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Registros recuperados: 9
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Assessment of the metabolism of different strains of Bacillus megaterium BABT
Suarez,Carlos Alberto Galeano; Montano,Inti Doraci Cavalcanti; Nucci,Edson Romano; Iemma,Monica Rosas da Costa; Giordano,Raquel de Lima Camargo; Giordano,Roberto de Campos.
Bacillus megaterium is a promising host for expression of heterologous proteins. This paper reports the nutrient consumption patterns and production of metabolites for three different strains of B. megaterium, ATCC 14945, QMB 1551 and PV 361, which is QMB 1551 with seven constitutive plasmids deleted. 14 h cultivations in agitated flasks were run, for two different media: A (LB plus 10g/L glucose) and B (medium A, with the yeast extract replaced by tryptone). Strains PV361 and QMB 1551 showed higher maximum specific growth rates in medium B, reaching 0.42 h-1 and 0.48 h-1 respectively. The main by-products of the glucose overflow mechanism were acetate and lactate, for all three strains, which had preferential amino acids for substrate: Ala, Asp, Glu, Ser....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metabolic profile; Bacillus megaterium; Culture media.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400001
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Bioconversion of cassava starch by-product into Bacillus and related bacteria polyhydroxyalkanoates Electron. J. Biotechnol.
Krueger,Cristhiane L; Radetski,Claudemir M; Bendia,Amanda G; Oliveira,Ida M; Castro-Silva,Marcus A; Rambo,Carlos R; Antonio,Regina V; Lima,André O.S..
Background: Unlike petroleum-based synthetic plastics, biodegradable biopolymer generation from industrial residue is a key strategy to reduce costs in the production process, as well as in the waste management, since efficient industrial wastewater treatment could be costly. In this context, the present work describes the prospection and use of bacterial strains capable to bioconvert cassava starch by-product into biodegradable polyhydroxyalkanoates (PHAs). Results: The first step of this study was the bacterial competence screening which was conducted with 72 strains covering 21 Bacillus and related species. The microorganism growth in a medium with a starch substrate was measured by an innovative MTT assay, while the ability of the bacteria to secrete...
Tipo: Journal article Palavras-chave: Bacillus megaterium; Biodegradable plastics; Biopolymer; MTT assay; Starch substrates.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300008
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Characterization of the penicillin G acylase from Bacillus megaterium ATCC 14945 BABT
Souza,Vanessa Ribeiro de; Silva,Ana C. G.; Pinotti,Laura Marina; Araújo,Heloísa Sobreiro Selistre; Giordano,Raquel de Lima Camargo.
The purpose of this work was to characterize the enzyme penicillin G acylase (PGA) produced by Bacillus megaterium. Purification of the enzyme by ultra/diafiltration did not allow the detection of the PGA band by SDS-PAGE electrophoresis due to the high content of remaining proteins. However, using the DNA of the microorganism, it was possible to replicate the genes of the two B. megaterium PGA reported in literature, showing that the enzyme consisted of two sub-units, having 245 and 537 amino acids each and an average molecular mass of 26950 and 59070 Da, respectively. The parameters studied were: 1) the influence of temperature in the 25-60(0)C range, 2) pH in the 5-10 range and 3) substrate concentration, this was tested to obtain results on the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Penicillin G acylase; Bacillus megaterium; Characterization.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400013
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Comparative study on the biodegradation of chlorpyrifos-methyl by Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6 Anais da ABC (AABC)
ZHU,JIANGWEI; ZHAO,YAN; RUAN,HONGHUA.
Abstract: The strains CM-Z19 and CM-Z6, which are capable of highly degrading chlorpyrifos-methyl, were isolated from soil. They were identified as Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6, respectively, based on the 16S rRNA and an analysis of their morphological, physiological and biochemical characteristics. The strain CM-Z19 showed 92.6% degradation of chlorpyrifos-methyl (100 mg/L) within 5 days of incubation, and the strain CM-Z6 was 99.1% under the same conditions. In addition, the degradation characteristics of the two strains were compared and studied, and the results showed that the strain CM-Z19 had higher phosphoesterase activity and ability to degrade the organophosphorus pesticide than did the strain CM-Z6. However, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus megaterium; Pseudomonas syringae; Chlorpyrifos-methyl; 3; 5; 6-trichloro-2-pyridinol; Biodegradation.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500624
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Lipopeptides produced by a soil Bacillus megaterium strain. Repositório Alice
PUEYO, M. T.; BLOCH JUNIOR, C.; CARMONA RIBEIRO, A. M.; MASCIO, P. di..
bitstream/item/177855/1/SP-19614-ID-30897.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Microorganism; Lipopeptide; Microrganismo; Bacillus megaterium.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/190620
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Primer universal para leveduras não detecta a presença de Bacillus megaterium. Repositório Alice
NALIN, R.; AUGUSTINI, B. C.; VALENTE, P.; BERNARDI, T. L.; SILVA, G. A. da.
