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Cytotoxic potential of waters of the streams Mandacaru, Maringá, Miosótis and Nazareth in the urban area of Maringá, Paraná State, Brazil - doi: 10.4025/actascibiolsci.v34i3.8275 Biological Sciences
Düsman, Elisângela; Universidade Estadual de Maringá; Gonçalves, Larissa Aparecida; Universidade Estadual de Maringá; Reusing, Ana Flávia; Universidade Estadual de Maringá; Martin, Paula Garcia; Universidade Estadual de Maringá; Mariucci, Rosinete Gonçalves; Universidade Estadual de Maringá; Vicentini, Veronica Elisa Pimenta; Universidade Estadual de Maringá.
Streams Mandacaru, Maringá, Miosótis and Nazareth in the urban area of Maringá, Estado do Paraná, Brazil are affected by the local population’s polluting practices. Meristemic cells of Allium cepa L. roots were used as test system to evaluate the cytotoxic effects of the streams’ water. Onion roots were prepared by Feulgen reaction and stained with Schiff's reagent. Statistical analysis of the mitotic index was undertaken with the chi-square test. Results did not show significant cytotoxic effect of water samples. Results may dependent on climate variations, concentration of harmful substances, and on the time and samples sites evaluated. A deeper awareness of the population should be underscored for the proper use, preservation and maintenance of soil and...
Palavras-chave: 2.02.00.00-5; 2.02.06.00-3 cytotoxicity; Stream water; Allium cepa L. Genética.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8275
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Evaluation of the genotoxicity of zinc oxide-eugenol cement to Allium cepa L. - doi: 10.4025/actascibiolsci.v35i4.17925 Biological Sciences
Rezende, Elisângela de Fátima; Centro Universitário de Lavras; Mendes-Costa, Maria Cristina; Centro Universitário de Lavras; Fonseca, Johnson Campideli; Centro Universitário de Lavras; Ribeiro, Alex Oliveira; Centro Universitário de Lavras.
Evaluation of the Genotoxicity of Zinc Oxide-Eugenol Cement in Allium cepa L. Dental materials can induce local and systemic effects. The Allium cepa assay was used to evaluate the genotoxicity and/or cytotoxicity of zinc oxide and eugenol (ZOE) at different proportions. The ZOE solution was tested at the concentration of 1 drop of eugenol (in each drop of liquid, the approximate concentration of eugenol is 85%) and 1 portion of zinc oxide cement (treatment I), and twice the concentration of eugenol (treatment II). Treated roots appeared to be yellowish-brown, fewer in number, thicker and less turgid compared with the control, suggesting a cytotoxic activity of ZOE. A significant difference was found in the root size between the control and treatment II....
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.00.00.00-6; 2.02.00.00-5; 2.02.06.00-3 cytotoxicity; Micronucleus (MN); Dental materials; Mutagenesis Genética.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17925
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Vegetal test-system investigation on cytotoxicity of water from urban streams located in the northeastern region of Maringá, Paraná State, Brazil - doi: 10.4025/actascibiolsci.v33i1.4924 Biological Sciences
Düsman, Elisângela; Universidade Estadual de Maringá; Faria, Juliana Sala de; Universidade Estadual de Maringá; Toledo, Fabiana; Universidade Estadual de Maringá; Mazeti, Camila Montoro; Universidade Estadual de Maringá; Gonçalves, Marcus Eduardo Kamide; Universidade Estadual de Maringá; Vicentini, Veronica Elisa Pimenta; Universidade Estadual de Maringá.
The increase in consumption of water, the destruction of riparian forests and the pollution caused by humans, have severely degraded several water resources. Numerous stream crisscross the city of Maringá, Paraná state, Brazil and most of their sources are scattered through the urban region. Current analysis assesses the cytotoxic potential of water from the Corregozinho, Isalto, Morangueira and Ozório streams, located within the high-populated northeastern region of Maringá, inside the urban perimeter. Root meristematic cells of Allium cepa were used as test-system. The roots of onion were prepared by Feulgen’s reaction and stained with Schiff's reagent. Results showed that there were no statistically significant changes evaluated by the chi-square test...
Palavras-chave: 2.02.00.00-5; 2.02.06.00-3 environmental mutagenesis; Water contamination; Allium cepa Mutagênese environmental mutagenesis; Water contamination; Allium cepa.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4924
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