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Comparison of High Performance Liquid Chromatography with Fluorescence Detector and with Tandem Mass Spectrometry methods for detection and quantification of Ochratoxin A in green and roasted coffee beans BABT
Bandeira,Raquel Duarte da Costa Cunha; Uekane,Thais Matsue; Cunha,Carolina Passos da; Rodrigues,Janaina Marques; la Cruz,Marcus Henrique Campino de; Godoy,Ronoel Luiz de Oliveira; Fioravante,Andreia de Lima.
Two analytical methods for the determination and confirmation of ochratoxin A (OTA) in green and roasted coffee samples were compared. Sample extraction and clean-up were based on liquid-liquid phase extraction and immunoaffinity column. The detection of OTA was carried out with the high performance liquid chromatography (HPLC) combined either with fluorescence detection (FLD), or positive electrospray ionization (ESI+) coupled with tandem mass spectrometry (MS-MS). The results obtained with the LC-ESI-MS/MS were specific and more sensitive, with the advantages in terms of unambiguous analyte identification, when compared with the HPLC-FLD.
Tipo: Info:eu-repo/semantics/article Palavras-chave: HPLC-FLD; LC-MS/MS; Green Coffee and Roasted Coffee.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600004
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