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Registros recuperados: 13
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A preliminary survey of M. hyopneumoniae virulence factors based on comparative genomic analysis Genet. Mol. Biol.
Ferreira,Henrique Bunselmeyer; Castro,Luiza Amaral de.
Mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia (PEP), a major problem for the pig industry. The mechanisms of M. hyopneumoniae pathogenicity allow to predict the existence of several classes of virulence factors, whose study has been essentially restricted to the characterization of adhesion-related and major antigenic proteins. The now available complete sequences of the genomes of two pathogenic and one non-pathogenic strain of M. hyopneumoniae allowed to use a comparative genomics approach to putatively identify virulence genes. In this preliminary survey, we were able to identify 118 CDSs encoding putative virulence factors, based on specific criteria ranging from predicted cell surface location or variation between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Porcine enzootic pneumoniae; Virulence; Host-pathogen interaction; Antigen; Adhesin.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200012
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Antigenic Relationship among Kinetoplastid Flagellates : Herpetomonas, Leishmania and Bodo OAK
De, A.; Das, Pradeep; Bhattacharya, Amal; Das, D..
Palavras-chave: Antigen; Antibody; Immunoreactivity; Kinetoplastid flagellates.
Ano: 1994 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/215
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Catalase activity of different Candida species after exposition to specific antiserum BJM
Miyasaka,Natália R.S.; Unterkircher,Carmelinda S.; Shimizu,Mario T..
Antisera were developed in rabbits after challenge with intracellular antigens of Candida albicans, C. tropicalis and C. parapsilosis. Microorganism catalase has been correlated with virulence, resistance to drugs and immunogenicity. The intracellular catalase is consistently present in strains of Candida and in this paper, the enzyme activity was analysed by PAGE after exposition to antisera. The catalases of C. albicans, C. parapsilosis and C. tropicalis were immunogenic and differed in their binding to specific antibodies raised in rabbits. Tests of cross-reactivity between different Candida species showed that when antiserum from C. albicans immunized rabbit was incubated with intracellular extracts of these three Candida species, the catalases...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidiasis; Catalase; Candida; Antigen; Diagnosis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100009
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Comparison of predicted binders in Rhipicephalus (Boophilus) microplus intestine protein variants BM86 Campo Grande Strain, BM86 and BM95. Repositório Alice
ANDREOTTI, R.; PEDROSO, M. S.; CAETANO, A. R.; MARTINS, N. F..
This paper reports the sequence analysis of Bm86 Campo Grande strain comparing it with Bm86 and Bm95 antigens from the preparations TickGardPLUS and GavacTM, respectively. The PCR product was cloned into pMOSBlue and sequenced. The secondary structure prediction tool PSIPRED was used to calculate alpha helices and beta strand contents of the predicted polypeptide. The hydrophobicity profile was calculated using the algorithms from the Hopp and Woods method, in addition to identification of potential MHC class-I binding regions in the antigens. Pair-wise alignment revealed that the similarity between Bm86 Campo Grande strain and Bm86 is 0.2% higher than that between Bm86 Campo Grande strain and Bm95 antigens. The identities were 96.5% and 96.3%...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Rhipicephalus (Boophilus) microplus; Bm86; Bm95; Tick; Antigen; Antígeno; Carrapato; Imunologia; Immunology.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/190432
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Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil BJID
Szargiki,Regiane; Castro,Edilene Alcântara de; Luz,Ennio; Kowalthuk,Wolodymir; Machado,Ângela Maria; Thomaz-Soccol,Vanete.
We evaluated the effectiveness of serological and parasitological methods for cutaneous leishmaniasis (CL) diagnosis in patients from the central region of Paraná state, southern Brazil. Five groups were compared: clinical diagnosis, parasitological diagnosis, communicants, inhabitants of a non-endemic area and carriers of other etiologies. Two antigens were prepared from promastigotes of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis for indirect immunofluorescence assay, ELISA and immunoblotting. The parasitological approaches detected 79.3% of the patients with a clinical diagnosis; the parasites were identified by PCR as L. (V.) braziliensis. Serological methods showed 95% sensitivity for homologous antigens. Immunoblotting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Immunodiagnosis; Antigen; Western blotting.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100011
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Expression and purification of the non-tagged LipL32 of pathogenic Leptospira BJMBR
Hauk,P.; Carvalho,E.; Ho,P.L..
