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Aneuploidy and its relationship with growth in different populations of the Pacific oyster (Crassostrea gigas, Thunberg) ArchiMer
Boudry, Pierre; Leitao, Alexandra; Mccombie, Helen; Thiriot-quievreux, Catherine.
Cytogenetic abnormalities arising both in mitosis and meiosis are known to be common in bivalves. Here we review result obtained from the observations of somatic aneuploidy in different populations of the commercially important Pacific oyster, Crassostrea gigas. The oysters studied were either (1) produced in IFREMER hatcheries between 1986 and 1999, (some of these were part of the EU funded programme GENEPHYS); or (2) collected from wild populations along the French Atlantic coast. Chromosome numbers were scored from 30 mitotic metaphases in gill tissue per individual studied. Aneuploid cells (2n - X) were observed in all the 13 populations studied. A highly significant negative correlation was observed between the level of aneuploidy and growth, i.e....
Tipo: Text Palavras-chave: Growth; Genetic; Aneuploidy; Crassostrea gigas; Pacific oysters.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3446.pdf
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Apport de la génétique pour une meilleure connaissance des populations naturelles et une amélioration des populations cultivées d'huîtres plates et creuses ArchiMer
Lapegue, Sylvie.
In the framework of the French diploma "Habilitation à Diriger des Recherches", Sylvie Lapègue's synthesis of research includes ten years (1998-2007) of work in bivalve genetics, and mainly cupped and flat oysters. This research has been developed in (1) flat oyster population genetics at the European level and cupped oyster populations and species genetics at a global scale, (2) the characterization of a cytogenetic particularity observed in oysters that corresponds to a high rate of aneuploidy in somatic cells, and (3) more recently in the use of molecular markers for the construction or improvement of genetic maps, allowing (4) the identification of areas of the genome related to the resistance to summer mortality in the Pacific oyster and the...
Tipo: Text Palavras-chave: Ostrea; Crassostrea; Oysters; QTLs; Genetic maps; Breeding; Aneuploidy; Population genetics; Ostrea; Crassostrea; Huître; QTLs; Cartes génétiques; Amélioration; Aneuploïdie; Génétique des populations.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/these-3478.pdf
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Autotetraploid Pacific oysters (Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability ArchiMer
Benabdelmouna, Abdellah; Ledu, Christophe.
We describe two methods of producing viable and fertile autotetraploid Pacific oyster (Crassostrea gigas Thunberg) based on the use of normal-sized oocytes produced by normal diploid females. Our methods showed that the oocyte size is not a limiting factor for the success of the induction to autotetraploidy. These methods offer means of direct introgression of genetic progress from elite diploid lines to tetraploids used as broodstock, avoiding a triploid step with the risk of transferring undesirable traits from highly fecund triploids. High variability in the level of cytogenetic stability was found among the different tetraploid oysters tested showing that induction method has an important impact on the long-term cytogenetic stability of the...
Tipo: Text Palavras-chave: Oyster; Tetraploid induction; Mosaicism; Aneuploidy; Cytogenetic stability.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00278/38924/37464.pdf
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Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas ArchiMer
Bouilly, Karine; Gagnaire, Beatrice; Bonnard, Marc; Thomas-guyon, Helene; Renault, Tristan; Miramand, Pierre; Lapegue, Sylvie.
Pacific oysters, Crassostrea gigas, are commonly reared in estuaries where they are exposed to anthropogenic pollution. Much research has been made on the toxicity of cadmium to aquatic organisms because the compound recurrently contaminates their environment. Our study examined the influence of cadmium on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in C. gigas at different stages of life. Adults and juveniles were exposed to two different concentrations of cadmium. The first concentration applied was equivalent to a peak value found in Marennes-Oleron bay (Charente-Maritime, France; 50 ng L-1) and the second was 10 times higher (500 ng L-1). Exposure to 50 ng L-1 cadmium caused a significant...
Tipo: Text Palavras-chave: Phenoloxidase; Flow cytometry; Hemocytes; Aneuploidy; Crassostrea gigas; Cadmium.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1687.pdf
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Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas ArchiMer
Bouilly, Karine; Leitao, Alexandra; Chaves, Raquel; Guedes Pinto, Henrique; Boudry, Pierre; Lapegue, Sylvie.
Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique. Pairs 1, 5, 9, and 10 are characterized by the loss of 1 chromosome. As restriction enzyme (RE) digestion chromosome banding allows a better identification of chromosome pairs, we used this technique to identify which chromosomes are affected when aneuploidy is increased by exposure to atrazine. The progeny of oysters contaminated by atrazine were...
