SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

CAPALBO, D. M. F.; MORAES, I. O..

Bacillus thuringiensis has highly specific insecticidal activity and holds considerable advantage over chemical controls which often have long residual action and may be toxic to nontarget organisms. In order to carry out large-scale field trials with that bioinsecticide, its sufficient local supply must be available. This study was undertaken to determine a suitable fermentation medium composition and optimal conditions for large-scale, low-cost production of B. thuringiensis. Submerged fermentation process was proposed utilizing liquid agroindustrial residues like sugar-cane molasses, coconut water, liquid residue from beer industry and the wastewater from cassava flour industry. Temperature, aeration and agitation were controlled. Analysis of pH,...

Tipo: Anais e Proceedings de eventos

Palavras-chave: Production; Insect; Pest; Antagonism; Microrganism; Bioinsecticide; Bactéria; Bacillus Thuringiensis; Biological control.

Ano: 1991

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/10809

Alternativas a fungicidas sintéticos no controle da antracnose da banana.

OLIVEIRA, E. S. DE; VIANA, F. M. P.; MARTINS, M. V. V..

Tipo: Artigo de periódico

Palavras-chave: Colletotricum musae; Fungitoxic activity; Antagonism; Post-harvest; Atividade fungitóxica.; Antagonismo; Pós-colheita..

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061843

ANTAGONISM AGAINST VIBRIO CHOLERAE BY BACTERIAL DIFFUSIBLE COMPOUND IN THE FECAL MICROBIOTA OF RODENTS

Rev. Microbiol.

Silva,Simone Helena da; Vieira,Enio Cardillo; Nicoli,Jacques Robert.

In an ex vivo agar plate assay, we monitored the appearance of an inhibitory halo against Vibrio cholerae from the feces of Wistar and Fischer rats aged 10 to 42 days. The frequency of Wistar rats showing halo increased from 0% (10 days) to a maximum of 80.0% (29 days) and then decreased to 53.3% (42 days). A similar pattern was obtained with Fischer rats but with a lower intensity (maximum frequency of 50.0% by day 36). In a separate experiment, when Wistar rats were fed a low-protein diet for 7 days, the inhibitory halo decreased drastically. Three apparently different colony morphologies were isolated from the dominant fecal microbiota: a facultative anaerobe (FAN) and two strict anaerobes (SAN). The ex vivo inhibitory test showed a halo around the...

Antagonistic activity of bacteria from the chickpea rhizosphere against Fusarium oxysporum f.sp. ciceris.

Palavras-chave: Vibrio cholerae; Escherichia coli; Streptococcus intermedius; Antagonism; Rodents.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300016

Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice

LANDA B. B.; HERVAS, A.; BETTIOL, W.; JIMENEZ-DIAZ, R.M..

The antagonistic activity against in vitro growth of Fusarium oxysporum f.sp. ciceris was determined for 74 bacterial isolates obtained from the rhizosphere of chickpeas grown in two field soils with different histories of Fusarium wilt, and for seven isolates of Pseudomonas spp. from culture collections. Twenty-four isolates of Bacillus spp. and Pseudomonas chlororaphis 30-84 showed a strong antagonism against three races (0, 1 and 5) of F.o. ciceris tested. Three selected Bacillus isolates and P. chlororaphis 30-84 were further tested against 30 isolates of races 0, 1 and 5 of F.o ciceris, races 0,1 and 2 of F.o. melonis, F.o. Phaseoli and nonpathogenic F. oxysporum. Bacillus isolates differed in their antagonistic activity and were less inhibitory to...

Tipo: Artigo de periódico

Palavras-chave: Fluorescent pseudomonas; Antagonism; Plant disease; Specificity; Suppression.; Cicer Arietinum.; Biological control; Statistical analysis..

Ano: 1997

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/12764

Vasconcelos,Ana Luiza Sampaio; Nicoli,Jacques Robert; Nardi,Regina Maria Drummond.