2011
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: PCR; Bacillus megaterium; Microbiologia; Levedura; Metodologia; Identificação; Microrganismo.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/908311
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Rizobactérias no crescimento e na produtividade da cebola Ciência Rural
Harthmann,Oscar Emilio Ludtke; Mógor,Átila Francisco; Wordell Filho,João Américo; Luz,Wilmar Cório da.
A associação de plantas com rizobactérias pode promover o crescimento vegetal, reduzindo custos de produção. As rizobactérias benéficas podem ser uma alternativa para a produção de cebola. Neste trabalho, foi avaliado o efeito da aplicação de rizobactérias no crescimento e na produtividade da cebola da cultivar 'Bola Precoce'. A aplicação foi realizada na Estação Experimental da Epagri, em Ituporanga, Santa Catarina (SC), no ano de 2008. O delineamento experimental foi em blocos ao acaso, com cinco repetições, sendo utilizadas as rizobactérias Pseudomonas spp. W6, Bacillus megaterium W19 e Bacillus cereus UFV40, microbiolizadas isoladamente nas sementes ou em mistura, juntamente com uma testemunha não tratada. Os tratamentos com aplicação de rizobactérias...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa; Bacillus megaterium; Promoção de crescimento.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000200033
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Sugarcane inoculated with endophytic diazotrophic bacteria: effects on yield, biological nitrogen fixation and industrial characteristics Anais da ABC (AABC)
ANTUNES,JADSON EMANUEL L.; FREITAS,ANA DOLORES S. DE; OLIVEIRA,LOUISE M.S.; LYRA,MARIA DO CARMO C.P. DE; FONSECA,MANOEL A.C.; SANTOS,CAROLINA E.R.S.; OLIVEIRA,JOSÉ DE PAULA; ARAÚJO,ADEMIR SÉRGIO F. DE; FIGUEIREDO,MÁRCIA V.B..
Abstract: The growing need for the use of clean energy has led society to seek alternatives to fossil fuels in order to less pollute the environment. Sugarcane has been known to produce enough biomass and to have associative microorganisms, such as plant growth-promoting bacteria (PGPB), which have the potential to improve the yield of crops. The objective of this study is to evaluate the efficiency and the isotopic dilution of 15N of microbial inoculants containing endophytic diazotrophic bacteria, individually inoculated and in mixture, on productivity, technological quality and biological nitrogen fixation (BNF) of sugarcane variety RB92579. The experiment was conducted for three consecutive years, comprising different crop cycles, in a completely...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus megaterium; Herbaspirillum seropecicae; Microbial inoculant; Natural abundance of 15N; Pseudomonas sp.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700863
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The nematicidal effect of some bacterial biofertilizers on Meloidogyne incognita in sandy soil BJM
El-Hadad,M.E; Mustafa,M.I; Selim,Sh.M; El-Tayeb,T.S; Mahgoob,A.E.A; Aziz,Norhan H. Abdel.
In a greenhouse experiment, the nematicidal effect of some bacterial biofertilizers including the nitrogen fixing bacteria (NFB) Paenibacillus polymyxa (four strains), the phosphate solubilizing bacteria (PSB) Bacillus megaterium (three strains) and the potassium solubilizing bacteria (KSB) B. circulans (three strains) were evaluated individually on tomato plants infested with the root-knot nematode Meloidogyne incognita in potted sandy soil. Comparing with the uninoculated nematode-infested control, the inoculation with P. polymyxa NFB7, B. megaterium PSB2 and B. circulans KSB2, increased the counts of total bacteria and total bacterial spores in plants potted soil from 1.2 to 2.6 folds estimated 60 days post-inoculation. Consequently, the inoculation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paenibacillus polymyxa; Bacillus megaterium; B. circulans; Biofertilizers; Biocontrol; Rootknot nematodes; Meloidogyne incognita.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100014
Registros recuperados: 9
Primeira ... 1 ... Última
 

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