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Registros recuperados: 28
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A microbial community analysis of the octocoral Eunicea fusca Electron. J. Biotechnol.
Duque-Alarcón,Angela; Santiago-Vázquez,Lory Z; Kerr,Russell G.
While there is a significant and growing body of knowledge describing the microbial communities of marine invertebrates such as sponges, there are very few such studies focused on octocorals. The octocoral Eunicea fusca is common on reefs in various regions of the Caribbean and has been the subject of natural product investigations. As part of an effort to describe the microbial community associated with octocorals, a culture-independent analysis of the bacterial community of E. fusca was conducted. Specifically, a 16S rDNA clone library analysis was performed to provide baseline data. A total of 40 bacteria members from 11 groups were found. In general, Proteobacteria were the dominant group with a total of 24 species and α-Proteobacteria...
Tipo: Journal article Palavras-chave: 16S rDNA; Bacteria; Culture-independent analysis; Eunicea fusca; Microbial community; Octocoral.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500015
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A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms Electron. J. Biotechnol.
Revelo Romo,Dolly Margot; Grosso,María Victoria; Moreno Solano,Nubia Carmenza; Montoya Castaño,Dolly.
A molecular approach was used for selecting polyhydroxyalcanoate (PHA)-accumulating potential Gram-negative bacteria from different genera by colony polymerase chain reaction (PCR). Three degenerate primers were designed for amplifying a fragment from PHA synthase gene (phaC) (Class I), phaC1 and phaC2 (Class II) genes for detecting PHA-producing bacteria. Thirty-four out of 55 bacterial strains from the old collection selected using Sudan black B staining were phaC+. PCR was used for directly selecting 35 new collection bacterial strains; these strains were phaC+ and their ability to produce PHA was confirmed by Sudan black B staining. Four specific primers were designed on genes of Class II PHA biosynthesis operon. These primers were used for evaluating...
Tipo: Journal article Palavras-chave: 16S rDNA; Colony PCR; Degenerate primers; PHA synthase; Polyhydroxyalcanoate; Seminested PCR.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300002
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A note on the systematic position of the Bathynellacea (Crustacea, Malacostraca) using molecular evidence Naturalis
Camacho, Ana Isabel; Rey, Isabel; Dorda, Beatriz A.; Machordom, Annie; Valdecasas, Antonio G..
Molecular data for the mt 16S rDNA gene fragment of a bathynellacean is here presented for the first time and used to analyze the relationship of the group within the crustacean class Malacostraca (Arthropoda, Bathynellacea). Two contrasting views have classified the bathynelids as being cither within the order Syncarida or in a separate super-order Podophallocarida belonging to the infra-class Eonomostraca, a disagreement based mainly on debates over external and internal morphology. The preliminary analyses offered here in question the placement of this Bathynellacea within the Syncarida, and suggest the need for a further study of relationships among the malacostracan groups.
Tipo: Article / Letter to the editor Palavras-chave: Systematics; Crustacea; Malacostraca; Bathynellacea; 16S rDNA.
Ano: 2002 URL: http://www.repository.naturalis.nl/record/534365
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Análise das relações taxonômicas e sistemáticas entre espécies de triatomíneos (Hemiptera, Reduviidae) de colônias mantidas pelo Serviço Especial de Saúde de Araraquara, inferida de seqüências do 16S rDNA mitocondrial Rev. Bras. entomol.
Ceretti Junior,Walter; Vendrami,Daniel Pagotto; Gil,João Molina; Barata,José Maria Soares; Marrelli,Mauro Toledo.
