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| Schilichting,C.L.R.; Lima,K.C.M.; Cestari Junior,L.A.; Sekiyama,J.Y.; Silva,F.M.; Milani,H.. |
| A gravimetric method was evaluated as a simple, sensitive, reproducible, low-cost alternative to quantify the extent of brain infarct after occlusion of the medial cerebral artery in rats. In ether-anesthetized rats, the left medial cerebral artery was occluded for 1, 1.5 or 2 h by inserting a 4-0 nylon monofilament suture into the internal carotid artery. Twenty-four hours later, the brains were processed for histochemical triphenyltetrazolium chloride (TTC) staining and quantitation of the schemic infarct. In each TTC-stained brain section, the ischemic tissue was dissected with a scalpel and fixed in 10% formalin at 0ºC until its total mass could be estimated. The mass (mg) of the ischemic tissue was weighed on an analytical balance and compared to its... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Focal cerebral ischemia; Infarct size; Rats; Triphenyltetrazolium chloride stain; Weighing method. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400008 |
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