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Assessment of viability of sperm cells of Litopenaeus vannamei on cryopreservation BABT
Uberti,Marcela Fornari; Vieira,Felipe do Nascimento; Salência,Helena Ragibo; Vieira,Genyess da Silva; Vinatea,Luis Alejandro.
Aiming at assessing the cryopreservation potential of Litopenaeus vannamei sperm cells, 74 spermatophores were manually extracted from the sexually mature individuals. After the toxicity test to define the cryoprotectant concentration, suspensions of spermatic cells were cryopreserved in the groups in freezing solutions comprising different cryoprotectants such as dimethyl sulfoxide (DMSO) and ethylene glycol (EG) at 10% concentration. Each treatment was divided in subgroups for storage in liquid nitrogen during 0, 30, 60 and 90 days, in triplicate. After thawing at 25ºC for 40 seconds, cell viability in the suspensions was analyzed under the microscope in eosin-nigrosin stain and flow cytometry. There were no significant differences between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryogenics; Semen; Marine shrimp; Cell viability.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300010
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Carbon footprint in commercial cultivation of marine shrimp: a case study in southern Brazil R. Bras. Zootec.
Belettini,Frank; Seiffert,Walter Quadros; Lapa,Katt Regina; Vieira,Felipe do Nascimento; Santo,Carlos Manoel do Espírito; Arana,Luis Alejandro Vinatea.
ABSTRACT A life-cycle assessment was conducted during semi-intensive and super-intensive commercial cultivation of marine shrimp from December 2011 to June 2012, considering all phases from the preparation of the nursery to harvesting of the shrimp, to determine the carbon footprints of each process. Inputs and outputs associated to the production of 1 kg of shrimp were evaluated using the CML-1A baseline method, V3.01 EU25, with Software SimaPro® 8.0.2, to identify the factors of impact that are most relevant to the category global warming, measured in kg of CO2 eq. The grow-out phase contributed the most to the final results in super-intensive culture, which had a higher carbon footprint, 47.9967 kg of CO2 eq., which was 1.0042 kg of CO2 eq. in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Environmental impact; Life-cycle analysis; Litopenaeus vannamei; Semi-intensive culture; Super-intensive culture.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100102
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Combining a probiotic with organic salts presents synergistic in vitro inhibition against aquaculture bacterial pathogens BABT
Bolivar,Norha Constanza; Legarda,Esmeralda Chamorro; Seiffert,Walter Quadros; Andreatta,Edemar Roberto; Vieira,Felipe do Nascimento.
ABSTRACT This study aimed to evaluate 1) the in vitro effect of organic salts on the growth of the probiotic Lactobacillus plantarum and then 2) the combined use of a probiotic with organic salts on the in vitro inhibition of V. alginolyticus, A. hydrophila, E. coli, P. aeruginosa, and S. agalactiae. In vitro tests were performed with eight different organic salts, including butyrate, propionate, succinate, citrate, formate, fumarate, glutamate, and acetate, at two pH values (6.2 and 7.1) to determine their effect on the growth kinetics of L. plantarum. In addition, each organic salt was tested alone and in combination with L. plantarum to evaluate the inhibitory effect against the pathogenic bacteria noted above in either condition. Sodium citrate and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Sodium butyrate; Sodium propionate; Aquaculture pathogenic bacteria.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100415
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Cultivation of juvenile fat snook (Centropomus parallelus Poey, 1860) fed probiotic in laboratory conditions BABT
Barbosa,Moysés Cavichioli; Jatobá,Adolfo; Vieira,Felipe do Nascimento; Silva,Bruno Corrêa; Mourino,José Luiz Pedreira; Andreatta,Edemar Roberto; Seiffert,Walter Quadros; Cerqueira,Vinícius Ronzani.
The objective of this study was to investigate the growth of juvenile fat snook (Centropomus parallelus) in laboratory conditions when fed a diet supplemented with the probiotic Lactobacillus plantarum. Changes in the intestinal flora, haematological parameters and growth performance were assessed using 180 fishes (54.2 ± 13.4 g each). The fishes were subjected to two treatments divided into six cages: 1) feed supplemented with probiotic, and 2) feed without probiotic (control). The temperature, dissolved oxygen and salinity were maintained at 25 ± 1 ° C, 4.0 mg.L-1 and 33 ‰, respectively. After 10 weeks of culture, the fishes fed probiotic had reduced viable culturable heterotrophic bacteria and Vibrio spp. and increased lactic acid bacteria in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Hematology; Bacterial microbiota; Body index.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400020
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Dieta suplementada com prebiótico, probiótico e simbiótico no cultivo de camarões marinhos PAB
Bolívar Ramírez,Norha; Seiffert,Walter Quadros; Vieira,Felipe do Nascimento; Mouriño,José Luiz Pedreira; Jesus,Gabriel Fernandes Alves; Ferreira,Gabriela Soltes; Andreatta,Edemar Roberto.
