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A simple negative selection method to identify adenovirus recombinants using colony PCR Electron. J. Biotechnol.
Zhao,Yongliang; Dong,Zongming; Li,Tengfei; Huang,Gang.
Background: The AdEasy system is a fast-track system for generating recombinant adenoviruses using the efficient homologous recombination machinery between shuttle and adenovirus backbone plasmids in Escherichia coli BJ5183 cells. The key step is homologous recombination in BJ5183 cells, which is driven by RecA activity. However, culture time is stringently limited to reduce the damage to recombinant plasmids by RecA activity. Therefore, rapid identification of recombinant adenoviruses within the limited time-period is critical. Results: We developed a simple negative selection method to identify recombinant adenoviruses using colony PCR, which improves the efficiency of adenovirus recombination screening and packaging. Conclusions: The negative selection...
Tipo: Journal article Palavras-chave: Adenovirus; AdEasy; Colony PCR; Homologous recombination; RecA.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100008
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Bioaccumulation of animal adenoviruses in the pink shrimp BJM
Luz,Roger B.; Staggemeier,Rodrigo; Fabres,Rafael B.; Soliman,Mayra C.; Souza,Fernanda G.; Gonçalves,Raoni; Fausto,Ivone V.; Rigotto,Caroline; Heinzelmann,Larissa S.; Henzel,Andréia; Fleck,Juliane D.; Spilki,Fernando R..
Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100) Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR) in order to detect and quantify viral...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenovirus; Farfantepenaeus paulensis; Pink-shrimp; Water quality; Northern Coast of Rio Grande do Sul.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300715
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Construction of a recombinant adenovirus co-expressing truncated human prostate-specific membrane antigen and mouse 4-1BBL genes and its effect on dendritic cells BJMBR
Weng,Xiaodong; Kuang,Youlin; Liu,Xiuheng; Chen,Zhiyuan; Zhu,Hengcheng; Chen,Hui; Jiang,Botao; Shen,Hao.
Our aim was to construct a recombinant adenovirus co-expressing truncated human prostate-specific membrane antigen (tPSMA) and mouse 4-1BBL genes and to determine its effect on dendritic cells (DCs) generated from bone marrow suspensions harvested from C57BL/6 mice for which the effect of 4-1BBL on DCs is not clear, especially during DCs processing tumor-associated antigen. Replication deficient adenovirus AdMaxTM Expression System was used to construct recombinant adenovirus Ad-tPSMA-internal ribosome entry site-mouse 4-1BBL (Ad-tPSMA-IRES-m4-1BBL) and Ad-enhanced green fluorescent protein. Day 7 proliferating DC aggregates generated from C57BL/6 mice were collected as immature DCs and further mature DCs were obtained by lipopolysaccharide activated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prostate cancer; PSMA; Co-stimulatory molecule; 4-1BBL; Adenovirus; Dendritic cells.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300002
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Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection BJID
Paulini,Inarei; Siqueira-Silva,Joselma; Thomaz,Luciana; Rocha,Leticia; Harsi,Charlotte; Bellei,Nancy; Granato,Celso.
Abstract Human adenoviruses comprise an important group of etiologic agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenovirus; Monoclonal antibodies; Immunochromatographic test.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500500
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Evaluation of skimmed milk flocculation method for virus recovery from tomatoes BJM
Melgaço,Fabiana Gil; Corrêa,Adriana Abreu; Ganime,Ana Carolina; Brandão,Marcelo Luiz Lima; Medeiros,Valéria de Mello; Rosas,Carla de Oliveira; Lopes,Silvia Maria dos Reis; Miagostovich,Marize Pereira.
Abstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenovirus; Flocculation; Murine norovirus-1; Norovirus; Tomato.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500034
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Identification of Adenovirus 7h Heterogeneity in the E3 Region Biol. Res.
BRUZZONE,MARISOL; BARRO,MARIO; SPENCER,EUGENIO.
Adenovirus genotype 7h was previously reported to be originated from a recombination event between adenovirus genotypes 7p and 3p. Based on those findings, further characterization of other adenovirus 7h strains become important to determine whether all adenovirus 7h strains arose from a single recombinational event. To explore such a possibility, 160 clinical isolates were studied after developing a PCR assay using a primer set designed to amplify the region corresponding to E3-7,7 Kd of adenovirus ADV 7p and E3-9 Kd of adenovirus 3p. The assay was able to differentiate most of the subgenus B strains from adeno 7h with the genotype 3d. The study of several adenovirus 7h clinical isolates revealed the existence of three variants of adeno7h. One of the...
Tipo: Journal article Palavras-chave: Adenovirus.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200010
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Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study BJID
Akçay,Emine; Çarhan,Ahmet; Hondur,Gözde; Tufan,Zeliha Koçak; Duru,Necati; Kılıç,Selçuk; Ensari,Ezgi Naz; Uğurlu,Nagihan; Çağıl,Nurullah.