In the present study, production of antagonistic substances by Lactobacillus murinus against enteropathogenic bacteria was evaluated in vivo as well as a possible protective effect against an oral challenge with Salmonella Typhimurium using a gnotobiotic animal model. A higher mean survival time (P < 0.05) was observed in L. murinus-associated animals (7.89 ± 3.83 days) when compared with the control ones (4.44 ± 0.73 days). Lactobacillus murinus exerted a potent antagonistic effect in vivo against S. sonnei and to a lesser extent against S. Typhimurium as revealed by inhibitory halos around the feces of Lactobacillus-associated animals. Diameters of inhibitory halos around intestinal contents increased along the intestine tract, following...

Antibiosis of actinomycetes from Paullina cupana var. sorbilis (mart.) Ducke against fungi pathogenic.

Palavras-chave: Antagonism; Salmonella; Lactobacillus; Gnotobiotic mice.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500007

Antibiosis of Trichoderma spp strains native to northeastern Mexico against the pathogenic fungus Macrophomina phaseolina

LIOTTI, R. G.; MELLO, I. S.; PIETRO-SOUZA, W.; FIGUEIREDO, M. I. S.; SILVEIRA, B. A. V.; SILVA, F. A.; BICHARA, K. P. A.; ALMEIDA, E. G.; SILVA, G. F.; SOARES, M. A..

Guarana (Paullinia cupana var. Sorbilis (Mart.) Ducke) is a native species of economic and social importance in Brazil serving the domestic and international demands. This culture is attacked by Colletotrichum guaranicola Albuq. and Fusarium decencellulare Brick, causal agents of anthracnose and overbudding, respectively. Actinomycetes are bacteria that may produce secondary metabolites with antibacterial and antifungal potential. The isolation of endophytic with antibiosis agaist pathogenic fungi is the first step in biological control and bioprospecting antimicrobial compounds programs.

Tipo: Separatas

Palavras-chave: Antagonism; Fusarium decencellulare.; Colletotrichum Guaranicola; Fungo; Guaraná; Paullinia Cupana.; Actinobacteria..

Ano: 2015

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1029950

Mendoza,José Luis Hernández; Pérez,María Isabel Sánchez; Prieto,Juan Manuel González; Velásquez,Jesús DiCarlo Quiroz; Olivares,Jesús Gerardo García; Langarica,Homar Rene Gill.

Abstract Sampling of agricultural soils from the Mexican northeastern region was performed to detect Trichoderma spp., genetically characterize it, and assess its potential use as a biologic control agent against Macrophomina phaseolina. M. phaseolina is a phytopathogen that attacks over 500 species of cultivated plants and causes heavy losses in the regional sorghum crop. Sampling was performed immediately after sorghum or corn harvest in an area that was approximately 170 km from the Mexico-USA border. Sixteen isolates were obtained in total. Using colony morphology and sequencing the internal transcribed spacers (ITS) 1 and 4 of 18S rDNA, 14 strains were identified as Trichoderma harzianum, T. koningiopsis and T. virens. Subsequently, their antagonistic...

Antifungal and proteolytic activities of endophytic fungi isolated from Piper hispidum Sw

Palavras-chave: Macrophomina phaseolina; Antagonism; Hyperparasitism; Trichoderma.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401093

Orlandelli,Ravely Casarotti; Almeida,Tiago Tognolli de; Alberto,Raiani Nascimento; Polonio,Julio Cesar; Azevedo,João Lúcio; Pamphile,João Alencar.

Endophytes are being considered for use in biological control, and the enzymes they secrete might facilitate their initial colonization of internal plant tissues and direct interactions with microbial pathogens. Microbial proteases are also biotechnologically important products employed in bioremediation processes, cosmetics, and the pharmaceutical, photographic and food industries. In the present study, we evaluated antagonism and competitive interactions between 98 fungal endophytes and Alternaria alternata, Colletotrichum sp., Phyllosticta citricarpa and Moniliophthora perniciosa. We also examined the proteolytic activities of endophytes grown in liquid medium and conducted cup plate assays. The results showed that certain strains in the assemblage of...

