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| Barnabe, G.; Tournamille, J.-c.. |
| Mature eggs were expressed from captured female and fertilised artificially with slightly bloody sperm, in dry conditions or in a humid atmosphere, both at 13 degree C. The best incubation conditions proved to be 13 degree C, with streptomycin and penicillin. Another sample was kept at 18 degree C. Hatching occurred after 4 or 5 days, larvae showing positive phototropism. The system of renewal of water and artificial food is described. The embryonic reserve drop of lipid disappeared between day 14 and 16. Observations on larvae were like previous ones, but live larvae had larger mouths than had fixed specimens, and it was found that temperatures of 13 degree C or 18 degree C were suitable beyond the egg stage. A sudden change in temperature caused by a... |
| Tipo: Text |
Palavras-chave: Temperature; Sperm; Eggs; Dicentrarchus labrax; Artificial reproduction. |
| Ano: 1972 |
URL: http://archimer.ifremer.fr/doc/1972/publication-2063.pdf |
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| Kaspar, Vojtech; Hulak, Martin; Kohlmann, Klaus; Vandeputte, Marc; Rodina, Marek; Gela, David; Linhart, Otomar. |
| Sperm competition occurs when sperm from different males compete for fertilization. The aim of this in vitro fertilization study was to compare the competitive success of five males using heterosperm with an equal number of sperm from each male and four different sperm/egg ratios (5,000, 10,000, 20,000 and 100,000 spermatozoa per egg) in order to better understand variations of competitive fertilization. The roles of sperm motility and velocity were studied. Fertilization and hatching rates of the 5 males studied, with 100,000 spermatozoa per egg, were between 23.7-94.8% and 23.7-92.2%, respectively. Sperm velocity and percentage of motile sperm ranged from 85.0 to 137.6 mu m s(-1) and 2.0 to 93.5% at 15 see post sperm activation, respectively. The... |
| Tipo: Text |
Palavras-chave: Paternity assignment; Artificial reproduction; Aquaculture. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6551.pdf |
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| Santos,Hélio Batista dos; Sampaio,Edson Vieira; Arantes,Fábio Pereira; Sato,Yoshimi. |
| Lophiosilurus alexandri is an endemic fish from the São Francisco River basin, Brazil. The aim of this study was to induce L. alexandri to spawn and to obtain data on several reproductive variables for this species. For induced spawning, adults were submitted to Cyprinus carpio pituitary homogenate (CPH). Nine of the 12 females (75%) responded positively to the treatment. The stripping of oocytes was performed 8.4 h after the second dose of CPH with the water temperature maintained at 26ºC. The number of stripped oocytes per gram of ova was 74 ± 5 oocytes g-1, and the mean oocyte diameter was 3.1 ± 0.2 and 3.6 ± 0.2 mm, before and after hydration, respectively. The oocytes were opaque, yellowish, demersal, highly adhesive, and covered by a gelatinous coat.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artificial reproduction; Fecundity; Fertility; Hypophysation assay; Neotropical catfish. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300607 |
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| Sanches,Eduardo Antônio; Caneppele,Danilo; Okawara,Renan Yoshiharu; Damasceno,Danielle Zanerato; Bombardelli,Robie Allan; Romagosa,Elizabeth. |
| Abstract The Steindachneridion parahybae is an endangered catfish from Brazil and strategies applied for gametes optimization are necessary. The aim of this study was to assess inseminating doses and water volume upon the fertilization, hatching rates and percentage of normal larvae in S. parahybae . Was used a randomized design in factorial scheme (4×4) with four inseminating doses: 1.0×104, 1.0×105, 1.0×106, 1.0×107spermatozoa oocyte-1 and four volumes of water: 1, 35, 65 and 95mL of water g-1 of oocytes. The combination of doses and volumes were performed in triplicates (n=48). Each incubator (1.5L of useful volume) with 1g of oocytes was considered as an experimental unit. Significant interaction between inseminating doses and volumes of water to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artificial reproduction; Freshwater fish; Oocytes; Sperm; Surubim-do-Paraíba. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000100204 |
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