Foram analisadas seqüências de nucleotídeos do gene 16S do rDNA mitocondrial em 14 populações de triatomíneos mantidos em colônias no insetário SESA de Araraquara- SP, comparando-as com seqüências do mesmo gene disponíveis no GenBank. Os fragmentos variaram de 311 a 317 pb com baixa variação intra-específica entre as distâncias genéticas (0% a 0,6%), exceto para os relacionamentos entre espécimes de Triatoma sordida (1%) e espécimes de T. brasiliensis (1,3%) atribuídos a populações geográficas diferentes. A parafilia de Rhodniini e do gênero Panstrongylus foi evidenciada pelas analises, confirmando resultados anteriores entre estes e os estreitos relacionamentos de R. prolixus com R. robustus e de T. infestans e T. platensis. O relacionamento entre T....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triatominae; Vetores da doença de Chagas; Sistemática; 16S rDNA.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262008000300021
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Atividade enzimática e perfil da comunidade bacteriana em solo submetido à solarização e biofumigação PAB
Passos,Samuel Ribeiro; Reis Junior,Fábio Bueno dos; Rumjanek,Norma Gouvêa; Mendes,Ieda de Carvalho; Baptista,Miriam Josefina; Xavier,Gustavo Ribeiro.
O objetivo deste trabalho foi avaliar o efeito da solarização e da biofumigação sobre a comunidade microbiana do solo, por meio da atividade da enzima beta-glicosidase e do perfil do 16S rDNA, determinado com PCR-DGGE. A solarização do solo, com cobertura de plástico, foi feita por períodos de dois, quatro e seis meses, e a biofumigação foi realizada pela incorporação de 2 e 5% (v/v) de cama-de-frango ao solo. Logo após a retirada da cobertura de plástico e aos 30 dias após a remoção, a atividade da beta-glicosidase foi menor em relação ao tratamento não solarizado. Aos 60 dias, não foram mais observadas diferenças entre os tratamentos. A adição de cama-de-frango a 5% estimulou a atividade da beta-glicosidase. O perfil da estrutura da comunidade bacteriana...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beta-glicosidase; Cama-de-frango; Diversidade microbiana; Ecologia microbiana; Manejo do solo; 16S rDNA.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700012
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Bacterial diversity characterization in petroleum samples from Brazilian reservoirs BJM
Oliveira,Valéria Maia de; Sette,Lara Durães; Simioni,Karen Christina Marques; Santos Neto,Eugênio Vaz dos.
This study aimed at evaluating potential differences among the bacterial communities from formation water and oil samples originated from biodegraded and non-biodegraded Brazilian petroleum reservoirs by using a PCR-DGGE based approach. Environmental DNA was isolated and used in PCR reactions with bacterial primers, followed by separation of 16S rDNA fragments in the DGGE. PCR products were also cloned and sequenced, aiming at the taxonomic affiliation of the community members. The fingerprints obtained allowed the direct comparison among the bacterial communities from oil samples presenting distinct degrees of biodegradation, as well as between the communities of formation water and oil sample from the non-biodegraded reservoir. Very similar DGGE band...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Community DNA; Cultivation-independent analysis; Petroleum; 16S rDNA.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300007
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Bacterial diversity in the sediments of a temperate artificial lake, Rapel reservoir RChHN
DORADOR,CRISTINA; CASTILLO,GABRIELA; WITZEL,K. P.; VILA,IRMA.
Rapel reservoir is an eutrophic system in Chile that has undergone a series of anthropogenic impacts in recent decades. To provide a better understanding of the processes occurring in the reservoir, we examined bacterial composition from surface sediments using traditional microbiology techniques and molecular biology tools. Our results showed significant temporal variation in the physical and chemical composition of the water column, but no depth-related differences during the study period. To detect temporal changes in bacterial composition, cultivable heterotrophic bacteria, heterotrophic iron oxidizing bacteria, and sulfate-reducing bacteria were extracted from the surface sediments and their concentration measured. Microbial diversity in sediments was...
Tipo: Journal article Palavras-chave: Sediments; Sulfate-reducing bacteria; 16S rDNA.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2007000200007
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Barriers in the pelagic: population structuring of Calanus helgolandicus and C. euxinus in European waters Naturalis
Yebra, L.; Bonnet, D.; Harris, R.P.; Lindeque, P.K.; Peijnenburg, K.T.C.A..
Molecular studies of marine plankton have shown that ecological and/or environmental barriers play an important role in separating populations. Calanoid copepods are central in marine ecosystems, and dramatic biogeographical shifts in copepod assemblages associated with recent climate warming have been reported. We examined spatial population structuring in European waters of the Atlantic Ocean and Mediterranean Sea of Calanus helgolandicus and its sister species, C. euxi nus, from the Black Sea based on genetic and morphometric characters. The aims were to identify barriers to dispersal, relate these to hydrographic characteristics and infer historical patterns of distribution and demography. We analysed a 408 bp fragment of the mitochondrial 16S gene...