O objetivo deste trabalho foi avaliar o uso de suplemento prebiótico, probiótico e simbiótico, na dieta de camarões marinhos (Litopenaeus vannamei) e seus efeitos sobre o crescimento, a microbiota intestinal, a resposta imune e a resistência ao desafio experimental com Vibrio alginolyticus. Foram utilizados quatro tratamentos: prebiótico inulina; probiótico Lactobacillus plantarum; simbiótico Lactobacillus plantarum + inulina; e controle. Os camarões foram distribuídos em 16 tanques de dez mil litros de água, povoados com 200 camarões cada, cultivados por seis semanas. Avaliaram-se a microbiologia do trato intestinal dos camarões e a reposta imune, antes e após o desafio com V. alginolyticus. A concentração de Vibrio spp. no trato digestório foi menor em...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Litopenaeus vannamei; Vibrio alginolyticus; Desafio experimental; Inulina; Resposta imune.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800018
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Effect of carbon source on morphology and histodifferentiation of Araucaria angustifolia embryogenic cultures BABT
Steiner,Neusa; Vieira,Felipe do Nascimento; Maldonado,Sara; Guerra,Miguel Pedro.
The aim of the present work was to establish in vitro conditions for the induction, stabilization and proliferation of embryogenic cultures of A. angustifolia. Pre-cotyledonary staged zygotic embryos inoculated BM medium supplemented with 5 µM 2,4- D, 2 µM BAP and Kin, and 3% maltose or sucrose resulted in 66.7% induction rate. The rate of induction of embryogenic cultures was affected by the carbon source, as well the multiplication and morphology of the embryogenic cultures. Embryogenic cultures maintained in BM medium with maltose presented bipolar morphology. Globular somatic embryos were obtained BM medium with 9% (PEG) and (9%) maltose. These results could establish an in vitro regenerative protocol towards the conservation and improvement of this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell competence; Somatic embryogenesis; Carbohydrate; Conifers.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800005
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Effect of dietary supplementation with butyrate and probiotic on the survival of Pacific white shrimp after challenge with Vibrio alginolyticus R. Bras. Zootec.
Bolívar Ramírez,Norha Constanza; Rodrigues,Marysol Santos; Guimarães,Ariane Martins; Guertler,Cristhiane; Rosa,Juliana Ribeiro; Seiffert,Walter Quadros; Andreatta,Edemar Roberto; Vieira,Felipe do Nascimento.
ABSTRACT This study evaluated the performance, immunology, and survival of the Pacific white shrimp Litopenaeus vannamei to experimental challenge to Vibrio alginolyticus based on the use of the probiotic Lactobacillus plantarum and the combined use of probiotic and butyrate. Four different diets resulted from the addition of additives: butyrate, probiotic, butyrate + probiotic, and control (no additives). The attractiveness of the diets was assessed by the percentage of positive choices and rejections, using a dual-choice Y-maze format aquarium. The shrimps were fed during four weeks and performance parameters, intestinal microbiota, and immunological parameters were all evaluated. Subsequently, the shrimps were challenged with V. alginolyticus and after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal production; Lactic acid; Organic acids.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000600471
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Effects of stocking density and artificial substrates on yield and water quality in a biofloc shrimp nursery culture R. Bras. Zootec.
Legarda,Esmeralda Chamorro; Barcelos,Suhellen Santos; Redig,Joselle Cursino; Ramírez,Norha Constanza Bolívar; Guimarães,Ariane Martins; Santo,Carlos Manoel do Espírito; Seiffert,Walter Quadros; Vieira,Felipe do Nascimento.
