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| Registros recuperados: 23 | |
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| Thimoteo,S.S.; Glogauer,A.; Faoro,H.; de Souza,E.M.; Huergo,L.F.; Moerschbacher,B.M.; Pedrosa,F.O.. |
| Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene named metachi18A. The metachi18A gene was subcloned and overexpressed in Escherichia coli BL21 and the MetaChi18A chitinase was purified by affinity chromatography as a 6xHis-tagged fusion protein. The MetaChi18A enzyme is a 92-kDa protein with a conserved active site domain of glycosyl hydrolases family 18. It hydrolyses colloidal chitin with an optimum pH of 5 and temperature of 50°C. Moreover, the enzyme... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chitinase; Metagenomic; Aeromonas; Kinetics. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100602 |
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| Prediger,Karoline de Campos; Pereira,Renata da Silva; Winckler Neto,Carlos Hugo Del Priore; Santos,Roberto Christ Vianna; Fadel-Picheth,Cyntia Maria Telles; Vizzotto,Bruno Stefanello. |
| Aeromonas spp. were identified in five (2,7%) of 182 diarrheal stool cultures, A. caviae was predominant, resistant mainly to ampicillin and cephalotin. This is the first study showing the presence of Aeromonas spp. in diarrheal stools of outpatients in the central region of Rio Grande do Sul State, Brazil. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas; Stools; Outpatients; Multi-resistance. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300017 |
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| Guerra,Ivani M.F.; Fadanelli,Raquel; Figueiró,Manuela; Schreiner,Fernando; Delamare,Ana Paula L.; Wollheim,Claudia; Costa,Sérgio Olavo P.; Echeverrigaray,Sergio. |
| Aeromonas were isolated from 27 (6.6%) of 408 patients admitted with acute gastroenteritis in two hospitals at Rio Grande do Sul, Brazil. Isolates were classified as A. hydrophila (51.8%), A. caviae (40.8%), and A. veronii biotype sobria (7.4%). The highest prevalence of Aeromonas associated infections occurred in lactants and children. Virulence genes (aerA -aerolysin/hemolysin, ahpA -serine-protease, satA - glycerophospholipid-cholesterol acyltransferase, lipA -lipase, and ahyB -elastase) and virulence factors (hemolytic, proteolitic, lipolitic activities, and biofilm formation) were identified in most A. hydrophila and A. veronii biotype sobria isolates, with lower frequencies on A. caviae. All Aeromonas isolates were resistant to ampicillin,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas; Diarrhea disease; Virulence factors; Antimicrobial resistance. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400011 |
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| CERESER, N. D.; ROSSI JÚNIOR, O. D.; MARTINELI, T. M.; SOUZA, V. de; RODRIGUES, L. B.; CARDOZO, M. V.. |
| Resumo: Com o objetivo de estabelecer, durante o processamento do queijo Minas Frescal e Colonial os possíveis pontos de contaminação e a forma de disseminação de bactérias do gênero Aeromonas, foram analisados, quanto à presença do micro-organismo, diferentes produtos e pontos do fluxograma de produção. Para o Queijo Minas Frescal, Aeromonas spp. foram isoladas no leite cru, leite pasteurizado, ambiente de produção e nas mãos dos manipuladores. A. caviae foi a espécie mais frequentemente identificada, sendo também isoladas A. sobria e A. schubertii. Durante o processamento do queijo Colonial, as espécies A. hydrophila, A. caviae, A. sobria, A. veronii e A. jandaei foram isoladas a partir da água, mãos dos manipuladores, utensílios, leite cru e após... |
| Tipo: Artigo de periódico |
Palavras-chave: Queijo Minas Frescal; Queijo Colonial; Produção artesanal; Cheese.; Bacteria; Microrganismo; Contaminação bacteriana; Higiene de alimento; Microbiologia de alimento; Consumidor; Análise de risco; Aeromonas; Microbiology; Food contamination; Risk analysis.. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/973437 |
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| Di Bari,Marisa; Hachich,Elayse M.; Melo,Adalgisa M.J.; Sato,Maria I. Z.. |
