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A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol BABT
Anschau,Andréia; Santos,Lucielen Oliveira dos; Alegre,Ranulfo Monte.
This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respectively. Further studies done in 5 L bioreactor resulted 241.3 mg/L GSH after 96 h in batch fermentation without amino acids addition and the concentration of biomass was 12.1 g/L. In batch fermentation with the addition of the three amino acids (4 mM cysteine, glycine and glutamic acid at 32 h), biomass reached to 25 g/L and GSH, 236.1 mg/L at 96 h of fermentation. The strategy of precursor amino acids...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione; Fermentation; Fed Batch Culture; Amino Acids; Response Surface Methodology.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500017
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Glutathione production using magnetic fields generated by magnets BABT
Santos,Lucielen Oliveira dos; Gonzales,Tatiane Araujo; Úbeda,Beatriz Torsani; Alegre,Ranulfo Monte.
The objective of this work was to study the production of GSH by Saccharomyces cerevisiae ATCC 7754 in a fermentor (5 L) using a cell recycle system with magnets. The fermentation conditions were 20°C, 500 rpm, 5% (v/v) of inoculum, pHinitial 5, 1.1 vvm aeration and total fermentation time of 72 h. The time of application of MF ranged from 24, 48 or 72 h. In comparison to the control experiment, the best results were obtained with 72 h of application of MF. The cell concentration reached 19.5 g/L and GSH concentration was 271.9 mg/L that corresponded to an increase of 2.63 and 32.1% compared to the control experiment, respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione; Magnetic field; Saccharomyces cerevisiae; Batch fermentation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600016
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