ABSTRACT The objective of this study was to evaluate the effect of different stocking densities and the presence/absence of two artificial substrates on water quality and production rates of marine shrimp in a biofloc shrimp nursery culture. Two experiments were performed: different stocking densities with mosquito netting substrate and the presence/absence of polyester-type substrate. The first experiment lasted 38 days, and shrimp at an initial weight of 0.013±0.010 g were stocked in 24 tanks. The second experiment lasted 35 days, and shrimp at an initial weight of 0.037±0.002 g were stocked in six tanks. Weekly biometric measurements were performed to adjust the amount of feed. Suspended solids were higher at a density of 6000 PL m−3 and mosquito...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artificial substrates; Biofloc technology; Litopenaeus vannamei; Stocking density; Yield.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100104
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Immunization of hybrid surubim (Pseudoplatystoma corruscans x P. fasciatum) against Motile Aeromonas hydrophila septicemia BABT
Silva,Bruno Correa da; Jatobá,Adolfo; Vieira,Felipe do Nascimento; Mouriño,José Luiz Pedreira; Bolívar,Norha; Seiffert,Walter Quadros; Martins,Mauricio Laterça.
The aim of this study was to evaluate the immune response of hybrid surubim vaccinated by intraperitoneal injection and by immersion against Aeromonas hydrophila. Vaccine was prepared with formalin-inactivated A. hydrophila. Forty eight fishes (47±9g) were distributed in 12 tanks (4 fish / tank) in the following treatments (4 tanks / treatment): unvaccinated fish, vaccinated intraperitoneally and vaccinated by immersion. After 21 days, intraperitoneally vaccinated fishes showed the highest values of total protein and immunoglobulins, agglutination titer and serum antimicrobial activity. Lysozyme concentration in the serum was higher in the fishes vaccinated by immersion than unvaccinated fishes. Intraperitoneal vaccination induced the highest immune...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudoplatystoma; Aeromonas hydrophila; Vaccine; Immunology.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100010
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In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture PAB
Vieira,Felipe do Nascimento; Jatobá,Adolfo; Mouriño,José Luiz Pedreira; Vieira,Eduardo Alano; Soares,Mariana; Silva,Bruno Corrêa da; Seiffert,Walter Quadros; Martins,Maurício Laterça; Vinatea,Luis Alejandro.
The objective of this work was to isolate strains of lactic acid bacteria with probiotic potential from the digestive tract of marine shrimp (Litopenaeus vannamei), and to carry out in vitro selection based on multiple characters. The ideotype (ideal proposed strain) was defined by the highest averages for the traits maximum growth velocity, final count of viable cells, and inhibition halo against nine freshwater and marine pathogens, and by the lowest averages for the traits duplication time and resistance of strains to NaCl (1.5 and 3%), pH (6, 8, and 9), and biliary salts (5%). Mahalanobis distance (D²) was estimated among the evaluated strains, and the best ones were those with the shortest distances to the ideotype. Ten bacterial strains were isolated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Acid lactic bacteria; Bioprospection; Pathogen inhibition.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800030
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Lactic acid bacteria used as preservative in fresh feed for marine shrimp maturation PAB
Vieira,Genyess Ribeiro Arthur da Silva; Soares,Mariana; Ramírez,Norha Constanza Bolívar; Schleder,Delano Dias; Silva,Bruno Corrêa da; Mouriño,José Luiz Pedreira; Andreatta,Edemar Roberto; Vieira,Felipe do Nascimento.
Abstract The objective of this work was to evaluate the effect of Lactobacillus plantarum on the preservation of fresh mussels and its effect on the attractiveness, consumption, and midgut bacterial microbiota of Pacific white shrimp broodstock. The experiment evaluated mussels stored with L. plantarum at 4ºC. The controls were: mussels stored at -18ºC without L. plantarum; mussels stored at -18ºC with L. plantarum; and mussels stored at 4ºC without L. plantarum. Microbiological analyses on mussels were performed on days 1, 7, 15, 30, 45, and 60 after processing. Additionally, mussels preserved with L. plantarum and stored at 4ºC, and mussels stored at -18ºC without L. plantarum were evaluated after 15 days for attractiveness, consumption, and midgut...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Litopenaeus vannamei; Vibrio; Probiotic.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016001101799
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Métodos para determinação da digestibilidade aparente de dietas para camarão marinho suplementadas com probiótico PAB
Buglione-Neto,Celso; Mouriño,José Luiz; Vieira,Felipe do Nascimento; Silva,Bruno Corrêa da; Jatobá,Adolfo; Seiffert,Walter; Fracalossi,Débora Machado; Andreatta,Edemar.
