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Antimicrobial activity of bergenin from Endopleura uchi (Huber) Cuatrec Acta Amazonica
Silva,Saulo L. da; Oliveira,Viviane Guedes de; Yano,Tomomasa; Nunomura,Rita de Cássia Saraiva.
Endopleura uchi (Huber) Cuatrec. is an Amazon species traditionally used as treatment for inflammations and female disorders. Bergenin was isolated from ethyl acetate fraction of bark of E. uchi by using column chromatography over sephadex LH-20 and then silica gel 60 flash. Its structure was identified on the basis of its NMR spectra. The antimicrobial activity of bergenin and fractions of methanol extract of E. uchi were evaluated against ATCC microorganisms (Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Candida albicans, C. guilliermondii, Aspergillus flavus, A. nidulans). Clinically isolated strains of all of these microorganisms, along with C. tropicalis, A. niger, Shigella sonnei,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial; Antifungal; Candida; Humiriaceae; Uxi-amarelo.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672009000100019
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Colibacillosis in lambs is associated to type I heat-stable enterotoxin in a farm in São Paulo State, Brazil Ciência Rural
Aragão,Annelize Zambon Barbosa; Teocchi,Marcelo Ananias; Fregolente,Maria Clara Duarte; Gatti,Maria Silvia Viccari; Pires,Alexandre Vaz; Yano,Tomomasa.
Twenty seven (48.2%) culture supernatants of 56 Escherichia coli isolated from diarrheic lamb feces (7 to 10 days old) in São Paulo State, Brazil, presented positive results to suckling mice assay (fluid accumulation) but none caused cytopathic effects on Vero and CHO cells, indicating that these strains did not produced LT or VT toxins. PCR assays showed that these 27 E. coli strains harbored estA, that codifies for STa, but not for stx1, stx2 or cnf genes. The positive STa strains were checked for genes that codify for F41, F17 and K99 fimbriae, wich are considered colonization factors in ETEC. Only F17 was detect in two samples (7.4%). Twelve of 27 STa positive carried hlyA gene and presented hemolytic activity in blood Agar. Presence of rotavirus was...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Lambs; STa; Colibacillosis; Escherichia coli.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000500015
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Curli fimbria: an Escherichia coli adhesin associated with human cystitis BJM
Cordeiro,Melina Aparecida; Werle,Catierine Hirsch; Milanez,Guilherme Paier; Yano,Tomomasa.
Abstract Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Curli fimbria; Adhesion; Escherichia coli; UPEC.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200414
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Cytotoxic evaluation of essential oil from Casearia sylvestris Sw on human cancer cells and erythrocytes Acta Amazonica
Silva,Saulo Luís da; Chaar,Jamal da Silva; Figueiredo,Patrícia de Maria Silva; Yano,Tomomasa.
A Casearia sylvestris (Flacourtiaceae) é uma planta popularmente conhecida como "guaçatonga" e é usada por povos indígenas da América do sul (Brasil, Peru e Bolivia) no tratamento de muitas doenças, incluindo câncer. Estudos citotóxicos mostraram que esta planta apresenta um possível e interessante potencial antitumoral devido à presença de moléculas chamadas casearinas. Além disso, a composição do óleo essencial mostrou uma alta concentração de sesquiterpenos de alto potencial citotóxico. Neste trabalho, nós verificamos que o óleo essencial da C. sylvestris apresentou uma boa citotoxicidade seletiva contra as linhagens de células tumorais HeLa, A-549 and HT-29 (CD50 63,3, 60,7 e 90,6 µg.ml-1, respectivamente) quando comparada às células não-tumorais Vero...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citotoxicidade; Casearia sylvestris; Eritrócitos; Óleo essencial; MTT; Sesquiterpenos.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000100012
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Cytotoxic evaluation of essential oil from Zanthoxylum rhoifolium Lam. leaves Acta Amazonica
Silva,Saulo Luis da; Figueiredo,Patrícia Maria; Yano,Tomomasa.
Zanthoxylum rhoifolium Lam is a plant popularly used as antimicrobial, for malaria and inflammatory treatment. The essential oil of Z. rhoifolium was extracted and its cytotoxic effects against HeLa (human cervical carcinoma), A-549 (human lung carcinoma), HT-29 (human colon adenocarcinoma), Vero (monkey kidney) cell lines and mice macrophages were evaluated. Some of the terpenes of its essential oil (ß-caryophyllene, alpha-humulene, alpha -pinene, myrcene and linalool) were also tested to verify their possible influence in the oil cytotoxic activity. The results obtained permitted to confirm that the essential oil is cytotoxic against tumoral cells (CD50 = 82.3, 90.7 and 113.6 µg/ml for A-549, HeLa e HT-29 cell lines, respectively), while it did not show...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zanthoxylum; Essential oil; Cytotoxicity; SS-caryophyllene; Alpha-humulene.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672007000200015
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Cytotoxic factor secreted by Escherichia coli associated with sepsis facilitates transcytosis through human umbilical vein endothelial cell monolayers BJID
Tibo,Luiz Henrique Soares; Bertol,Jéssica Wildgrube; Bernedo-Navarro,Robert Alvin; Yano,Tomomasa.
