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Silva,Roberto do Nascimento; Monteiro,Valdirene Neves; Alcanfor,Joana D'Arc Ximenes; Assis,Elaine Meire; Asquieri,Eduardo Ramirez. |
Este trabalho teve como objetivo comparar vários métodos para a determinação de açúcares redutores (A.R) e totais (A.T) em mel, a fim de suprir carências dos laboratórios de análises de alimentos das indústrias alimentícias e adaptar o método de ADNS (Ácido 3,5-Dinitrossalicílico) para análise de açúcares totais. Foram utilizadas quinze amostras de méis procedentes de diferentes cidades do estado de Goiás. Foram utilizados os seguintes métodos: Antrona, ADNS, complexometria com EDTA (Ácido etilenodiaminotetracético), Cromatografia em Camada Delgada, Fenol Sulfúrico, Lane & Eynon, Luff-Schoorl, Munson-Walker, Refratometria na escala Brix e Somogyi-Nelson. Para açúcares redutores os métodos Luff-School e Munson-Walker foram significativamente... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mel; Açúcares redutores e totais; Métodos. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612003000300007 |
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Martins-Santana,Leonardo; Paula,Renato Graciano de; Silva,Adriano Gomes; Lopes,Douglas Christian Borges; Silva,Roberto do Nascimento; Silva-Rocha,Rafael. |
Abstract Trichoderma reesei is the main filamentous fungus used in industry to produce cellulases. Here we investigated the role of CRZ1 and Ca2+signaling in the fungus T. reesei QM6a concerning holocellulases production. For this, we first searched for potential CRZ1 binding sites in promoter regions of key genes coding holocellulases, as well as transcriptional regulators and sugar and calcium transporters. Using a nearly constructed T. reeseiAcrz1 strain, we demonstrated that most of the genes expected to be regulated by CRZ1 were affected in the mutant strain induced with sugarcane bagasse (SCB) and cellulose. In particular, our data demonstrate that Ca2+ acts synergistically with CRZ1 to modulate gene expression, but also exerts CRZ1-independent... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene regulatory network; Cellulase expression; Transcriptional regulation; Calcium sensing. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400401 |
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Silva,Roberto do Nascimento; Quintino,Fábio Pereira; Monteiro,Valdirene Neves; Asquieri,Eduardo Ramirez. |
The high demands for sugars and the development of enzymatic technology have increased the production of sweeteners, especially for glucose and fructose syrups. This work describe a technology for glucose and fructose syrups from Brazilian cassava starch using enzymes produced by soil microrganisms isolated from the Brazilian Cerrado soil. Firstly, Aspergillus niger and Streptomyces sp. were isolated from the soil and used as glucoamylase (GA) and glucose isomerase (GI) producer sources. After characterization, GA and GI exhibited optimum pH 4.5 and 8.0, respectively. GA showed maximum activity at 60 ºC and GI at 85 ºC. GA and GI retained 65 and 80%, respectively, of initial activity after 180 minutes of incubation at 60 ºC. The kinetic parameters Km and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glucoamylase; Glucose isomerase; Glucose-fructose syrups; Cassava. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000100031 |
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