O objetivo deste trabalho foi determinar o método de coleta de fezes mais adequado para camarões marinhos (Litopenaeus vannamei) na determinação do coeficiente de digestibilidade aparente da proteína bruta e da matéria seca de dietas comerciais, e avaliar o efeito da suplementação de Lactobacillus plantarum na dieta sobre o coeficiente de digestibilidade aparente da proteína, da energia e da matéria seca. Foram testados três sistemas de coletas de fezes: decantação, sifonamento e dissecação dos animais. A coleta por decantação foi o sistema mais adequado para a determinação in vivo dos coeficientes de digestibilidade aparente da proteína bruta e da matéria seca das dietas, por apresentar baixo grau de lixiviação das fezes e por ser de fácil execução. Nos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Litopenaeus vannamei; Digestibilidade; Nutrição.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800033
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Probiotic supplementation in diet and vaccination of hybrid surubim (Pseudoplatystoma reticulatum♀ x P. corruscans♂) Ciência Rural
Pereira,Gabriella do Vale; Jesus,Gabriel Fernandes Alves; Vieira,Felipe do Nascimento; Pereira,Scheila Anelise; Ushizima,Thiago Tetsuo; Mouriño,José Luiz Pedreira; Martins,Maurício Laterça.
ABSTRACT: A supplementary diet with the probiotic bacteria Weissella cibaria on the efficacy of surubim hybrid immunization against a specific hemorrhagic septicemia caused by Aeromonas hydrophila was evaluated on the following treatments: fish fed a supplemented probiotic diet, vaccinated fish and vaccinated fish fed a supplemented probiotic diet, and untreated fish (control). Fish from the probiotic treatments were fed a diet containing W. cibaria for 41 days. On the 15th day of the experiment, fish from vaccine treatments were intraperitoneally vaccinated, with posterior oral booster for four days. One week after the oral booster, three fish from each experimental unit were sampled. The probiotic supplementation increased the number of thrombocytes and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Vaccine; Lactic acid bacteria; Aeromonas hydrophila.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200348
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Replacement of fish meal by protein soybean concentrate in practical diets for Pacific white shrimp R. Bras. Zootec.
Soares,Mariana; Fracalossi,Débora Machado; Freitas,Luiz Eduardo Lima de; Rodrigues,Marysol Santos; Redig,Joselle Cursino; Mouriño,José Luiz Pedreira; Seiffert,Walter Quadros; Vieira,Felipe do Nascimento.
ABSTRACTThe objective of this work was to evaluate the performance of Litopenaeus vannameifed different levels (0, 25, 50, 75, and 100%) of soybean protein concentrate (63.07% crude protein, CP) to replace fish meal-by product (61.24% CP). The study was conducted in clear water in fifteen 800 L tanks equipped with aeration systems, constant heating (29 ºC), and daily water exchange (30%). Each tank was stocked with 37.5 shrimp/m3 (3.03±0.14 g). Feed was supplied four times a day, at 6% of the initial biomass, adjusted daily. After 42 days, the weight gain of shrimp fed diets with 0 and 25% protein replacement was higher than that observed in shrimp fed 100% replacement, and there were no differences among those fed the other diets. Feed efficiency and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Growth; Litopenaeus vannamei; Nutrition; Protein replacement.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000343
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Replacement of fishmeal for soy protein concentrate in diets for juvenile Litopenaeus vannamei in biofloc-based rearing system R. Bras. Zootec.
Jatobá,Adolfo; Vieira,Felipe do Nascimento; Silva,Bruno Corrêa da; Soares,Mariana; Mouriño,José Luiz Pedreira; Seiffert,Walter Quadros.
ABSTRACT This study aimed to assess the final body weight, weekly weight gain, yield, apparent feed efficiency, protein efficiency ratio, and feed intake of the Pacific white shrimp (Litopenaeus vannamei) fed four diets containing different levels of soy protein concentrate (SPC) as a replacement for fishmeal, reared in a super-intensive biofloc system. Diets consisted of replacing 209 g kg-1 fishmeal at 0, 33, 66, and 100% SPC. Shrimp were raised in a biofloc system using twelve experimental units stocked with 250 shrimp m-3 under constant aeration (O2>5mg L-1) and temperature (29±0.5 °C). No significant differences among treatments were observed based on water quality parameters. Shrimp fed diets with 0 and 33% substitution exhibited the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquaculture; Intensive system; Nutrition; Protein sources.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000900705
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Substituição da farinha de peixe em dietas para camarões marinhos cultivados em sistema bioflocos PAB
Scopel,Bruno Ricardo; Schveitzer,Rodrigo; Seiffert,Walter Quadros; Pierri,Vinícius; Arantes,Rafael da Fonseca; Vieira,Felipe do Nascimento; Vinatea,Luis Alejandro.