Abstract Background: Viral conjunctivitis are the most frequent infections in ophthalmology clinics. The diagnosis is usually relying on clinical findings and medical history. However, topical antibiotics are often used unnecessarily addition to symptomatic treatment because of unsure agents. We aimed to detect the Adenovirus, Coxsackievirus and Enterovirus from conjunctiva and pharyngeal samples of patients. Methods: The conjunctiva and pharyngeal samples of the patients with conjunctivitis were taken by Virocult transport media and kept at -80 ºC up to study day. Adenovirus spp, Enterovirus 70 and Enterovirus 71, Coxsackie A24 and Coxsackie A16 were detected by real-time PCR. Samples from healthy health care workers of ophthalmology clinic were used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenovirus; Conjunctivitis; Viral; PCR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400391
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Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor BJMBR
Xu,H.H.; Liu,S.H.; Guo,Q.F.; Liu,Q.H.; Li,X.Y..
Bone morphogenetic protein 2 (BMP2) and basic fibroblast growth factor (bFGF) have been shown to exhibit a synergistic effect to promote bone repair and healing. In this study, we constructed a novel adenovirus with high coexpression of BMP2 and bFGF and evaluated its effect on osteogenic differentiation of goat bone marrow progenitor cells (BMPCs). Recombinant adenovirus Ad-BMP2-bFGF was constructed by using the T2A sequence. BMPCs were isolated from goats by density gradient centrifugation and adherent cell culture, and were then infected with Ad-BMP2-bFGF or Ad-BMP2. Expression of BMP2 and bFGF was detected by ELISA, and alkaline phosphatase (ALP) activity was detected by an ALP assay kit. In addition, von Kossa staining and immunocytochemical staining...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BMP2; BFGF; Adenovirus; T2A sequence; BMPCs; Osteogenic differentiation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900809
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p19Arf sensitizes B16 melanoma cells to interferon-β delivered via mesenchymal stem cells in vitro BJMBR
Da-Costa,R.C.; Vieira,I.L.; Hunger,A.; Tamura,R.E.; Strauss,B.E..
The immune stimulatory and anti-neoplastic functions of type I interferon have long been applied for the treatment of melanoma. However, the systemic application of high levels of this recombinant protein is often met with toxicity. An approach that provides localized, yet transient, production of type I interferon may overcome this limitation. We propose that the use of mesenchymal stem cells (MSCs) as delivery vehicles for the production of interferon-β (IFNβ) may be beneficial when applied together with our cancer gene therapy approach. In our previous studies, we have shown that adenovirus-mediated gene therapy with IFNβ was especially effective in combination with p19Arf gene transfer, resulting in immunogenic cell death. Here we showed that MSCs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene therapy; Immunotherapy; Stem cell therapy; Adenovirus; P53; Interferon-β.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300601
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Pathology of naturally occurring porcine adenovirus type 4 infection in Japan OAK
Agungpriyono, Dewi Ratih; Nakagawa, Michio; Morozumi, Tetsuo; Yoshihara, Shinobu; Furuoka, Hidefumi; Matsui, Takane; アグリプリヨノ, デヴィ ラテイ; 中川, 迪夫; 両角, 徹雄; 吉原, 忍; 古岡, 秀文; 松井, 高峯.
Palavras-chave: Adenovirus; Encephalitis; Inclusion body; Nephritis; Swine.
Ano: 1997 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1326
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Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy International Journal of Morphology
Catroxo,M. H. B; Martins,A. M. C. R. P. F; Petrella,S; Curi,N. A; Melo,N.A.
The pigeon feces are vehicle of diseases both for humans and other animal species. In these birds, the most important viral diseases of the digestive tract are transmitted by the paramyxovirus, adenovirus and coronavirus. Avian paramyxoviruses have been isolated from a variety of species of free living and domestic birds worldwide, with several symptoms and clinical signs and economic losses. Paramyxoviruses belong to the Paramyxoviridae family and Avulovirus genus that includes nine serotypes (APMV 1 to 9). Avian adenoviruses belong to the Adenoviridae family and Aviadenovirus genus. In pigeons, cause classical adenovirosis and necrotizing hepatitis. The respiratory and enteric tracts are common targets of coronavirus. They belong to the Coronaviridae...
Tipo: Journal article Palavras-chave: Paramyxovirus; Adenovirus; Coronavirus; Pigeons; Transmission electron microscopy.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200055
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Simultaneous viral infection and childhood bronchiolitis obliterans BJID
Hirschheimer,Mário; Silva,Paulo S. L.; Giudici,Roseli; Carrilho,Milene; Mauad,Thais; Ishida,Maria.
Lower respiratory tract infections by respiratory syncytial virus (RSV) are frequent in childhood. Association of RSV with bronchiolitis obliterans has rarely been established. We report a 13-month-old child with bronchiolitis obliterans following co-infection by RSV and adenovirus, and suggest that complicated evolution of an acute bronchiolitis case can indicate an association of pathogens.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute bronchiolitis; Adenovirus; Bronchiolitis obliterans; Co-infection; Lower respiratory tract infections; Respiratory syncytial virus.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000300009
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Uso de tratamento enzimático para testar viabilidade de adenovírus porcino isolado de dejeto suíno. Repositório Alice
VIANCELLI, A.; GARCIA, L. A. T.; STEINMETZ, R. L. R.; KUNZ, A.; ESTEVES, P. A.; CELIA REGINA M. BARARDI.
2011
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Dejeto suíno; Adenovirus; DNAse; Virologia; Viabilidade viral.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/914130
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