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Registros recuperados: 9
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An alkaline single-cell gel electrophoresis (comet) assay for environmental biomonitoring with native rodents Genet. Mol. Biol.
Silva,Juliana da; Freitas,Thales R.O. de; Marinho,Jorge R.; Speit,Günter; Erdtmann,Bernardo.
The main advantages of single-cell gel electrophoresis (SCG) are its applicability to any eukaryotic organism and cell type, its low cost and the short time required to obtain results. These properties make the SCG assay particularly useful in screening for environmental genotoxicity. The present study describes a modified version of this technique for use in field work with native rodents and examines some factors which influence the outcome of the assay. Wild rodents (Ctenomys torquatus, "tuco-tuco") from a region close to a strip coal mine and from a region with no coal mines were used. Animals from the coal mining region had significantly more DNA damage than those from the control area. The use of this SCG technique for direct sampling in the field...
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100042
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Association between cervical lesion grade and micronucleus frequency in the Papanicolaou test Genet. Mol. Biol.
Bueno,Caroline Tanski; Silva,Cláudia Maria Dornelles da; Barcellos,Regina Bones; Silva,Juliana da; Santos,Carla Rossana dos; Menezes,João Evangelista Sampaio; Menezes,Honório Sampaio; Rossetti,Maria Lucia Rosa.
The aim of this study was to evaluate the association between the frequency of micronuclei (MN) and the cellular changes detected in the conventional Papanicolaou test. One hundred and seventy-four Papanicolaou test smears with cellular changes were examined. MN screening was done in cytopathological smears by counting 1,000 cervical cells in a light microscope. MN frequencies were significantly higher in the group with cellular changes compared to the control group (p < 0.001). The mean MN frequencies were 0.95 ± 1.12 (mean ± SD) in the control group (n = 223), 2.98 ± 1.20 in individuals with atypical squamous cells of undetermined significance (ASC-US) (n = 50), 4.04 ± 1.45 in cervical intraepithelial neoplasia (CIN) I (n = 52), 5.97 ± 1.83 in CIN II...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cervical cancer; Cervical lesions; Micronucleus; Papanicolaou test.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400004
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DNA damage in children and adolescents with cardiovascular disease risk factors Anais da ABC (AABC)
Kliemann,Mariele; Prá,Daniel; Müller,Luiza L.; Hermes,Liziane; Horta,Jorge A.; Reckziegel,Miriam B.; Burgos,Miria S.; Maluf,Sharbel W.; Franke,Silvia I.R.; Silva,Juliana da.
The risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cardiovascular diseases; Micronucleus test; Comet assay; Diet; Anthropometry; Child; Adolescent.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300025
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Effects of artichoke (Cynara scolymus) leaf and bloom head extracts on chemically induced DNA lesions in Drosophila melanogaster Genet. Mol. Biol.
Jacociunas,Laura Vicedo; Dihl,Rafael Rodrigues; Lehmann,Mauricio; Ferraz,Alexandre de Barros Falcão; Richter,Marc François; Silva,Juliana da; Andrade,Heloísa Helena Rodrigues de.
The genotoxicity of bloom head (BHE) and leaf (LE) extracts from artichoke (Cynara scolymus L.), and their ability to modulate the mutagenicity and recombinogenicity of two alkylating agents (ethyl methanesulfonate - EMS and mitomycin C - MMC) and the intercalating agent bleomycin (BLM), were examined using the somatic mutation and recombination test (SMART) in Drosophila melanogaster. Neither the mutagenicity nor the recombinogenicity of BLM or MMC was modified by co- or post-treatment with BHE or LE. In contrast, co-treatment with BHE significantly enhanced the EMS-induced genotoxicity involving mutagenic and/or recombinant events. Co-treatment with LE did not alter the genotoxicity of EMS whereas post-treatment with the highest dose of LE significantly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artichoke; Drosophila melanogaster; Recombinogenicity; SMART.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100015
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Feedlot performance, feeding behavior and rumen morphometrics of Nellore cattle submitted to different feeding frequencies Scientia Agricola
Silva,Juliana da; Carrara,Tássia Veluma Barbosa; Pereira,Murillo Ceola Stefano; Oliveira,Cassiele Aparecida de; Batista Júnior,Ivan Carlos; Watanabe,Daniel Hideki Mariano; Rigueiro,André Luiz Nagatani; Arrigoni,Mário De Beni; Millen,Danilo Domingues.