Tipo: Article / Letter to the editor Palavras-chave: Calanus helgolandicus; Calanus euxinus; 16S rDNA; Mediterranean Sea; North Atlantic Ocean; Black Sea; 42.74.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/470574
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Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India BJM
Parvathi,Ammini; Krishna,Kiran; Jose,Jiya; Joseph,Neetha; Nair,Santha.
Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus pumilus; FAME; 16S rDNA; CesB; AP-PCR.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200012
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Comparative molecular analysis of Herbaspirillum strains by RAPD, RFLP, and 16S rDNA sequencing Genet. Mol. Biol.
Soares-Ramos,Juliana R.L.; Ramos,Humberto J.O.; Cruz,Leonardo M.; Chubatsu,Leda S.; Pedrosa,Fábio O.; Rigo,Liu U.; Souza,Emanuel M..
Herbaspirillum spp. are endophytic diazotrophic bacteria associated with important agricultural crops. In this work, we analyzed six strains of H. seropedicae (Z78, M2, ZA69, ZA95, Z152, and Z67) and one strain of H. rubrisubalbicans (M4) by restriction fragment length polymorphism (RFLP) using HindIII or DraI restriction endonucleases, random amplified polymorphic DNA (RAPD), and partial sequencing of 16S rDNA. The results of these analyses ascribed the strains studied to three distinct groups: group I, consisting of M2 and M4; group II, of ZA69; and group III, of ZA95, Z78, Z67, and Z152. RAPD fingerprinting showed a higher variability than the other methods, and each strain had a unique electrophoretic pattern with five of the six primers used....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Herbaspirillum; RAPD; RFLP; Phylogeny; 16S rDNA.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400019
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Detection of Wolbachia (Alphaproteobacteria: rickettsiales) in three species of terrestrial isopods (crustacea: isopoda: oniscidea) in Brazil BJM
Zimmermann,Bianca Laís; Almerão,Maurício Pereira; Bouchon,Didier; Araujo,Paula Beatriz.
Terrestrial isopods are widely infected with Wolbachia. However, little is known about the presence of bacteria in the Neotropical species. The objective of this study was to test the hypothesis of presence of Wolbachia infection in the native species of terrestrial isopods, Atlantoscia floridana and Circoniscus bezzii, and in the introduced species Burmoniscus meeusei.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wolbachia; Terrestrial isopods; 16S rDNA; Brazil.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200036
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Eficiência simbiótica de estirpes de Bradyrhizobium isoladas de solo do Cerrado em caupi PAB
Zilli,Jerri Édson; Valicheski,Romano Roberto; Rumjanek,Norma Gouvêa; Simões-Araújo,Jean Luis; Freire Filho,Francisco Rodrigues; Neves,Maria Cristina Prata.
O objetivo deste trabalho foi avaliar as relações filogenéticas de estirpes de Bradyrhizobium e a contribuição destas estirpes para a fixação biológica de nitrogênio em caupi, em solos do Cerrado. Na avaliação da relação filogenética, o gene 16S rDNA de cada uma das estirpes foi amplificado e seqüenciado, e para a análise da eficiência simbiótica, determinou-se: N total, matéria seca das plantas, massa de nódulos e redução de acetileno, em casa de vegetação, e ocupação nodular, em experimento de campo. A maioria das estirpes estudadas pertence a B. elkanii e, pelo menos dez das estirpes, independentemente da espécie, apresentaram bom desempenho quanto à fixação biológica de N2. As estirpes BR3262, BR3280 (caracterizadas como B. elkanii) e BR3267, BR3287 e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fixação biológica de nitrogênio; 16S rDNA; Bradyrhizobium; Bradyrhizobium elkanii.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000500013
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Endophytic bacterial diversity in banana 'Prata Anã' (Musa spp.) roots Genet. Mol. Biol.
