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Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential R. Bras. Zootec.
Maia,Natália da Costa; Souza,Patrícia Nirlane da Costa; Godinho,Bárbara Temponi Vilarino; Moreira,Silvino Intra; Abreu,Lucas Magalhães de; Jank,Liana; Cardoso,Patrícia Gomes.
ABSTRACT This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Grasses; Molecular identification; Plant pathogens; RDNA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100303
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Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis) R. Bras. Zootec.
Timbó,Ana Luiza de Oliveira; Souza,Patrícia Nirlane da Costa; Pereira,Roselaine Cristina; Nunes,Juliane Dornellas; Pinto,José Eduardo Brasil Pereira; Souza Sobrinho,Fausto de; Davide,Lisete Chamma.
The objective of the present work was to induce chromosome doubling in genotypes of Brachiaria ruziziensis with potential for production of good-quality biomass and tolerance/resistance to stress caused by aluminum and spittlebugs. Germinated seeds of B. ruziziensis were immersed in 0.1% colchicine solution for 2 or 3 hours. Ploidy level was determined by flow cytometry and confirmed by chromosome count. Fertility of the tetraploid plants was assessed through evaluation of pollen viability by staining. There was no statistical difference with regard to seedling survival and number of duplicate seedlings between the treatments. Survival rate was 8%, of which 11.45% were tetraploid genotypes. The mean viability of pollen grains from the evaluated tetraploid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA content; Forage plant breeding; Number of chromosomes; Polyploidy induction; Pollen viability.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300004
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