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| Pereira,Wendell Jacinto; Melo,Arthur Tavares de Oliveira; Coelho,Alexandre Siqueira Guedes; Rodrigues,Fabiana Aparecida; Mamidi,Sujan; Alencar,Sérgio Amorim de; Lanna,Anna Cristina; Valdisser,Paula Arielle Mendes Ribeiro; Brondani,Claudio; Nascimento-Júnior,Ivanildo Ramalho do; Borba,Tereza Cristina de Oliveira; Vianello,Rosana Pereira. |
| Abstract Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Water deficit; Functional genomics; Single nucleotide polymorphism (SNP). |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100309 |
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| Rodrigues,Fabiana Aparecida; Marcolino-Gomes,Juliana; Carvalho,Josirlei de Fátima Corrêa; Nascimento,Leandro Costa do; Neumaier,Norman; Farias,José Renato Bouças; Carazzolle,Marcelo Falsarella; Marcelino,Francismar Corrêa; Nepomuceno,Alexandre Lima. |
| Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Drought; Transcriptome. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011 |
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