Tipo: Text Palavras-chave: Crassostrea gigas; Pacific oyster; Restriction enzyme digestion chromosome banding; Atrazine; Aneuploidy; Crassostrea gigas; Huître creuse; Marquage chromosomique par digestion d'enzymes de restriction; Atrazine; Aneuploïdie.
Ano: 2005 URL: https://archimer.ifremer.fr/doc/2005/publication-654.pdf
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Evidence of differential chromosome loss in aneuploid karyotypes of the Pacific oyster, Crassostrea gigas ArchiMer
Leitao, Alexandra; Boudry, Pierre; Thiriot Quievreux, Catherine.
The G-banding technique was performed on aneuploid karyotypes from gill tissue of the Pacific oyster, Crassostrea gigas, to assess whether chromosome losses could be explained by differential chromosomal susceptibility and to clarify the negative correlation between aneuploidy and growth rate previously reported in different populations of this oyster. The study of 95 G-banded aneuploid karyotypes showed that only 4 of the 10 chromosome pairs (viz. 1, 5, 9, and 10) of C. gigas were affected by the loss of one homologous chromosome. Pairs 1, 9, and 10, which were lost in 56, 33, and 44% of cases, respectively, may be considered to be differentially affected. Hypotheses on this differential chromosomal susceptibility are discussed.
Tipo: Text Palavras-chave: Oyster; Karyotype; G banding; Growth; Aneuploidy; Huître; Caryotype; Bandes G; Croissance; Aneuploïdie.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-742.pdf
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Experimental evidence for a genetic basis to differences in aneuploidy in the Pacific oyster (Crassostrea gigas) ArchiMer
Leitao, Alexandra; Boudry, Pierre; Mccombie, Helen; Gerard, Andre; Thiriot-quievreux, Catherine.
Aneuploidy has been previously reported in wild and cultivated Pacific oysters, Crassostrea gigas, and has been shown to be negatively correlated with growth. This is especially important since high variability of growth rate is one of the major problems in the aquacultural production of this species. The existence of a genetic basis for the observed differences in aneuploidy was first investigated through the comparative study of six full-sib families, with mean individual weights ranging from 0.59 to 1.49 g. The slowest growing family was found to have the highest level of aneuploidy. Significant differences in aneuploidy were also found among families when individuals with the same growth rate were sampled. This supports the hypothesis of the existence...
Tipo: Text Palavras-chave: Oyster; Growth; Genetics; Full sib families; Crassostrea gigas; Aneuploidy; Huître; Croissance; Génétique; Famille de plein frères; Crassostrea gigas; Aneuploïdie.
Ano: 2001 URL: https://archimer.ifremer.fr/doc/2001/publication-2700.pdf
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Fluorescence in situ hybridization (FISH) or rDNA genes (5S and 18-5.8-28S) as a tool for chromosomal tagging and to assess the occurrence of somatic and sperm aneuploidy in Pacific oyster Crassostrea gigas ArchiMer
Benabdelmouna, Abdellah; Nadolna, Katarzyna; Ledu, Christophe.
Aneuploidy (or alteration of chromosome number) has been frequently described in diploid, triploid and tetraploid C. gigas. In diploid Pacific oysters, aneuploidy affects 1 to 3 chromosomes in up to 35% of the somatic cells and is positively correlated with inferior growth of the animal and thus could be associated with significant economic loss in this major aquaculture species. In polyploid Pacific oysters, aneuploidy was shown to be more heavily frequent and is linked to reversion to lower ploidy levels and is at the origin of aberrant gametes formation. In this context, identification of individual chromosomes involved in aneuploidy events both in somatic and germinal cells would be an important step towards the study of the exact occurrence of this...
Tipo: Text Palavras-chave: Chromosome; Aneuploidy; Genetic; Crassostrea gigas; Pacific oysters.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-3502.pdf
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Genetic transmission of Solanum demissum (2n=6x=72) chromosomes from a pentaploid hybrid of S. tuberosum (2n=4x=48) into the aneuploid BC1 progeny OAK
Ono, Seijiro; Sanetomo, Rena; Hosaka, Kazuyoshi.
Solanum demissum is a homozygous, hexaploid wild species (2n = 6x = 72, AADDD(d)D(d)) that has long been used as a source of late blight resistance by repeated backcrossing with S. tuberosum (2n = 4x = 48, AAA(t)A(t)). To understand how S. demissum germplasm is incorporated to S. tuberosum through backcrosses, a linkage analysis of 590 DNA markers from S. demissum-specific simplex alleles was performed in an aneuploid population of 87 BC1 plants. Five hundred eighty-one markers were mapped to 38 linkage groups, of which 37 were assigned to potato chromosomes using markers of known location. Three homoeologous chromosomes were distinguished for each of the 12 potato chromosomes. Approximately 50 % of the markers were transmitted to the BC1 progeny except...
