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Optimization of Bacillus subtilis cell growth effecting jiean-peptide production in fed batch fermentation using central composite design Electron. J. Biotechnol.
Zhong,Juan; Zhang,Xiaoyong; Ren,Yanli; Yang,Jie; Tan,Hong; Zhou,Jinyan.
Background Optimization of nutrient feeding was developed to improve the growth of Bacillus subtilis in fed batch fermentation to increase the production of jiean-peptide (JAA). A central composite design (CCD) was used to obtain a model describing the relationship between glucose, total nitrogen, and the maximum cell dry weight in the culture broth with fed batch fermentation in a 5 L fermentor. Results The results were analyzed using response surface methodology (RSM), and the optimized values of glucose and total nitrogen concentration were 30.70 g/L and 1.68 g/L in the culture, respectively. The highest cell dry weight was improved to 77.50 g/L in fed batch fermentation, which is 280% higher than the batch fermentation concentration (20.37 g/L). This...
Tipo: Journal article Palavras-chave: Bacillus subtilis ZK8; Iturin A; Response surface methodology.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300005
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Response surface methodology used for statistical optimization of jiean-peptide production by Bacillus subtilis cells adsorbed on wood chips Electron. J. Biotechnol.
Zhang,Xiaoyong; Zhou,Jinyan; Fu,Wen; Li,Zhidong; Zhong,Juan; Yang,Jie; Xiao,Liang; Tan,Hong.
Response surface methodology (RSM) was used for statistical optimization of jiean-peptide (JAA) production by Bacillus subtilis ZK8 cells adsorbed on wood chips to form a novel fermentation system. The Plackett-Burman design was used in the first step to evaluate the effects of eight factors, including six fermentation medium components and two cell adsorption conditions. Among the variables screened, soybean meal hydrolysate (SMH) and MgSO4·7H2O in the fermentation medium had significanteffects on JAA production. In the second step, the concentrations of SMH and MgSO4·7H2O were further optimized using central composite designs and response surface analysis. The optimized concentration of SMH and MgSO4·7H2O was 24% (v/v) and 0.38% (w/v), respectively,...
Tipo: Journal article Palavras-chave: Bacillus subtilis ZK8; Cell adsorption; Jiean- peptide; Response surface methodology; Wood chips.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400006
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