Leptospirosis is a reemerging infectious disease and the most disseminated zoonosis worldwide. A leptospiral surface protein, LipL32, only occurs in pathogenic Leptospira, and is the most abundant protein on the bacterial surface, being described as an important factor in host immunogenic response and also in bacterial infection. We describe here an alternative and simple purification protocol for non-tagged recombinant LipL32. The recombinant LipL32(21-272) was expressed in Escherichia coli without His-tag or any other tag used to facilitate recombinant protein purification. The recombinant protein was expressed in the soluble form, and the purification was based on ion exchange (anionic and cationic) and hydrophobic interactions. The final purification...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antigen; LipL32; Pathogenic Leptospira; Non-tagged protein purification; Diagnosis; Vaccine.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400005
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Expression of the Mycobacterium bovis P36 gene in Mycobacterium smegmatis and the baculovirus/insect cell system BJMBR
Bigi,F.; Taboga,O.; Romano,M.I.; Alito,A.; Fisanotti,J.C.; Cataldi,A.A..
In the present study we evaluated different systems for the expression of mycobacterial antigen P36 secreted by Mycobacterium bovis. P36 was detected by Western blot using a specific antiserum. The P36 gene was initially expressed in E. coli, under the control of the T7 promoter, but severe proteolysis prevented its purification. We then tried to express P36 in M. smegmatis and insect cells. For M. smegmatis, we used three different plasmid vectors differing in copy number and in the presence of a promoter for expression of heterologous proteins. P36 was detected in the cell extract and culture supernatant in both expression systems and was recognized by sera from M. bovis-infected cattle. To compare the expression level and compartmentalization, the MPB70...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis; Mycobacterium smegmatis; Insect cells; Baculovirus; Secreted proteins; P36; MPB70; Antigen.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100004
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FISH IMMUNE SYSTEM Gayana
Olabuenaga,Susana E..
The defence against infections is the essential function of the immune system of all Vertebrates. Fish present an efficient and developed immunologic response and in the case of Teleosts, with some similarities to the higher vertebrates. It can be divided into two types: innate or non specific and adquired or specific, both with a humoral and cellular compartments. Their relative importance can change according to the age of fish and is always under the influence of different factors. The most relevant components are mentioned here. There are two factors in fishes which strongly influence the immune response and antibody production, seasonal changes and temperature. The main lymphoid organs are described here: the thymus, the kidney and the spleen, as well...
Tipo: Journal article Palavras-chave: Antigen; Antibody; Leukocytes; Phagocytosis; Lymphokines; Inflammation; Immunostimulants.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200010
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Identificação de predadores de Orphulella punctata (de Geer) (Orthoptera, Acrididae) através da serologia Zoologia
Cassaro-Silva,Marta; Serrão,José Eduardo; Sousa-Silva,Carlos; Marques-Pacheco,Josué.
Females of Orphulella punctata (de Geer, 1794) were captured in the field and kept in cages with food and boxes filled with moist soil for oviposition. The eggs, macerated with 0,85% saline solution and centrifugated, were used as the immunizing antigen for obtention of the specific antiserum (AS-O). Rabbits were immunized by the linphonodule injection method with two injections of this antigen, with an interval of 15 days between the 1st and 2nd inoculations. Serological tests were performed using double diffusion in agar gel with homologous and heterologous antigens. Specific serological reactions were obtained 14 days after the 1st inoculation. The antiserum of the 30th day gave 5, 2-4 and 1-4 precipitation lines respectively with eggs, female and male...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Arthropod; Grasshopper; Antiserum; Antigen; Biological control; Predation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000100007
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Isolamento e identificação de proteínas em leptospira. Repositório Alice
ALVES, F. S. F.; LEFEBVRE, R.; PROBERT, W..