Souza,Suzane A.; Xavier,Adelica A.; Costa,Márcia R.; Cardoso,Acleide M.S.; Pereira,Marlon C.T.; Nietsche,Silvia.
The genetic diversity of endophytic bacteria in banana 'Prata Anã' roots was characterized. Two hundred and one endophytic bacteria were isolated, 151 of which were classified as Gram-positive and 50 as Gram-negative. No hypersensitivity response was observed in any of the isolates. The rep-PCR technique generated different molecular profiles for each primer set (REP, ERIC and BOX). Fifty readable loci were obtained and all of the fragments were polymorphic. Amplified ribosomal DNA restriction analysis (ARDRA) of the isolates based on cleavage with four restriction enzymes yielded 45 polymorphic bands and no monomorphic bands. PCR amplified the nifH gene in 24 isolates. 16S rDNA sequencing of the 201 bacterial isolates yielded 102 high-quality sequences....
Tipo: Info:eu-repo/semantics/article Palavras-chave: ARDRA; Bacillus sp.; NifH gene; Rep-PCR; 16S rDNA.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200016
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Increased genetic diversity in Greek populations of the genus Ligidium (Crustacea: Isopoda: Oniscidea) revealed by RFLP analysis of mtDNA segments Naturalis
Klossa-Kilia, E.; Kilias, G.; Sfenthourakis, S..
We investigated mtDNA genetic differentiation and the phylogenetic relationships of 11 populations of the oniscidean genus Ligidium. We studied nine populations from Greece, assigned to three nominal species (L. euboicum, L. germanicum and L. beieri), and two from central Europe (L. germanicum and L. hypnorum), applying RFLP analysis of two mtDNA segments (12S rDNA and 16S rDNA) amplified by PCR. The analysis revealed seven monomorphic and four polymorphic populations, and 17 different haplotypes, each one present in one population only. Several restriction enzymes produced population specific patterns, enabling discrimination among the different populations. The estimated net nucleotide divergence between populations ranges from 1.06 to 16.52%, while the...
Tipo: Article / Letter to the editor Palavras-chave: Oniscidea; Greece; MtDNA; RLFPs; Genetic isolation; Ligidium; Isopods; 16S rDNA; 12S rDNA; 42.74.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/209650
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Isolation and characterization of endophytic bacteria isolated from the leaves of the common bean (Phaseolus vulgaris) BJM
Costa,Leonardo Emanuel de Oliveira; Queiroz,Marisa Vieira de; Borges,Arnaldo Chaer; Moraes,Celia Alencar de; Araújo,Elza Fernandes de.
The common bean is one of the most important legumes in the human diet, but little is known about the endophytic bacteria associated with the leaves of this plant. The objective of this study was to characterize the culturable endophytic bacteria of common bean (Phaseolus vulgaris. leaves from three different cultivars (Vermelhinho, Talismã, and Ouro Negro) grown under the same field conditions. The density of endophytic populations varied from 4.5 x 10² to 2.8 x 10³ CFU g-1 of fresh weight. Of the 158 total isolates, 36.7% belonged to the Proteobacteria, 32.9% to Firmicutes, 29.7% to Actinobacteria, and 0.6% to Bacteroidetes. The three P. vulgaris cultivars showed class distribution differences among Actinobacteria, Alphaproteobacteria and Bacilli. Based...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Common bean; Phaseolus vulgaris; 16S rDNA; Diversity indices.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400041
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Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats Genet. Mol. Biol.
Falcon,Tiago; Foletto,Kelly Carraro; Siebert,Marina; Pinto,Denise Entrudo; Andrades,Michael; Bertoluci,Marcello Casaccia.
Abstract The effects of non-nutritive sweeteners (NNS) on the gut microbiota are an area of increasing research interest due to their potential influence on weight gain, insulin resistance, and inflammation. Studies have shown that mice and rats fed saccharin develop weight gain and metabolic alterations, possibly related to changes in gut microbiota. Here, we hypothesized that chronic exposure to a commercial NNS would change the gut microbiota composition in Wistar rats when compared to sucrose exposure. To test this hypothesis, Wistar rats were fed either NNS- or sucrose-supplemented yogurt for 17 weeks alongside standard chow (ad libitum). The gut microbiome was assessed by 16S rDNA deep sequencing. Assembly and quantification were conducted using the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 16S rDNA; Deep sequencing; Saccharin; Cyclamate; Yogurt.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100805
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Morphological and molecular characterization of Isostichopus sp. in the Colombian Caribbean sea JBAG
Vergara,W; Agudelo,V; Castro,L; R. Rodríguez,A; Eeckhaut,I.
