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Challenges in proteome analyses of tropical plants Braz. J. Plant Physiol.
Balbuena,Tiago S.; Dias,Leonardo L. C.; Martins,Mariana L. B.; Chiquieri,Tatiana B.; Santa-Catarina,Claudete; Floh,Eny I. S.; Silveira,Vanildo.
Genome sequencing of various organisms allow global analysis of gene expression, providing numerous clues on the biological function and involvement in the biological processes studied. Proteomics is a branch of molecular biology and biotechnology that has undergone considerable development in the post-genomic era. Despite the recent significant advancements in proteomics techniques, still there is much to be improved. Due to peculiarities to the plant kingdom, proteomics approaches require adaptations, so as to improve efficiency and accuracy of results in plants. Data generated by proteomics can substantially contribute to the understanding and monitoring of plant physiological events and development of biotechnological strategies. Especially for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-DE; Mass spectrometry; Recalcitrant species; Proteomics; Unknown genome.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200001
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Contribution of dark septate fungi to the nutrient uptake and growth of rice plants BJM
Vergara,Carlos; Araujo,Karla Emanuelle Campos; Alves,Luiziene Soares; Souza,Sônia Regina de; Santos,Leandro Azevedo; Santa-Catarina,Claudete; Silva,Krisle da; Pereira,Gilmara Maria Duarte; Xavier,Gustavo Ribeiro; Zilli,Jerri Édson.
ABSTRACT The use of dark septate fungi (DSE) to promote plant growth can be beneficial to agriculture, and these organisms are important allies in the search for sustainable agriculture practices. This study investigates the contribution of dark septate fungi to the absorption of nutrients by rice plants and their ensuing growth. Four dark septate fungi isolates that were identified by Internal transcribed spacer phylogeny were inoculated in rice seeds (Cv. Piauí). The resulting root colonization was estimated and the kinetic parameters Vmax and Km were calculated from the nitrate contents of the nutrient solution. The macronutrient levels in the shoots, and the NO3--N, NH4+-N, free amino-N and soluble sugars in the roots, sheathes and leaves were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza sativa L.; DSE; NO3−-N; Tillering; Colonization.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100067
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In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae) Acta Botanica
Balbuena,Tiago Santana; Santa-Catarina,Claudete; Silveira,Vanildo; Kato,Massuo Jorge; Floh,Eny Iochevet Segal.
Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l-1) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l-1 2,4-D and 2 mg.l-1 BA supplemented medium, and in the absence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Callogenesis; Cell suspension culture; Morphogenesis; Pariparoba; Plant growth regulators.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062009000100029
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Micropropagação do porta-enxerto de macieira 'Seleção 69' tolerante à podridão do colo (Phytophthora cactorum) Ciência Rural
Santa-Catarina,Claudete; Maciel,Scheila da Conceição; Denardi,Frederico; Pedrotti,Enio Luiz.
A utilização de porta-enxertos anões, tolerantes a problemas fitossanitários da cultura da macieira, como a podridão do colo (Phytophthora cactorum), pode aumentar o rendimento e reduzir os custos de produção. Com o presente trabalho, objetivou-se estabelecer um protocolo de micropropagação do porta-enxerto 'Seleção 69', tolerante à podridão do colo. Ápices meristemáticos foram cultivados in vitro em meio de cultura MS, suplementado com 4,4mM de 6-Benzilaminopurina (BAP), 2,5mM de Ácido indolbutírico (AIB), 0,3mM de Ácido giberélico (GA3), 3% de sacarose e 0,6% de ágar. Foram testados o efeito da concentração de BAP (2,2 e 4,4mM) e o efeito dos meios de cultura (MS, Cheng e Quoirin & Lepoivre) na multiplicação das brotações. No alongamento das...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ápices meristemáticos; Cultura in vitro; Enraizamento ex vitro; Aclimatização.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000500003
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Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures BABT
Carone,Sílvia Bicudo; Santa-Catarina,Claudete; Silveira,Vanildo; Floh,Eny Iochevet Segal.
The aim of this work was to determine PAs levels in pith tissues and callus cultures from haploid and diploid tobacco plants, explanted from the apical and basal regions of the stem. These explants were cultured in an RM-64 medium supplied with IAA and kinetin, under light or in the dark, during successive subcultures. PAs levels followed a basipetal decrease in diploid and an increase in haploid, pith tissues. A similar pattern of total PAs (free + conjugated) was observed for the callus of diploid and haploid plants maintained in the light, and for the haploid callus in the dark, whereas the diploid callus in the dark showed a constant increase in total PAs levels until the end of culture. The PA increase in the diploid callus in the dark was related to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechnology; Nicotiana tabacum; Ploidy; Putrescine; Spermidine; Spermine.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200020
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Two-dimensional gel electrophoretic protein profile analysis during seed development of Ocotea catharinensis: a recalcitrant seed species Braz. J. Plant Physiol.
Dias,Leonardo L.C.; Balbuena,Tiago S.; Silveira,Vanildo; Santa-Catarina,Claudete; Schevchenko,Andrej; Floh,Eny I. S..
The aim of the present work was to characterize changes in the protein profile throughout seed development in O. catharinensis, a recalcitrant species, by two-dimensional gel electrophoresis. Protein extraction was undertaken by using a thiourea/urea buffer, followed by a precipitation step with 10% TCA. Comparative analysis during seed development showed that a large number of proteins were exclusively detected in each developmental stage. The cotyledonary stage, which represents the transition phase between embryogenesis and the beginning of metabolism related to maturation, presents the highest number of stage-specific spots. Protein identification, through MS/MS analysis, resulted in the identification of proteins mainly related to oxidative metabolism...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-DE; Comparative proteomics; Embryo development; Storage protein; Lauraceae.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000100003
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