1994
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Leptospira; Eletroforese; Antigeno; Protein; Eletrophorese; Antigen; Proteína; Eletroforese; Antígeno; Antigens; Doença animal; Leptospirose; Leptospira; Antigen; Protein; Animal diseases; Infectious diseases; Leptospirosis.
Ano: 1994 URL: http://www.alice.cnptia.embrapa.br/handle/doc/514477
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Oral tolerance induction with altered forms of ovalbumin BJMBR
Stransky,B.; Faria,A.M.C.; Vaz,N.M..
As a T cell-dependent phenomenon, oral tolerance is not expected to depend necessarily on native configuration of antigens. We investigated the induction of oral tolerance with modified ovalbumin (Ova). Oral administration of heat-denatured (HD-Ova) and cyanogen bromide-degraded ovalbumin was less effective than native Ova in inducing oral tolerance in B6D2F1 mice. HD-Ova was effective in suppressing delayed-type hypersensitivity (DTH) reactions but did not suppress specific antibody formation. Injection of Ova directly into the stomach, but not into the ileum or cecum, suppressed subsequent immunization to DTH reactions. Gavage with protease inhibitors (aprotinin or ovomucoid) before gavage with Ova was ineffective in blocking tolerance induction....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antigen; Digestion; Oral tolerance; Mouse; Ovalbumin.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300009
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Produção de antígeno e separação da proteína P28 através de microfiltragem seriada para sorodiagnóstico da artrite encefalite caprina. Repositório Alice
ALVES, L. A. de O..
Resumo: O vírus da Artrite Encefalite Caprina (CAEV) é um lentivírus que causa uma enfermidade crônica que não possui tratamento ou vacinação como ferramenta de controle, sendo responsável por perdas econômicas significativas em todo mundo. O diagnóstico é principalmente baseado em exames sorológicos, onde os antígenos utilizados para o diagnóstico são compostos basicamente pelas proteínas estruturais p28 e gp135. Este estudo teve como objetivo produzir um antígeno (Ag) a partir de cultura de células de Membrana Sinovial Caprina (MSC) infectadas com CAEV, pela técnica de microfiltração seriada, substituindo a ultracentrifugação em colchão de sacarose ( Ag UCCS) para utilização em um ELISA indireto (ELISA-i). Amostras de 188 soros caprinos, que previamente...
Tipo: Tese/dissertação (ALICE) Palavras-chave: Artrite encefalite caprina; Lentivirose; ELISA; Caprino; Ovino; Doença animal; Lentivírus; Maedi-Visna; Antígeno; Vírus da Artrite Encefalite Caprina; Maedi-Visna; CAEV; Antigen; Caprine arthritis encephalitis virus.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/928298
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Teste de pele em caprinos vacinados e infectados com Corynebacterium pseudotuberculosis. Repositório Alice
ALVES, F.S.F.; OLANDER, H.J..
Dez caprinos foram vacinados com toxoide a 3%, outros dez com uma bacterina e mais dois grupos-controle de cinco animais cada, submetidos a inoculacao de infusao de cerebro e coracao e solucao salina, respectivamente. Todos os animais foram examinados e avaliados com um teste de pele. Tanto o toxoide quanto a bacterina foram produzidos a partir de amostra de Corynebacterium pseudotuberculosis. Todos os caprinos foram desafiados com C. pseudotuberculosis, trinta dias apos as vacinacoes. Nenhuma das vacinas induziu reacao de hipersensibilidade na pele dos caprinos antes do desafio. Apos o desafio, todos os animais desenvolveram reacoes mensuraveis na primeira, quinta e decima semana em resposta ao teste de pele. Os diametros da reacao dermica aumentaram do...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Diagnostico; Teste intradermico; Imunidade celular; Antigeno; Doenca subclinica; Diagnostic; Intradermic test; Cell immunity; Antigen; Subclinical disease.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/99152
Registros recuperados: 13
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