Palavras-chave: Solanum demissum; Allohexaploid species; Genetic map; Aneuploidy; Preferential chromosome pairing; Potato.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4457
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Genetic variation among and within tritipyrum (Thinopyrum bessarabicum x Triticum durum) lines using PCR-based molecular markers Ciencia e Investigación Agraria
Siahsar,Barat Ali; Allahdoo,Maryam; Shahsevand Hassani,Hossein.
Primary hexaploid tritipyrum lines are amphiploids between Triticum durum and Thinopyrum bessarabicum, which can set seed in at least 250 mM NaCl. Genetic variation within and among 14 lines of primary tritipyrum were investigated by using 2 random and 6 semi random primers that produced clear and reproducible DNA amplicons. Average values for Nei's gene diversity (h) and Shannon indices (I) were 0.115 and 0.178, respectively. These results indicated low variation within primary tritipyrum lines, probably related to unbiased chromosome and partial aneuploidy. Total genetic diversity (Ht) and gene diversity within lines (Hs) were 0.2114 and 0.1156, respectively. The coefficient of genetic differentiation (Gst) was equal to 0.4534. AMOVA showed highly...
Tipo: Journal article Palavras-chave: Aneuploidy; Genetic variation; Random primers; Semi random primers; Tritipyrum lines.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100012
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Impact de facteurs environnementaux sur l'aneuploïdie chez l'huître creuse, Crassostrea gigas, dans le bassin de Marennes-Oléron ArchiMer
Bouilly, Karine.
The impact of environmental factors on aneuploidy level in the oyster Crassostrea gigas was studied with experiments in controlled conditions which exposed adult and juvenile oysters to different pollutants (atrazine, a herbicide, and cadmium, a heavy metal) frequently found in Marennes-Oléron Bay. A positive correlation between atrazine concentration and aneuploidy in C. gigas was observed. Moreover, the impact of atrazine on aneuploidy level persisted in time both within and between generations, demonstrating the influence of an environmental pollution on this phenomenon. On the other hand, the aneuploidy level of C. gigas was not influenced at the cadmium concentrations tested. Significant differences in aneuploidy levels were observed in oysters...
Tipo: Text Palavras-chave: Marennes Oléron Bay; Cadmium; Atrazine; Environmental factors; Aneuploidy; Crassostrea gigas; Bassin de Marennes Oléron; Cadmium; Atrazine; Facteurs environnementaux; Aneuploïdie; Crassostrea gigas.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/these-326.pdf
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Impact of atrazine on somatic aneuploidy in cupped oysters, Crassostrea gigas ArchiMer
Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Chaves, R.; Guedes Pinto, H; Boudry, Pierre; Lapegue, Sylvie.
In the Pacific oyster, Crassostrea gigas hypodiploid aneuploid cells with 2n=19, 18 or 17 chromosomes have regularly been reported. There is a negative correlation between somatic aneuploidy and growth rate in oysters (Leitão et al., 2001a) as well as evidence for a genetic basis (Leitão et al., 2001b). Furthermore, non-random chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique (Leitão et al., 2001c). This poster summarises different studies used to investigate the effects of the herbicide atrazine on the level of aneuploidy in this species
Tipo: Text Palavras-chave: Genetic; Aneuploidy; Toxicology; Impact; Atrazine; Crassostrea gigas; Pacific oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3489.pdf
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Impact of diuron on aneuploidy and hemocyte parameters in Pacific oyster, Crassostrea gigas ArchiMer
Bouilly, Karine; Bonnard, Marc; Gagnaire, Beatrice; Renault, Tristan; Lapegue, Sylvie.
Diuron is a substituted urea herbicide used for agricultural and nonagricultural weed control. Its widespread use and relatively slow breakdown led us to analyze its influence on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in Pacific oysters, Crassostrea gigas. Adult oysters were subjected to two diuron concentrations (300 ng L-1 and 3 mu g L-1) for 11 weeks. Significantly higher aneuploidy level was observed in diuron-treated oysters compared with the control. Furthermore, the observed impact on aneuploidy persisted to the next generation as offspring exhibited significantly higher aneuploidy levels when their parents had been exposed to diuron. Significant increases in hemocyte parameters (cell...
Tipo: Text Palavras-chave: Diuron; Herbicide impact; Cytometry; Hemocyte; Aneuploidy; Crassostrea gigas.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2178.pdf
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Impact of pollutants on aneuploidy in the Pacific oyster, Crassostrea gigas ArchiMer
Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Miramand, Pierre; Lapegue, Sylvie.
