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Registros recuperados: 5
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Assessment of viability of sperm cells of Litopenaeus vannamei on cryopreservation BABT
Uberti,Marcela Fornari; Vieira,Felipe do Nascimento; Salência,Helena Ragibo; Vieira,Genyess da Silva; Vinatea,Luis Alejandro.
Aiming at assessing the cryopreservation potential of Litopenaeus vannamei sperm cells, 74 spermatophores were manually extracted from the sexually mature individuals. After the toxicity test to define the cryoprotectant concentration, suspensions of spermatic cells were cryopreserved in the groups in freezing solutions comprising different cryoprotectants such as dimethyl sulfoxide (DMSO) and ethylene glycol (EG) at 10% concentration. Each treatment was divided in subgroups for storage in liquid nitrogen during 0, 30, 60 and 90 days, in triplicate. After thawing at 25ºC for 40 seconds, cell viability in the suspensions was analyzed under the microscope in eosin-nigrosin stain and flow cytometry. There were no significant differences between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryogenics; Semen; Marine shrimp; Cell viability.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300010
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In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture PAB
Vieira,Felipe do Nascimento; Jatobá,Adolfo; Mouriño,José Luiz Pedreira; Vieira,Eduardo Alano; Soares,Mariana; Silva,Bruno Corrêa da; Seiffert,Walter Quadros; Martins,Maurício Laterça; Vinatea,Luis Alejandro.
The objective of this work was to isolate strains of lactic acid bacteria with probiotic potential from the digestive tract of marine shrimp (Litopenaeus vannamei), and to carry out in vitro selection based on multiple characters. The ideotype (ideal proposed strain) was defined by the highest averages for the traits maximum growth velocity, final count of viable cells, and inhibition halo against nine freshwater and marine pathogens, and by the lowest averages for the traits duplication time and resistance of strains to NaCl (1.5 and 3%), pH (6, 8, and 9), and biliary salts (5%). Mahalanobis distance (D²) was estimated among the evaluated strains, and the best ones were those with the shortest distances to the ideotype. Ten bacterial strains were isolated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Acid lactic bacteria; Bioprospection; Pathogen inhibition.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800030
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Substituição da farinha de peixe em dietas para camarões marinhos cultivados em sistema bioflocos PAB
Scopel,Bruno Ricardo; Schveitzer,Rodrigo; Seiffert,Walter Quadros; Pierri,Vinícius; Arantes,Rafael da Fonseca; Vieira,Felipe do Nascimento; Vinatea,Luis Alejandro.
O objetivo deste trabalho foi avaliar a substituição de dois níveis de inclusão de farinha de peixe por outros ingredientes, nas dietas de Litopenaeus vannamei cultivados em sistema bioflocos. Foram avaliadas dietas sem uso de farinha de peixe (dieta A, 100% de substituição), com inclusão de 12,5% de farinha de peixe (dieta B, 40% de substituição) e direta controle com inclusão de 21% (dieta C, 0% de substituição). Nas dietas A e B, o farelo de soja e as farinhas de carne e vísceras foram os principais substitutos proteicos. Foram analisados os índices de desempenho dos camarões e os parâmetros físicos e químicos da água de cultivo. Os camarões alimentados com a dieta B apresentaram maior peso final (11,63±1,38 g), em comparação aos camarões alimentados...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Carcinicultura; Sistema heterotrófico.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000800020
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Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant PAB
Vieira,Felipe do Nascimento; Buglione Neto,Celso Carlos; Mouriño,José Luiz Pedreira; Jatobá,Adolfo; Ramirez,Cristina; Martins,Maurício Laterça; Barracco,Margherita Anna Antonia Maria; Vinatea,Luis Alejandro.
The objective of this work was to assess the time-related action of probiotic Lactobacillus plantarum in the bacterial microbiota of the digestive tract of Litopenaeus vannamei, and the relation of total haemocyte count and serum phenol oxidase activity of shrimp challenged with Vibrio harveyi. Shrimps were fed with a probiotic-supplemented diet, for eight days, then shifted to a commercial diet. Shrimps fed only with the commercial diet served as control. Evaluations were made on the 8th day of experiment and repeated two, four, six and eight days later. Total lactic bacteria in the digestive tract was higher until the 4th day of evaluation in the probiotic-supplemented group. Vibrio spp. counts were higher in the control at days zero and two. Until the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Vibrio harveyi; Total haemocyte count; Lactic bacteria; Phenol oxidase; Probiotic.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000600013
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Use of probiotic-supplemented diet on a Pacific white shrimp farm R. Bras. Zootec.
Vieira,Felipe do Nascimento; Jatobá,Adolfo; Mouriño,José Luiz Pedreira; Buglione Neto,Celso Carlos; Silva,Jairo Sousa da; Seiffert,Walter Quadros; Soares,Mariana; Vinatea,Luis Alejandro.
ABSTRACT The objective of this study was to evaluate the use of a diet supplemented with Lactobacillus plantarum on performance, immunological parameters, and bacterial microbiota of the digestive tract of white leg shrimp (Litopenaeus vannamei). We used six earthen ponds (1.2 ha) stocked with 14 shrimp m−2 (3 g mean weight). Three ponds received a diet supplemented with L. plantarum and three were maintained as control. The survival and feed efficiency of shrimp fed the supplemented diet were greater than that observed in shrimp fed the control diet, with survival values of 83.02±6.12% and 74.65±9.07% and feed efficiencies of 117.97±4.45% and 104.46±7.30%, respectively. However, we observed no differences in weight gain. The intestines of shrimp fed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immune response; Lactobacillus; Microbiology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000500203
Registros recuperados: 5
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