| Aeromonas species are autochtonous in the aquatic environment and some of them have been associated with health effects like wound infections, septicemia and diarrhoeal illness. In this study, the occurrence of Aeromonas spp. and microbial indicators in raw drinking water from wells, springs, fountains and mineral waters was evaluated. A total of 126 water samples was analyzed for Aeromonas spp. by the membrane filtration technique using ADA media and by P/A test. Typical colonies of Aeromonas spp. were submitted to biochemical tests for species differentiation. Toxin production was tested using Y-1 mouse adrenal cells. Coliforms and heterotrophic bacteria were enumerated by membrane filtration and pour plate techniques, respectively. P. aeruginosa, C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas; Raw drinking water; Microbial indicators; Heat labile toxin. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300025 |
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| SEBASTIÃO, F. A.; MAJOLO, C.; MARTINS, V. F. S.; BOIJINK, C. de L.; BRANDÃO, F. R.; PEREIRA, S. L. A.; FUJIMOTO, R. Y.; CHAGAS, E. C.. |
| Bacterial diseases are important factors that limit productivity in aquaculture. To reduce negative economic impacts, fish farmers use antimicrobials, often indiscriminately, and this action has led to bacterial resistance to drugs. The objectives of this study were to isolate and identify the main putative pathogenic bacterial species in tambaqui (Colossoma macropomum), establish the profile of resistance to antimicrobials by the methods of disc diffusion, and determine the minimum inhibitory concentration (MIC) values. Two hundred and ninety asymptomatic fish were collected between March and November 2015 from ten fish farms in the Amazonas state (Brazil). As doenças bacterianas são fatores importantes que limitam a produtividade na aquicultura. Para... |
| Tipo: Artigo de periódico |
Palavras-chave: Antibióticos; Fish farming; Piscicultura; Tambaqui; Aeromonas; Antibiotics. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150935 |
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| Bizani,Delmar; Brandelli,Adriano. |
| The presence of Aeromonas spp. in food has been demonstrated. They are often introduced from water, which is the natural habitat of many species and is thought to be the main source of contamination. The occurrence of Aeromonas spp. was investigated in 70 water samples of a bovine abattoir. Aeromonas spp. were present in 21.4% of water samples. A. hydrophila was isolated from 11.4% of supplying water samples and from 25.7% of the water drained from carcasses, whereas A. sobria was sole isolated from 5.7% of supplying water samples. Although greater number of positive samples were from water drained from carcasses, only A. hydrophila isolates were present. This suggests that the supplying water is the source of contamination. The antibiotic susceptibility... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas; Antibiotic activity; Aquatic environment; Hemolysis; Water. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400016 |
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| Sousa,José Américo de; Silva-Souza,Ângela Teresa. |
| A bacteriological study was conducted on fish and water from Congonhas River, Sertaneja (22º58 S; 50º58 W), Paraná State, Brazil. From 44% of the analysed fish, bacteria belonging to Pseudomonas, Acinetobacter, Aeromonas, Enterobacteriaceae, Micrococcus, Bacillus and Lactobacillus were isolated. The group most frequently isolated from fish was Aeromonas. In the water, the bacterial groups detected were Pseudomonas, Acinetobacter, Aeromonas, Enterobacteriaceae, Bacillus and Flavobacterium, from which Flavobacterium and Acinetobacter were the most abundant. The numbers of Colony Forming Units per millilitre of water varied from 3.1x10² to 1.0 x 10³. Although a clear pattern was not detected in the susceptibilities/resistances of the isolated strains to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial community; Fish; Congonhas River; Aeromonas; Pseudomonas; Resistance. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400007 |