Antifungal potential of crude extracts of Trichoderma spp.

Palavras-chave: Antagonism; Competitive interaction; Dual culture; Cup plate; Protease.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200359

Biota Neotropica

Marques,Eder; Martins,Irene; Mello,Sueli Correa Marques de.

Abstract Antibiosis is the mechanism by which certain microorganisms respond to the presence of others, secreting compounds or metabolites capable of inhibiting or impeding their development. The crude extract of Trichoderma contains a mixture of secondary compounds, which may show antibiotic effect, and has been used for the prospect of this fungus for biological control and other industrial purposes. Faced with the increasing demand of agriculture for ecologically compatible alternatives for the management of diseases, this work aimed to investigate the spectrum of action of Non-Volatile Metabolites (NVMs) of Trichoderma isolates against different plant pathogenic fungi. The antagonistic potential of NVMs was evaluated through the incorporation method of...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Secondary metabolites; Antagonism; Inhibition of mycelial growth; Plant pathogenic fungi.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032018000100503

Associative behaviour and antagonism of bovine rumen ciliate (Protista, Ciliophora) from Zona da Mata, Minas Gerais State, Brazil

Zoologia

D'Agosto,Marta; Guedes,Paulo Marcos da Matta.

Aiming at obtaining the community profile and verifying the occurrence of antagonism and association among ciliates, samples of rumen content were analysed in 100 bovines soon after their death. The animals were killed at the Juiz de Fora Municipal slaughter house (Juiz de Fora, Minas Gerais State, Brazil) between August 1996 and May 1997. Ciliates occurred, respectively, in the following percents and samples number: Entodinium Stein, 1859 (50,48; 100), Diplodinium Schuberg, 1888 (5,59; 98), Eudiplodinium Dogiel, 1927 (6,91; 97), Ostracodinium Dogiel, 1927 (9,68; 95), hotricha Stein, 1859 (4,15; 93), Dasytricha Schuberg, 1888 (3,31; 93), Metadi nium Awerinzew & Mutafowa, 1914 (3,06; 90), Eremoplastron Kofoid & MacLennan. 1932 (7,39; 87),...

Bioassay for selection of biocontroller bacteria against bean common blight (Xanthomonas axonopodis pv. phaseoli)

Palavras-chave: Rumen ciliate; Behaviour; Bovine; Community profile; Antagonism.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000400011

Zanatta,Zarela G.C.N.; Moura,Andréa B.; Maia,Luciano C.; Santos,André S. dos.

Emphasis has been given on selection of micro-organism for biological control. However, in order to evaluate the biological control potential of a great number of micro-organisms in a small period of time it is necessary to develop an efficient bioassay. Seven hundred and sixty bacterial isolates from different habitats, were selected for compatibility with Rhizobium leguminosarum bv. phaseoli (SEMIA 4077 e SEMIA 4080). Among them 596 isolates were ineffective against both rhizobia. Bean seeds immersed in suspension of each one of these isolates were agitated for 5 hours at 10ºC and sowed in non-sterilized soil. The plants were kept in greenhouse. After the development of cotyledonary and primary leaves, these were removed and bioassayed for Xanthomonas...

Characterization of Bacillus isolates of potato rhizosphere from andean soils of Peru and their potential PGPR characteristics

Palavras-chave: Biological control; Antagonism; Phaseolus vulgaris; Microbiolization.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300024

Calvo,Pamela; Ormeño-Orrillo,Ernesto; Martínez-Romero,Esperanza; Zúñiga,Doris.

Bacillus spp. are well known rhizosphere residents of many crops and usually show plant growth promoting (PGP) activities that include biocontrol capacity against some phytopatogenic fungi. Potato crops in the Andean Highlands of Peru face many nutritional and phytophatogenic problems that have a significant impact on production. In this context is important to investigate the natural presence of these microorganisms in the potato rhizosphere and propose a selective screening to find promising PGP strains. In this study, sixty three Bacillus strains isolated from the rhizosphere of native potato varieties growing in the Andean highlands of Peru were screened for in vitro antagonism against Rhizoctonia solani and Fusarium solani. A high prevalence (68%) of...