O objetivo deste trabalho foi avaliar a substituição de dois níveis de inclusão de farinha de peixe por outros ingredientes, nas dietas de Litopenaeus vannamei cultivados em sistema bioflocos. Foram avaliadas dietas sem uso de farinha de peixe (dieta A, 100% de substituição), com inclusão de 12,5% de farinha de peixe (dieta B, 40% de substituição) e direta controle com inclusão de 21% (dieta C, 0% de substituição). Nas dietas A e B, o farelo de soja e as farinhas de carne e vísceras foram os principais substitutos proteicos. Foram analisados os índices de desempenho dos camarões e os parâmetros físicos e químicos da água de cultivo. Os camarões alimentados com a dieta B apresentaram maior peso final (11,63±1,38 g), em comparação aos camarões alimentados...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Carcinicultura; Sistema heterotrófico.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000800020
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Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant PAB
Vieira,Felipe do Nascimento; Buglione Neto,Celso Carlos; Mouriño,José Luiz Pedreira; Jatobá,Adolfo; Ramirez,Cristina; Martins,Maurício Laterça; Barracco,Margherita Anna Antonia Maria; Vinatea,Luis Alejandro.
The objective of this work was to assess the time-related action of probiotic Lactobacillus plantarum in the bacterial microbiota of the digestive tract of Litopenaeus vannamei, and the relation of total haemocyte count and serum phenol oxidase activity of shrimp challenged with Vibrio harveyi. Shrimps were fed with a probiotic-supplemented diet, for eight days, then shifted to a commercial diet. Shrimps fed only with the commercial diet served as control. Evaluations were made on the 8th day of experiment and repeated two, four, six and eight days later. Total lactic bacteria in the digestive tract was higher until the 4th day of evaluation in the probiotic-supplemented group. Vibrio spp. counts were higher in the control at days zero and two. Until the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Vibrio harveyi; Total haemocyte count; Lactic bacteria; Phenol oxidase; Probiotic.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000600013
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Use of probiotic-supplemented diet on a Pacific white shrimp farm R. Bras. Zootec.
Vieira,Felipe do Nascimento; Jatobá,Adolfo; Mouriño,José Luiz Pedreira; Buglione Neto,Celso Carlos; Silva,Jairo Sousa da; Seiffert,Walter Quadros; Soares,Mariana; Vinatea,Luis Alejandro.
ABSTRACT The objective of this study was to evaluate the use of a diet supplemented with Lactobacillus plantarum on performance, immunological parameters, and bacterial microbiota of the digestive tract of white leg shrimp (Litopenaeus vannamei). We used six earthen ponds (1.2 ha) stocked with 14 shrimp m−2 (3 g mean weight). Three ponds received a diet supplemented with L. plantarum and three were maintained as control. The survival and feed efficiency of shrimp fed the supplemented diet were greater than that observed in shrimp fed the control diet, with survival values of 83.02±6.12% and 74.65±9.07% and feed efficiencies of 117.97±4.45% and 104.46±7.30%, respectively. However, we observed no differences in weight gain. The intestines of shrimp fed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immune response; Lactobacillus; Microbiology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000500203
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Utilização de bactérias ácido-lácticas isoladas do trato intestinal de tilápia-do-nilo como probiótico PAB
Jatobá,Adolfo; Vieira,Felipe do Nascimento; Buglione Neto,Celso; Silva,Bruno Corrêa; Mouriño,José Luís Pedreira; Jerônimo,Gabriela Tomas; Dotta,Geovana; Martins,Maurício Laterça.
O objetivo deste trabalho foi isolar bactérias ácido-lácticas do intestino de tilápias-do-nilo, e avaliar seu potencial probiótico. Foram isoladas cepas de bactérias ácido-lácticas, e foi avaliada a inibição aos patógenos in vitro. As cepas com os melhores resultados foram identificadas e utilizadas no experimento de colonização do trato intestinal de tilápias-do-nilo, via suplementação na dieta, em delineamento inteiramente ao acaso, com três tratamentos e quatro repetições. Foram avaliados: o total de bactérias, as bactérias ácido-lácticas, Vibrio ssp. e Pseudomonas ssp. A cepa com melhor resultado foi utilizada na infecção experimental, em delineamento inteiramente ao acaso, em esquema fatorial 2x3: dieta suplementada com a cepa e dieta-controle; e os...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus durans; Lactobacillus plantarum; Oreochromis niloticus; Hematologia; Infecção experimental.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000900015
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