were assessed using STRUCTURE software, principal component analysis (PCA) and fixation index (F ST). Fixed markers (MAF = 0) that were different among breeds were identified as specific breed markers. Using a subset of 18,181 single nucleotide polymorphisms (SNPs), PCA and STUCTURE analysis were able to explain population stratification within...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allele frequencies breed composition; Fixation index; Ovine SNP50; Population stratification; Principal component analysis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300011
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Methylation-specific PCR analysis in Col8A1 promoter in Creole cattle carrier of rob(1;29) Electron. J. Biotechnol.
Postiglioni,Alicia; García,Cristina B; Rincón,Gonzalo; Arruga,M. Victoria.
Robertsonian translocation (rob(1;29)) is the most frequent structural chromosomal abnormality in cattle. Heterozygous carriers have a normal phenotype but show a 3-5% decrease in fertility. Chromatin decondensation was evaluated similar to the inactive X chromosome when submitted to demethylating agent. Based on this result, and the concept that imprinted genes are essential in embryonic development, we decided to query genes located on BTA1 and BTA29 that could undergo genome imprinting. The collagen typeVIII-α1 (Col8A1) acted on extracellular matrix structural proteins. DNA bisulfite conversion and sequentiation methods were used to compare its differential methylation patterns. It was performed on eight Creole cattle DNA blood samples from normal and...
Tipo: Journal article Palavras-chave: Bisulfite; Collagen; Creole cattle; Cytosines; Methylation.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300013
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Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus) Genet. Mol. Biol.
Armstrong,Eileen; Postiglioni,Alicia; Martínez,Amparo; Rincón,Gonzalo; Vega-Pla,José Luis.
The Uruguayan Creole cattle genetic reserve consists of a herd of about 600 animals (bulls, cows and calves) located in an indigenous habitat of 650 hectares. In a previous study, a random sample from this herd showed high heterozygosity and a Hardy-Weinberg equilibrium for markers of major genes related to milk production. To study its genetic diversity we genotyped a sample of bulls (N = 19 out of 23 for the whole herd) using the PCR reaction with a set of 17 microsatellite markers. Between two and seven different alleles were identified per microsatellite in a total of 73 alleles. The expected mean heterozygosity (He) per locus was between 0.465 and 0.801, except for microsatellite HEL13 which gave a He value of 0.288. The expected mean heterozygosity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Uruguayan Creole cattle; Genetic diversity; Microsatellites.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200012
Registros recuperados: 4
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