Control of coffee rust (Hemileia vastatrix) with Bacillus subtilis.

Palavras-chave: Bacillus; Plant growth-promoting rhizobacteria; Potato; Antagonism; Phosphate solubilization; 16S rRNA gene.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400008

Controle biológico de Bursaphelenchus cocophilus por rizobactérias e Trichoderma spp.

BETTIOL, W.; GHINI, R.; GALVAO, J. A. H..

Cell suspensions of the isolates AP-3 and AP-150 of Bacillus subtilis totally inhibited uredospores germination of the races I,II, XXV, XXIX of Hemileia vastatrix when Bacillus concentration was higher than 5 x 107 cells/ml. A higher than 70% inhibition was achieved with Bacillus concentration of 5x106 cells/ml, but the germinated uredospores showed high level of deformation. The spray of cell suspensions on coffee leaves, variety Caturra, autoclaved or not, of the isolates AP-3 and AP-150 in concentrations of 2,2x108 and 4,3x108 cells/ml, respectivelly, reduced the number of injuries in 87, 62, 73 and 72%, respectively. When those isolates were sprayed on toung plants the inhibition was 89, 67, 63 and 59%, respectively. In a field test in the 89/90...

Tipo: Anais e Proceedings de eventos

Palavras-chave: Coffee; Disease; Fungus; Rust; Antagonism; Bactéria; Hemileia Vastatrix; Bacillus subtilis; Biological control.

Ano: 1991

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/10804

Creole bean seeds microbiolization with doses of Trichoderma harzianum

SILVA, C. M. da; PINHEIRO, C. C. C.; SOUSA, I. A. L. de; LINS, P. M. P.; SILVA, G. B. da; CARVALHO, E. de A..

Tipo: Separatas

Palavras-chave: Antagonism; Anel Vermelho; Antagonismo; Nematóide; Blueberry red ringspot virus.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102784

Ciência Rural

Dalzotto,Laís; Tortelli,Brenda; Stefanski,Francine Spitza; Sacon,Deivid; Silva,Vanessa Neumann; Milanesi,Paola Mendes.

ABSTRACT: In the search for improved yields, seed treatment by microbiolization has been used as an alternative to chemical treatment. The objective was to verify the physiological and sanitary quality of creole bean seeds, var. Chumbinho, after microbiolization with doses of a commercial product (c.p.) with Trichoderma harzianum (strain ESALQ-1306). The treatments were: T1) 100 mL c.p./100 kg seeds; T2) 150 mL c.p.; T3) 200 mL c.p.; T4) 200 mL of chemical treatment (c.p., 250 g L-1 fipronil + 25 g L-1 pyraclostrobin + 225 g L-1 thiophanate-methyl); and T5) control (without coating of seeds). The tests were: sanitary test (blotter test); germination and first count; accelerated aging, cold germination without soil, speed of germination rate (SGR), seedling...

Efecto de Trichoderma y Bacillus en la dinámica poblacional de Fusarium spp. en agave tequilero (Agave tequilana Weber. var. Azul) en Jalisco.

Palavras-chave: Antagonism; Biological control; Phaseolus vulgaris L.; Vigor..

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000500151

Colegio de Postgraduados

Tlapal Bolaños, Bertha.

La marchitez es uno de los problemas fitosanitario más importantes en el agave tequilero Agave tequilana Weber var. Azul, enfermedad que esta asociada con varios microorganismos y que principalmente se presenta en plantaciones de más de 2 años de edad. Entre los posibles agentes causales de esta enfermedad se reportan a varias especies de Fusarium spp., así como a Thielaviopsis paradoxa. En el presente trabajo se planteó el de inocular con organismos benéficos plantas de agave tequilero que se usan como material propagativo en vivero para nuevas plantaciones, como parte de un manejo preventivo e integrado del cultivo. En este trabajo se estudió en vivero (Rancho El Indio, Tequila, Jalisco) y en campo, en plantaciones comerciales de agave en las...

