|
|
|
|
|
| Silva,Jorge A.G. da. |
| Expressed sequence tags (ESTs) in the sugarcane (Saccharum spp) database (SUCEST) were electronically searched and 402 microsatellites identified. Various dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were found, with these being more frequently observed in ESTs obtained from flower cDNA libraries. PCR primers were designed for 20 of these SSRs and were tested on eight sugarcane genotypes, the sequences of these primers and a list of known sugarcane genes containing SSR motifs being presented in this paper. Polymorphisms were evident both at the cultivar level and between Saccharum species. These results show that EST-derived SSRs in Saccharum species are useful because they are polymorphic and transferable. The large number of... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100021 |
| |
|
|
| Silva,Marcelo de A.; Jifon,John L.; Silva,Jorge A.G. da; Sharma,Vivek. |
| Drought is one of the major limitations to plant productivity worldwide. Identifying suitable screening tools and quantifiable traits would facilitate the crop improvement process for drought tolerance. In the present study, we evaluated the ability of four relatively physiological parameters (variable-to-maximum chlorophyll a fluorescence ratio, F v/F m; estimated leaf chlorophyll content via SPAD index; leaf temperature, LT; and, leaf relative water content, RWC) to distinguish between drought tolerant and susceptible sugarcane genotypes subjected to a 90-d drought cycle. Eight field-grown genotypes were studied. By 45 d after the onset of treatments, the F v/F m, SPAD index and RWC of drought-stressed plants had declined significantly in all genotypes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chlorophyll a fluorescence; Relative water content; Leaf temperature; Saccharum; Water deficit. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000300003 |
| |
|
|
|