Abstract Culture supernatant of sepsis-associated Escherichia coli (SEPEC) isolated from patients with sepsis caused loss of intercellular junctions and elongation of human umbilical vein endothelial cells (HUVEC). The cytotoxic factor was purified from culture supernatant of SEPEC 15 (serogroup O153) by liquid chromatography process. PAGE (polyacrylamide gel electrophoresis) showed that the purified SEPEC cytotoxic factor had a molecular mass of ∼150 kDa and consisted of at least two subunits. At the concentration of 1 CD50 (40 μg/mL) did facilitate transcytosis through the HUVEC cells monolayer of SEPEC 15 as much as E. coli K12 within 30 min without affecting cell viability. These results suggest that this cytotoxic factor, named as SPF (SEPEC's...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cytotoxin (SPF); Sepsis; Transcytosis; Transendothelial electrical resistance.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300014
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Hemagglutinating properties of Salmonella enterica serovar Enteritidis isolated from different sources BJM
Mikcha,Jane M.G.; Ferreira,Antonio J. Piantino; Ferreira,Claudete S. Astolfi; Yano,Tomomasa.
Twenty-five strains of Salmonella enterica serovar Enteritidis isolated from different sources were examined for hemagglutinating activity. Bacteria cultured in different media induced hemagglutination of human erythrocytes, but no reaction was observed with erythrocytes from other animal species. The hemagglutinating expression activity was better for cultures on CFA agar at 37ºC than other conditions examined. The hemagglutination was inhibited by D-mannose, D-mannitol, melibiose, D-raffinose, L-rhamnose and sucrose. The absence of cell-surface appendages in electron microscope examinations suggested a nonfimbrial hemagglutinin. The data suggest that Salmonella Enteritidis produces nonfimbrial mannose-sensitive hemagglutinin, specific for human...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella Enteritidis; Hemagglutinating activity; Mannose-sensitive hemagglutinin; Nonfimbrial hemagglutinin.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100008
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Plesiomonas shigelloides exports a lethal cytotoxic-enterotoxin (LCE) by membrane vesicles BJID
Ludovico,Marilucia Santos; Martins,Luciano Moura; Bianco,Juares Ednaldo Romero; Andrade,Célia Guadalupe Tardelli de Jesus; Falcon,Rosabel; Joazeiro,Paulo Pinto; Gatti,Maria Silvia Viccari; Yano,Tomomasa.
ABSTRACT Plesiomonas shigelloides isolated from water in Brazil was previously described as a hemorrhagic heat-labile cytotoxic-enterotoxin producer. We purified this toxin from culture supernatants using ion metallic affinity chromatography (IMAC) followed by molecular exclusion chromatography. The pure toxin presented molecular mass of 50 kDa and isoelectric point (pI) around 6.9 by 2D electrophoresis. When injected intravenously, the purified cytotoxic-enterotoxin induced also severe spasms followed by sudden death of mice. Hence, we entitled it as lethal cytotoxic-enterotoxin (LCE). The presence of membrane vesicles (MVs) on cell surfaces of P. shigelloides was observed by scan electron microscopy (SEM). From these MVs the LCE toxin was extracted and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lethal cytotoxic enterotoxin (LCE); Membrane vesicles (MVs); Plesiomonas shigelloides; Toxin transportation; Virulence factors.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600546
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Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil BJM
Salvadori,Márcia Regina; Valadares,Geórgio Freesz; Leite,Domingos da Silva; Blanco,Jesús; Yano,Tomomasa.
Two hundred and five Escherichia coli strains isolated from calves with diarrhea from mid-western Brazil were screened for the presence of virulence factors associated with bovine colibacillosis. One hundred and two (49.8%) of the E. coli strains produced toxins: Shiga toxins 1 (9.7%) and 2 (6.3%), a-hemolysin (9.7%), enterohemolysin(6.8%), Cytotoxic Necrotizing Factors type 1 (0.5%), and type 2 (4.4%), enterotoxins LT-II (8.3%) and STa (3.9%). No strain produced enterotoxin LT-I. Fimbrial adhesins F5 and F17 were produced by 7.3% and 4.8% of the strains, respectivly, and none expressed F41. Seven strains (3.4%) possessed the gene eae and belonged to serotypes O26:H-; O111:H- and O118:H16. These results suggest that calves in Brazil may be an important...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calves; Diarrhea; E. coli; Virulence factors.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300009
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Virulence-associated characteristics of Enterococcus faecalis strains isolated from clinical sources BJM
Furumura,Márcia T.; Figueiredo,Patricia M.S.; Carbonell,Gleize V.; Darini,Ana Lucia da Costa; Yano,Tomomasa.
Thirty-two clinical isolates of Enterococcus faecalis were screened for virulence factors. Twenty-four (75%) isolates produced hemolysin on Mueller-Hinton blood agar plates with sheep erythrocytes. However, the cell free heat-stable hemolysin was detected in all isolates (100%) of E. faecalis when grown in BHI-GA (BHI medium supplemented with 1% glucose and 0.03% L-arginine), but not in BHI broth alone. Twenty-four isolates (75%) produced caseinase and 23 (71.9%) lipase, but none of the isolates produced gelatinase. Fifteen (46.9%) culture filtrates caused rounding and membrane alterations with blebbing formation followed by death in HeLa and HEp-2 cells, but not in Vero cells. Thirteen isolates (40.6%) agglutinated rabbit erythrocytes, but did not produce...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus faecalis; Virulence factors; Hemolysin; Proteases; Lipase; Cytotoxin; Biofilm.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300007
Registros recuperados: 10
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