Palavras-chave: Marchitez del agave; Fusarium spp.; Antagonismo; Supresividad; Agave wilt; Antagonism; Suppressive; Fitopatología; Doctorado.

Ano: 2013

URL: http://hdl.handle.net/10521/2117

Effects of culture filtrates of endophytic fungi obtained from Piper aduncum L. on the growth of Mycobacterium tuberculosis

Electron. J. Biotechnol.

Lima,Alita Moura de; Salem,Júlia Ignez; Souza,João Vicente Braga de; Cortez,Ana Claudia Alves; Carvalho,Clarice Maia; Chaves,Francisco Célio Maia; Veiga Junior,Valdir Florêncio da.

Substances that inhibit the growth of Mycobacterium tuberculosis could potentially be used as antibiotics. These substances could also be added to test culture media to improve the speed of tuberculosis diagnosis. The aim of this work was to investigate the influence of culture filtrates of endophytic fungi isolated from P. aduncum L. on the growth of M. tuberculosis. To achieve this objective, the following methodology was used: a) endophytic fungi were isolated from the leaves and stems of P. aduncum L.; b) the isolated fungi were submitted to submerged bioprocessing; c) culture filtrates from the bioprocess were assayed to evaluate their effect on the growth of M. tuberculosis. We isolated 315 fungal types, which represented 85 morphologies, from...

Tipo: Journal article

Palavras-chave: Antagonism; Stimulation growth; Tuberculosis.

Ano: 2011

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400008

Egg quality of hens fed different digestible lysine and arginine levels

Rev. Bras. Ciênc. Avic.

Carvalho,FB de; Stringhini,JH; Matos,MS; Café,MB; Leandro,NSM; Gomes,NA; Santana,ES; Jardim Filho,RM.

This experiment aimed at evaluating the influence of the supplementation of digestible lysine and digestible arginine at different ratios in the diet fed to layers between 24 to 44 weeks of age on egg quality. In total,320 Lohmann LSL laying hens were allotted according to a completely randomized design in a 2 x 4factorial arrangement, consisting of two digestible lysine levels (700 or 900 mg/kg of diet) and four digestible arginine levels (700, 800, 900,or 1000 mg/kg of diet). Diets contained, therefore, digestible Lys:Arg ratios of 100, 114, 128, and 142 when the diet contained 700 mg digestible lysine per kg of diet, and 78, 89, 100, and 111 when 900 mg digestible lysine per kg was supplemented. The data obtained with digestible arginine levels were...

Endophytic fungi from Brachiaria grasses in Brazil and preliminary screening of Sclerotinia sclerotiorum antagonists

Palavras-chave: Antagonism; Digestible amino acid; Egg components; Haugh units.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000100063

Scientia Agricola

Gama,Danielly de Souza; Santos,Ítalo Augusto Férrer Melo; Abreu,Lucas Magalhães de; Medeiros,Flávio Henrique Vasconcelos de; Duarte,Whasley Ferreira; Cardoso,Patrícia Gomes.

ABSTRACT: Fungal endophytes of Brachiaria, a nonhost of Sclerotinia sclerotiorum, may harbor species with antagonistic effects against this plant pathogen. The objective of this work was to investigate the diversity of endophytic fungi associated with different Brachiaria species and hybrids and evaluate their potential to inhibit the plant pathogen S. sclerotiorum. Stem samples from 39 Brachiaria spp. plants were collected in pasture fields and experimental areas of three states of Brazil resulting in 74 endophytes isolated. Twenty-eight species were identified by sequences of the Internal Transcribed Spacer (ITS) and 18S rDNA regions. Paraconiothyrium sp. was the most abundant endophyte, accounting for 24 % (14 isolates) of total, and it was isolated...