Aneuploidy is the alteration of the normal diploid chromosome number. In the Pacifie oyster, Crassastrea gigas, hypodiploid cells have regularly been reported as have a negative correlation between this phenomenon and growth and evidence for a genetic basis. We previously demonstrated a positive relationship between a pollutant, atrazine, and aneuploidy in Crassastrea gigas adults and juveniles. To evaluate the persistence of this impact, the present study focused on a sample of the same juveniles previously exposed to different atrazine treatments (0.0 1 mg/l which represents a peak value found in a polluted environment and 0.1 mg/l) for three and a half months. Then, we evaluated them for aneuploidy after another two and a half months in non polluted...
Tipo: Text Palavras-chave: Cadmium; Atrazine; Pollutant; Pacific oyster; Aneuploidy.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-2852.pdf
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Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids ArchiMer
Batista, Frederico M.; Lopez-sanmartin, Monserrat; Boudry, Pierre; Navas, Jose I.; Ruano, Francisco; Renault, Tristan; Fonseca, Vera G.; Leitao, Alexandra.
Cytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid...
Tipo: Text Palavras-chave: Aneuploidy; Ostreid herpesvirus 1; Oyster; Crassostrea.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00215/32581/35759.pdf
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Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization ArchiMer
Barranger, Audrey; Benabdelmouna, Abdellah; Degremont, Lionel; Burgeot, Thierry; Akcha, Farida.
Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban, industrial and agricultural activities. Genotoxicity is common to several families of major environmental pollutants, including pesticides, which therefore represent a potential important environmental hazard for marine organisms. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to short-term exposure to the herbicide diuron at environmental concentrations during gametogenesis. In this paper, Fluorescent in...
Tipo: Text Palavras-chave: Aneuploidy; FISH; Diuron; Crassostrea gigas.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00238/34911/33216.pdf
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Persistence of atrazine impact on aneuploidy in Pacific oysters, Crassostrea gigas ArchiMer
Bouilly, Karine; Mccombie, Helen; Leitao, Alexandra; Lapegue, Sylvie.
Widespread use of the herbicide atrazine has incited much research on its toxicity in aquatic systems, where it is routinely detected due to runoff from cultivated fields. Moreover, the determination of the genotoxic effect of such pollutants in the marine environment has become a major requirement for ecosystem protection. In the Pacific oyster, Crassostrea gigas, hypodiploid aneuploid cells have regularly been reported. There is a negative correlation between this phenomenon and growth, as well as evidence for a genetic basis. A positive relationship between atrazine and aneuploidy has previously been demonstrated in C. gigas adults and juveniles. To evaluate the persistence of this impact, our study examined the offspring of the same adult population...
Tipo: Text Palavras-chave: Aneuploidy; Crassostrea gigas; Atrazine; Toxicity; Pollutant persistence.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-580.pdf
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Pollution et taux d'aneuploïdie chez l'huître creuse Crassostrea gigas: étude expérimentale sur une population en milieu contrôlé ArchiMer
Bouilly, Karine.
Aneuploidy has previously been reported in wild and cultivated Pacific oysters, Crassostrea gigas, and it has been shown to be negatively correlated with growth. This is especially important since high variability of growth rate is one of the major problems in the aquacultural production of this species. The existence of an environmental factor (such as atrazine contamination) with an effect on aneuploidy level was first investigated through the study of a population of Crassostrea gigas subjected to different concentrations of atrazine. The treatments applied represent peak values found in the environment (0,01 mg/l) plus a value ten times higher (0,1 mg/l). Our results have shown that although atrazine did not have an effect on the mortality of adult...
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Aneuploïdie; Facteur environnemental; Atrazine; Aneuploidy; Pacific oysters; Crassostrea gigas; Environmental factor; Atrazine.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00428/53932/55060.pdf
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Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans Biol. Res.
CHIUCHETTA,SIMONE JUREMA RUGGERI; CASTRO-PRADO,MARIALBA AVEZUM ALVES DE.
Vincristine is an alkaloid widely used as an antineoplastic agent. In eukaryotic cells the drug causes blockage in the G2 phase of the cell cycle and an increase in the frequency of sister chromatid exchanges. Due to the fact that germinating Aspergillus nidulans cells spend most of their cycle in G2 phase, they provide an excellent system for the study of mitotic crossing-over. Taking into account that mitotic crossing-over occurs during G2 period, the evaluation of recombinagenic and aneugenic potential of vincristine is provided with regard to two diploid strains of A. nidulans: a wild strain (uvsH+//uvsH+) and a defective one in DNA repair (uvsH//uvsH). Drug toxicity and its effect on the asexual cycle of A. nidulans has been evaluated as well....
Tipo: Journal article Palavras-chave: Aneuploidy; Antineoplastic drug; Aspergillus nidulans; Recombinagenic activity.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100006
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