Isostichopus sp. (Aspidochirotida: Stichopodidae) are sea cucumbers widely distributed in the Caribbean Sea. Amongst them, Isostichopus badionotus is one of the most harvested species. It shows a wide range of morphotypes widespread in the Caribbean region including different habitats (muddy, sandy and rocky bottoms, and sea grass beds). In Colombia, three morphotypes can be distinguished; two of them live in sea grass beds while the third one is found on rocky substrates. The present study describes the morphological characteristics of these morphotypes and analyzes their genetic structures through 16S rDNA and COI data. Our phylogenetic analyses show that the morphotype living on rocky substrates is morphologically and genetically distinct from the two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cucumbers; COI; 16S rDNA; Isostichopus badionotus.
Ano: 2018 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332018000200004
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New host record of five Flavobacterium species associated with tropical fresh water farmed fishes from North India BJM
Verma,Dev Kumar; Rathore,Gaurav.
Abstract Yellow pigmented, filamentous, Gram-negative bacteria belonging to genus Flavobacterium are commonly associated with infections in stressed fish. In this study, inter-species diversity of Flavobacterium was studied in apparently healthy freshwater farmed fishes. For this, ninety one yellow pigmented bacteria were isolated from skin and gill samples (n = 38) of three farmed fish species i.e. Labeo rohita, Catla catla and Cyprinus carpio. Among them, only twelve bacterial isolates (13.18%) were identified as Flavobacterium spp. on the basis of morphological, biochemical tests, partial 16S rDNA gene sequencing and phylogenetic analysis. On the basis of 16S rDNA gene sequencing, all the 12 isolates were 97.6-100% similar to six different formally...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flavobacterium; Fish; Species diversity; 16S rDNA; Phylogenetic analysis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000400969
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Partial characterization of nif genes from the bacterium Azospirillum amazonense BJMBR
Potrich,D.P.; Passaglia,L.M.P.; Schrank,I.S..
Azospirillum amazonense revealed genomic organization patterns of the nitrogen fixation genes similar to those of the distantly related species A. brasilense. Our work suggests that A. brasilense nifHDK, nifENX, fixABC operons and nifA and glnB genes may be structurally homologous to the counterpart genes of A. amazonense. This is the first analysis revealing homology between A. brasilense nif genes and the A. amazonense genome. Sequence analysis of PCR amplification products revealed similarities between the amino acid sequences of the highly conserved nifD and glnB genes of A. amazonense and related genes of A. brasilense and other bacteria. However, the A. amazonense non-coding regions (the upstream activator sequence region and the region between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum amazonense; Nif genes; GlnB; 16S rDNA.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900002
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Phylogenetic relationships between Bacillus species and related genera inferred from 16s rDNA sequences BJM
Wei Wang,Mi Sun.
Neigh-borjoining, maximum-parsimony, minimum-evolution, maximum-likelihood and Bayesian trees constructed based on 16S rDNA sequences of 181 type strains of Bacillus species and related taxa manifested nine phylogenetic groups. The phylogenetic analysis showed that Bacillus was not a monophyletic group. B. subtilis was in Group 1. Group 4, 6 and 8 respectively consisted of thermophiles, halophilic or halotolerant bacilli and alkaliphilic bacilli. Group 2, 4 and 8 consisting of Bacillus species and related genera demonstrated that the current taxonomic system did not agree well with the 16S rDNA evolutionary trees. The position of Caryophanaceae and Planococcaceae in Group 2 suggested that they might be transferred into Bacillaceae, and the heterogeneity of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus phylogeny; Bayesian inference; Evolutionary trees; 16S rDNA.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300013
Registros recuperados: 28
Primeira ... 12 ... Última
 

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