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| Saldanha,Roze L.; Garcia,José E.; Dekker,Robert F. H.; Vilas-Boas,Laurival A.; Barbosa,Aneli M.. |
| Nine isolates of Botryosphaeria spp. were evaluated for their growth and the production of cell wall-lytic enzymes (laccase, pectinase and beta-1,3-glucanase) when grown on basal medium in the absence and presence of the laccase inducer, veratryl alcohol (VA). The genetic relationship among the nine isolates collected from different host plants was determined by RAPD analyses. ITS sequence analysis showed eight closely related isolates classified as Botryosphaeria rhodina, and one isolate classified as Botryosphaeria ribis. RAPD analysis resolved the isolates into three main clusters based upon levels of laccase and beta-1,3-glucanase activity. There appears to be no correlation between pectinase production and genetic diversity among the nine isolates.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Botryosphaeria isolates ITS and RAPD Laccase Pectinase beta-1; 3-Glucanase. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200013 |
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| Bogas,Andréa C.; Watanabe,Maria Angelica E.; Barbosa,Aneli; Vilas-Boas,Laurival A.; Bonatto,Ana C.; Dekker,Robert; Souza,Emanuel M.; Fungaro,Maria Helena P.. |
| A beta-glucosidase-like enzyme-encoding gene (bglH) of an endophytic Bacillus pumilus strain (CL16) was cloned using a shotgun genomic library constructed in Escherichia coli. The nucleotide sequence of the entire cloned fragment (2484 bp) was determined and characterized. An incomplete open reading frame (ORF) of 534 bp (ORF1) designated bglP and a complete ORF of 1419 bp (ORF2) designated bglH, located in the fragment, are organized in an operon. The protein deduced from 1419 bp (ORF2) had 472 amino acid residues without a characteristic signal peptide sequence, suggesting that the enzyme is localized in the cytoplasm. The amino acid sequence deduced from bglH gene had high similarity with beta-glucosidases from the glycosyl hydrolase family 1.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus pumilus; BglH; Glucosidase; Glycosyl hydrolase 1; PTS. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100018 |
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