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Caractérisation morphologique et fonctionnelle des hémocytes chez l'huître plate, Ostrea edulis 5
Xue, Quiggang.
The mechanism that bivalve molluscs react to infectious agents are important research subjects in the fIeld of disease control studies for these animals. While the selection of disease resistant populations have made decisive progression in some species, the mechanism behinds these resistant phenomena is not yet clear so far. However, hemocytes must represent one of the key factors in the reaction process. Based on this hypothesis, the structure and function of European flat oyster, Ostrea edulis, hemocytes have been investigated in aid of different techniques in the present study. ln addition, Pacific oyster, Crassostrea gigas, hemocytes have been analysed in certain aspects so as to provide some results for comparison. European flat oyster and Pacific...
Tipo: Text Palavras-chave: Bivalvia; Ostrea edulis; Crassostrea gigas; Mécanismes de défense; Hémocytes; Granulocytes; Hyalinocytes; Séparation; Anticorps monoclonaux; Enzymes; Cytométrie de flux; Bivalvia; Ostrea edulis; Crassostrea gigas; Defence mechanisms; Hemocytes; Granulocytes; Hyalinocytes; Separation; Monoclonal antibodies; Enzymes; Flow cytometry.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00107/21820/19406.pdf
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Contribution à l'étude du système neuroendocrine de la moule Mytilus edulis (Mollusca: Bivalvia) 5
Kellner-cousin, Kristell.
Dans le cadre de ce travail, l'étude du système neuroendocrine de la moule Mytilus edulis a été envisagée sous plusieurs aspects qui définissent les quatre grandes parties de la thèse. Dans une première partie est décrit le travail de préparation et de caractérisation d'anticorps monoclonaux (AcMc) spécifiques de ganglions cérébroïdes (GC) de Mytilus edulis. Le développement de techniques de préparation d'AcMc dirigés contre des antigènes non purifiés a en effet permis de produire un ensemble de réactifs de type AcMc dirigés contre différents antigènes issus d'un broyat de GC de moule. La spécificité de ces AcMc a été étudiée en microscopie optique puis électronique selon des techniques immunocytochimiques (immunopéroxydase indirecte et immunogold). La...
Tipo: Text Palavras-chave: Mollusque; Bivalve; Mytilus edulis; Cellules neurosécrétrices; Système nerveux; Système immunitaire; Controle de la croissance; Insuline; Anticorps monoclonaux; Immunocytocbimie; ELISA; PCR; Banque d'ADNc.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00109/22019/19642.pdf
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Etude d'une prolifération hémocytaire chez la moule Mytilus edulis 5
Noel, Danièle.
Hemic proliferative disease in the bay mussel, Mytilus edulis, has been studied following mortalities in Washington State (USA). The disease has been reproduced in the labortory. The establishment of this experimental model led to study tumoral characteristics of the atypic hemocytes : ultrastrure, in vitro cultivation, transplantation. The infectious etiology of this tumor has been verified. It has been hypothesized to result from a viral infection since retrovirus-like particules have been observed following induction by bromodeoxyuridine. Specific monoclonal antibodies against atypic hemocytes have been obtained and characcterized
Tipo: Text Palavras-chave: Mytilus; Mollusque; Hémocyte; Tumeur; Ulstrastructure; In vitro culture; Transplantation; Rétrovirus; Anticorps monoclonaux; Mytilus; Mollusca; Hemocyte; Tumor; Ultrastructure; In vitro cultivation; Transplantation; Retrovirus; Monoclonal antibodies.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00428/53930/55057.pdf
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