ABSTRACT: Owing to the lack of information in the literature about the ideal feeding frequency adopted by feedlot systems, this study was designed to evaluate the effect of different feeding frequencies on performance, carcass traits, feeding behavior and rumen morphometrics of Nellore cattle. Forty-eight yearling bulls were housed in individual pens with an average initial body weight of 358.2 kg. The experiment was designed as a completely randomized block and the treatments were the feeding of cattle one, two, three, or four times daily (n = 12 per treatment). Orthogonal contrasts were used to assess linear, quadratic and cubic relationship between feeding frequency and the dependent variable. As feeding frequency increased, final body weight (p = 0.03)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zebu; Carcass; Concentrate; Intake; Papillae.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000200121
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Genotoxicity of Nicotiana tabacum leaves on Helix aspersa Genet. Mol. Biol.
Silva,Fernanda R. da; Erdtmann,Bernardo; Dalpiaz,Tiago; Nunes,Emilene; Ferraz,Alexandre; Martins,Tales L.C.; Dias,Johny F.; Rosa,Darlan P. da; Porawskie,Marilene; Bona,Silvia; Silva,Juliana da.
Tobacco farmers are routinely exposed to complex mixtures of inorganic and organic chemicals present in tobacco leaves. In this study, we examined the genotoxicity of tobacco leaves in the snail Helix aspersa as a measure of the risk to human health. DNA damage was evaluated using the micronucleus test and the Comet assay and the concentration of cytochrome P450 enzymes was estimated. Two groups of snails were studied: one fed on tobacco leaves and one fed on lettuce (Lactuca sativa L) leaves (control group). All of the snails received leaves (tobacco and lettuce leaves were the only food provided) and water ad libitum. Hemolymph cells were collected after 0, 24, 48 and 72 h. The Comet assay and micronucleus test showed that exposure to tobacco leaves for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Comet assay; Genotoxicity; Micronucleus test; Helix aspersa; Nicotiana tabacum; Tobacco leaves.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200018
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Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus Genet. Mol. Biol.
Pereira,Mayra Ramos de Jesus; Pinhatti,Valéria Rodrigues; Silveira,Maiele Dornelles da; Matzenbacher,Cristina Araujo; Freitas,Thales Renato Ochotorena de; Silva,Juliana da; Camassola,Melissa; Nardi,Nance Beyer.
Abstract Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil. Cultures showed typical morphology and proliferation potential. Adipose-derived MSCs showed trilineage potential. Cultures derived from adipose tissue, bone marrow and brain were immunophenotyped with negative results for CD31, CD44, CD45,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesenchymal stem/stromal cells; Tuco-tuco; Ctenomys minutus.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500870
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Susceptibility to DNA damage in workers occupationally exposed to pesticides, to tannery chemicals and to coal dust during mining Genet. Mol. Biol.
Kvitko,Katia; Bandinelli,Eliane; Henriques,João A.P.; Heuser,Vanina D.; Rohr,Paula; Silva,Fernanda R. da; Schneider,Naye Balzan; Fernandes,Simone; Ancines,Camile; Silva,Juliana da.
Our mutagenesis group has been studying with important economic drivers of our state, such as agriculture, the footwear and leather industry and open-cast coal mining. Working conditions in these sectors have potentially harmful to humans. The aim of these studies is to determine the health risk of workers by biomonitoring subjects exposed to genotoxic agents. The main results of our studies with vineyard farmers we observed a high rate of MN and DNA damage in individuals exposed to pesticides (p < 0.001). In addition, some effects of genetic polymorphisms in the modulation of MN results were observed in this group. Tobacco farmers were also evaluated at different crop times. The results showed a significant increase in the Damage index and frequency in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Occupational exposure; Comet assay; Micronucleus test; Pesticide; Coal dust.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600022
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Unexpected chromosomal alterations in Tayassu tajacu (Artiodactyla: Tayassuidae) in captivity Braz. J. Vet. Res. Anim. Sci.
Lima,José Fernando de Sousa; Guedes,Fátima Becker; Silva,Roxane Wirschum; Hass,Iris; Cavalli,Iglenir João; Silva,Juliana da; Freitas,Thales Renato de; Sbalqueiro,Ives José.
Wild animals have been used as bioindicators in situations in which the environment was exposed to chemical agents. In general, chemical agents may induce chromosomal aberrations, such as breaks and gaps. The peccary, Tayassu tajacu is a pig relative that exhibits a very stable karyotype with the only described alterations being of the form of the X chromosome. Chromosomal gaps and breaks were observed at high frequencies during cytogenetics analyses. These alterations were observed in the chromosomes autossomics. Reviews of the literature and of the data described herein suggests that an vermifuge, the ivermectin base, was the most likely cause of these chromosomal alterations.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tayassu; Karyotype; Chromosome; Chromosomal alterations.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000100002
Registros recuperados: 9
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