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Registros recuperados: 6
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Analysis of expressed sequence tags from Citrus sinensis L. Osbeck infected with Xylella fastidiosa Genet. Mol. Biol.
Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Targon,Maria Luisa P.N.; Carlos,Eduardo F.; Locali-Fabris,Eliane C.; Amaral,Alexandre M.; Freitas-Astúa,Juliana; Silva-Pinhati,Ana Carla O.; Boscariol-Camargo,Raquel L.; Berger,Irving J.; Rodrigues,Carolina M.; Reis,Marcelo S.; Machado,Marcos A..
In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC) symptoms, we adopted a strategy of comparing two EST libraries from sweet orange [Citrus sinensis (L.) Osbeck]. One of them was prepared with plants showing typical CVC symptoms caused by Xylella fastidiosa and the other with non-inoculated plants. We obtained 15,944 ESTs by sequencing the two cDNA libraries. Using an in silico hybridization strategy, 37 genes were found to have significant variation at the transcriptional level. Within this subset, 21 were up-regulated and 16 were down-regulated in plants with CVC. The main functional categories of the down-regulated transcripts in plants with CVC were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sweet orange; EST; CVC; Compatible interaction.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500023
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Bioinformatics for the Citrus EST Project (CitEST) Genet. Mol. Biol.
Reis,Marcelo S.; Takita,Marco A.; Palmieri,Darío A.; Machado,Marcos A..
In this work we describe all the computational environments, pipelines, and web services developed for the CitEST transcriptome project, on which all the annotation researchers relied. We also present a complete list of CitEST libraries and, for each of them, the general features after the in silico processing, showing some quantitative information.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transcriptomics; Comparative genomics; Clustering; Trimming; Digital northern.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500031
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Comparative analysis of differentially expressed sequence tags of sweet orange and mandarin infected with Xylella fastidiosa Genet. Mol. Biol.
Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Campos,Magnólia A.; Teixeira,Juliana E.C.; Targon,Maria Luísa P.N.; Carlos,Eduardo F.; Ravasi,Juliano F.; Fischer,Carlos N.; Machado,Marcos A..
The Citrus ESTs Sequencing Project (CitEST) conducted at Centro APTA Citros Sylvio Moreira/IAC has identified and catalogued ESTs representing a set of citrus genes expressed under relevant stress responses, including diseases such as citrus variegated chlorosis (CVC), caused by Xylella fastidiosa. All sweet orange (Citrus sinensis L. Osb.) varieties are susceptible to X. fastidiosa. On the other hand, mandarins (C. reticulata Blanco) are considered tolerant or resistant to the disease, although the bacterium can be sporadically detected within the trees, but no disease symptoms or economic losses are observed. To study their genetic responses to the presence of X. fastidiosa, we have compared EST libraries of leaf tissue of sweet orange Pêra IAC (highly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ESTs; Citrus variegated chlorosis; Biotic stress; CitEST; Phytopathogen.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500024
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Differential expression of genes identified from Poncirus trifoliata tissue inoculated with CTV through EST analysis and in silico hybridization Genet. Mol. Biol.
Cristofani-Yaly,Mariângela; Berger,Irving J.; Targon,Maria Luisa P.N.; Takita,Marco A.; Dorta,Sílvia de O.; Freitas-Astúa,Juliana; Souza,Alessandra A. de; Boscariol-Camargo,Raquel L.; Reis,Marcelo S.; Machado,Marcos A..
Citrus is the most important fruit crop in Brazil and Citrus tristeza virus (CTV) is considered one of the most important pathogens of citrus. Most citrus species and varieties are susceptible to CTV infection. However, Poncirus trifoliata, a close relative of citrus, is resistant to the virus. In order to better understand the responses of citrus plants to the infection of CTV, we constructed expressed sequence tag (EST) libraries with tissues collected from Poncirus trifoliata plants, inoculated or not with Citrus tristeza virus at 90 days after inoculation, grafted on Rangpur lime rootstocks. We generated 17,867 sequence tags from Poncirus trifoliata inoculated (8,926 reads) and not (8,941 reads) with a severe CTV isolate. A total of 2,782 TCs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus; Disease resistance; Citrus tristeza virus; Biotic stress.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500025
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In silico analysis of ESTs from roots of Rangpur lime (Citrus limonia Osbeck) under water stress Genet. Mol. Biol.
Boscariol-Camargo,Raquel L.; Berger,Irving J.; Souza,Alessandra A.; Amaral,Alexandre M. do; Carlos,Eduardo F.; Freitas-Astúa,Juliana; Takita,Marco A.; Targon,Maria Luisa P.N.; Medina,Camilo L.; Reis,Marcelo S.; Machado,Marcos A..
CitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Expressed sequence tags; Transcription; Rootstock; Drought; Molecular response.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500019
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Terpene production in the peel of sweet orange fruits Genet. Mol. Biol.
Takita,Marco A.; Berger,Irving J.; Basílio-Palmieri,Ana Carolina; Borges,Kleber M.; Souza,Juliana M. de; Targon,Maria L.N.P..
Terpenoids constitute the largest and most diverse class of natural products. They are important factors for aroma and flavor, and their synthesis is basically done from two compounds: isopentenyl diphosphate and dimethylallyl diphosphate. Isopentenyl diphosphate is synthesized through two different pathways, one that occurs in the cytoplasm and one in the plastid. With the sequencing of ESTs from citrus, we were able to perform in silico analyses on the pathways that lead to the synthesis of terpenes as well as on the terpene synthases present in sweet orange. Moreover, expression analysis using real-time qPCR was performed to verify the expression pattern of a terpene synthase in plants. The results show that all the pathways for isopentenyl diphosphate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: EST; Fruit; Terpenoids; Orange; Essential oil.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500012
Registros recuperados: 6
Primeira ... 1 ... Última
 

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