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| Evangelista-Barreto,Norma Suely; Carvalho,Fatima Cristiane Teles de; Vieira,Regine Helena S. dos Fernandes; dos Reis,Cristhiane Moura Falavina; Macrae,Andrew; Rodrigues,Dália dos Prazeres. |
| Thirty water samples were collected, at two week intervals, from the estuary of the River Cocó. The aim was to characterize the presence, distribution and types of Aeromonas spp, in the estuary of the River Cocó, Ceara, Brazil (03º46'28.83''S e 38º26'36.52''S). Aeromonas were identified in 19 (63%) samples analyzed by plating and CFU counts. Presence/absence tests were positive for 11 (37%) of the samples resulting in the detection of Aeromonas in a total of 23 (77%) of samples. CFU counts varied from < 10 to 1.4 x 10(4) CFU mL-1 . From the isolated strains seven species of Aeromonas were identified: A. caviae (29/69), A. veronii bv. sobria (13/69), A. veronii bv. veronii (8/69), A. trota (6/69), A. media (5/69), A. sobria (4/69) and A. hydrophila and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas; Estuary; Antibiotics. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200027 |
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| Rosso,Fernando; Cedano,Jorge Andrés; Parra-Lara,Luis Gabriel; Sanz,Ana María; Toala,Alejandra; Velez,Jolian Fernando; Hormaza,María Paula; Moncada,Pablo Andrés; Correa,Adriana. |
| ABSTRACT Introduction: Aeromonas species are renowned enteric pathogens with virulence determinants linked to human diseases, such as gastroenteritis, skin, soft-tissue and muscle infections, and septicemia. A recent concern of resistance in this organism has emerged, especially the presence carbapenemases. Herein we describe a case series of emerging carbapenem-resistant Aeromonas species infection in our hospital in Cali, Colombia. Materials and methods: Cases from 2012 to 2018 are reported. Clinical data was abstracted from the clinical charts and laboratory information. Phenotypic detection of resistance was identified using the VITEK®2 system (BioMérieux) and broth microdilution MicroScan WalkAway plus System (Beckman Coulter). CARBA NP-test and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas; Aeromonas hydrophila; Carbapenems; CphA protein; Real-time polymerase chain. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500336 |
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| Bulhões,C.C.C.; Rossi Júnior,O.D.. |
| Foram analisadas 160 amostras de queijo-de-minas frescal artesanal, adquiridas no comércio varejista dos municípios de Poços de Caldas - MG e Jaboticabal - SP, a fim de verificar a ocorrência de bactérias do gênero Aeromonas no produto. Oitenta e duas (51,2%) encontravam-se contaminadas pelos microrganismos, com populações que variavam de 5,0×10³ a 4,0×10(5) UFC/grama. Foram identificadas as espécies Aeromonas hydrophila, Aeromonas caviae, Aeromonas schubertii, além de cepas consideradas atípicas. Os resultados evidenciam que bactérias do gênero Aeromonas podem ser veiculadas através do queijo tipo minas frescal artesanal e devem servir de alerta aos serviços de saúde pública. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas; Queijo-de-minas frescal; Artesanal. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000300017 |
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| Balsalobre,Livia Carminato; Dropa,Milena; de Oliveira,Danielle Escudeiro; Lincopan,Nilton; Mamizuka,Elsa Masae; Matté,Glavur Rogério; Matté,Maria Helena. |
| It is known that Aeromonas spp. possess different chromosomal β-lactamase genes. Presence and phenotypic expression of blaTEM, blaSHV, and blaCTX-M ESBL-encoding genes were investigated in environmental water isolates of Aeromonas hydrophila and Aeromonas jandaei. Presence of blaSHV and blaCTX-M genes was not observed, and blaTEM gene was verified in 91% of the isolates. Sequencing of 10 fragments showed the occurrence of blaTEM-116. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas; Environmental isolates. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300023 |
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| Registros recuperados: 23 | |
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