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Evaluation of the Resistance of Transgenic C5 Plum (Prunus domestica L.) against Four Chilean Plum Pox Virus Isolates through Micro-Grafting Chilean J. Agric. Res.
Wong,Wendy; Barba,Paola; Álvarez,Catalina; Castro,Álvaro; Acuña,Manuel; Zamora,Pablo; Rosales,Marlene; Dell´Orto,Paola; Moynihan,Michael R; Scorza,Ralph; Prieto,Humberto.
The transgenic plum (Prunus domestica L.) C5, in which the coat protein (CP) gene of the Plum pox virus (PPV) is inserted, represents a unique example of the use of genetic engineering for fruit crop improvement in Prunus spp. Field trials in Poland, Romania, and Spain have demonstrated resistance of C5 to several D and M strain PPV isolates. In Chile, the quarantine regulations for PPV and for genetically modified (GM) plants require that the testing of C5 for resistance to Chilean PPV isolates be done under controlled isolated conditions. To carry out these tests C5 shoots were multiplied in vitro and micro-grafted onto four Adesoto101 (Prunus insititia L.) rootstock populations that had been previously infected each with one of four Chilean PPV-Ds....
Tipo: Journal article Palavras-chave: C5; Chilean isolates; Micro-grafting.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000300004
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Variation over time in the prevalences of three viruses of the grapevine leafroll complex in a commercial vineyard in south-central Chile Ciencia e Investigación Agraria
Mujica,María Valentina; Mora,Roxana; Rosales,Marlene; Sandoval,Claudio.
A vineyard in south-central Chile was surveyed during five growing seasons to analyze the increase over time in the number of plants infected with Grapevine leafroll-associated virus-1, -2 and -3. At the end of the study, GLRaV-3 was found to be the most prevalent virus, with 46% of plants infected by this pathogen. GLRaV-2 infected 13% of plants, and GLRaV-1 infected 12%. Considering all three viruses together, the prevalence of infection increased exponentially from 14% of the plants at the beginning of the study period to 68% at the end. The patterns of the spread of GLRaV-2 and GLRaV-3 were mostly along the rows, whereas the pattern of spread for GLRaV-1 could not be determined.
Tipo: Journal article Palavras-chave: Field transmission; Grapevine leafroll-associated virus; GLRaV-1; GLRaV-2; GLRaV-3; Virus diagnosis.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000100012
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