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Registros recuperados: 16
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Análise da viabilidade comercial de produtos à base de Bacillus subtilis e Bacillus pumilus para o controle de fitopatógenos no Brasil. Repositório Alice
D'AGOSTINO, F.; MORANDI, M. A. B..
2009
Tipo: Capítulo em livro científico (ALICE) Palavras-chave: Bacillus subtilis; Bacillus pumilus; Doença de planta; Controle biológico; Bactéria não-patogênica; Plant diseases and disorders; Biological control; Biological control agents.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/580913
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Bacterial spot and early blight biocontrol by epiphytic bacteria in tomato plants. Repositório Alice
LANNA FILHO, R.; ROMEIRO, R. da S.; ALVES, E..
Abstract ? The objective of this work was to evaluate in vitro and in vivo biocontrol of Xanthomonas vesicatoria) Alternaria solani) by the epiphytic bacteria Paenibacillus macerans and Bacillus pumilus. Tomato plants were previously sprayed with epiphytic bacteria, benzalkonium chloride and PBS buffer and, after four days, they were inoculated with A. solani and X. vesicatoria. To determine the phytopathogenic bacteria population, leaflet samples were collected from each treatment every 24 hours, for seven days, and plated on semi-selective medium. The effect of epiphytic bacteria over phytopathogens was performed by the antibiosis test and antagonistic activity measured by inhibition zone diameter. The epiphytic and benzalkonium chloride drastically...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Alternaria solani; Bacillus pumilus; Paenibacillus macerans; Xanthomonas vesicatoria; Biological control; Epiphytic bacteria.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/883671
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Bacterial spot and early blight biocontrol by epiphytic bacteria in tomato plants PAB
Lanna Filho,Roberto; Romeiro,Reginaldo da Silva; Alves,Eduardo.
The objective of this work was to evaluate in vitro and in vivo biocontrol of bacterial spot (Xanthomonas vesicatoria) and early blight (Alternaria solani) by the epiphytic bacteria Paenibacillus macerans and Bacillus pumilus. Tomato plants were previously sprayed with epiphytic bacteria, benzalkonium chloride and PBS buffer and, after four days, they were inoculated with A. solani and X. vesicatoria. To determine the phytopathogenic bacteria population, leaflet samples were collected from each treatment every 24 hours, for seven days, and plated on semi-selective medium. The effect of epiphytic bacteria over phytopathogens was performed by the antibiosis test and antagonistic activity measured by inhibition zone diameter. The epiphytic and benzalkonium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternaria solani; Bacillus pumilus; Paenibacillus macerans; Xanthomonas vesicatoria; Biological control; Epiphytic bacteria.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001200007
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Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India BJM
Parvathi,Ammini; Krishna,Kiran; Jose,Jiya; Joseph,Neetha; Nair,Santha.
Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus pumilus; FAME; 16S rDNA; CesB; AP-PCR.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200012
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BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG¯CTC-3' BJM
Chies,Jocelei M.; Dias,Ana C. de O.; Maia,Hélio M.M.; Astolfi-Filho,Spartaco.
A strain of Bacillus pumilus was isolated and identified from water samples collected from a small affluent of the Amazon River. Type II restriction endonuclease activity was detected in these bacteria. The enzyme was purified and the molecular weight of the native protein estimated by gel filtration and SDS-PAGE. The optimum pH, temperature and salt requirements were determined. Quality control assays showed the complete absence of "nonspecific nucleases." Restriction cleavage analysis and DNA sequencing of restriction fragments allowed the unequivocal demonstration of 5´GAG<FONT FACE=Symbol>¯</FONT>CTC3´ as the recognition sequence. This enzyme was named BpuAmI and is apparently a neoschizomer of the prototype restriction endonuclease SacI....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Type II restriction endonuclease; BpuAmI; SacI; Neoschizomers; Bacillus pumilus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100018
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Draft genome sequence of Bacillus pumilus CCMA-560, isolated from an oil-contaminated mangrove swamp. Repositório Alice
DOMINGOS, D. F.; DELLAGNEZZE, B. M.; GREENFIELD, P.; REYES, L. F.; MELO, I. S. de; MIDGLEY, D. J.; OLIVEIRA, V. M. de.
Abstract: Bacillus pumilus strain CCMA-560 was isolated from an oil-contaminated mangrove swamp and was shown to produce biosurfactants. The strain appears to be capable of degrading some plant cell wall-related compounds, including hemicelluose and pectin. Genes for biopolymer export and polysaccharide intercellular adhesin synthesis were also annotated.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bacillus pumilus.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/974503
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Efeitos da antibiose e inibição de germinação por Bacillus pumilus e Bacillus subtilis em Thielaviopsis paradoxa. Repositório Alice
VENTURINI, G.; BRANDI, F.; BETTIOL, W..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Antibiose; Bacillus pumilus; Thielaviopsis paradoxa; Bacillus subtilis.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1010912
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Eficiência de biofungicidas no controle de doenças fúngicas em melão. Repositório Alice
GONZAGA, A. de M.; CASTRO, G. H. M.; OSTER, A. H.; VIANA, F. M. P..
2009
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Bacillus subtillis; Bacillus pumilus; 'Gold Mine'.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/575291
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Inibição do crescimento micelial e da germinação de esporos de Thielaviopsis paradoxa por Bacillus subtilis e Bacillus pumilus. Repositório Alice
VENTURINI, G.; BRANDI, F.; BETTIOL, W..
O objetivo do trabalho foi avaliar os efeitos de Bacillus pumilus (QST 2808) e Bacillus subtilis (QST 713) na inibição da germinação de esporos e no crescimento micelial de Thielaviopsis paradoxa, agente causal da podridão abacaxi da cana-de-açúcar. O primeiro teste avaliou a taxa de crescimento micelial do patógeno em placas de Petri, totalizando 15 placas em três tratamentos (inoculação simultânea; 24 horas e 48 horas após o bioagente). Em seguida avaliou o crescimento micelial na presença dos metabólitos produzidos pelos agentes de biocontrole, adicionados ao meio batata-dextrose-ágar, em cinco concentrações (10%; 1%; 0,1%; 0,01%; 0,001%). No teste de germinação avaliou-se o porcentual de germinação dos conídios na presença dos agentes de biocontrole...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Agentes de biocontrole; Antagonismo; Agentes de biocontrole; Podridão; Cana de açúcar; Doença de planta; Plant diseases and disorders; Plant rots; Ceratocystis paradoxa; Biological control; Bacillus subtilis; Bacillus pumilus.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1006490
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Isolamento, seleção de bactérias e efeito de Bacillus spp. na produção de mudas orgânicas de alface Horticultura Brasileira
Gomes,Andréa M.A.; Mariano,Rosa L.R.; Silveira,Elineide B.; Mesquita,Júlio C.P..
Isolados bacterianos epifíticos e endofíticos, obtidos de plantas sadias de alface, foram avaliados para promoção de crescimento de mudas e plantas, respectivamente em estufa e campo de cultivo orgânico (Chã Grande-PE). Nos experimentos em estufa, foi utilizada a cultivar Verônica e em campo, as cultivares Verdinha e Verônica. Os isolados foram aplicados por bacterização simultânea nas sementes e substrato. Em campo, foram utilizados os isolados mais eficientes, C25 (Bacillus thuringiensis subvar. kenyae) e C116 (Bacillus pumilus), separadamente e em mistura, após teste de compatibilidade. Em estufa, foram avaliadas a matéria fresca de raízes (MFR), da parte aérea (MFPA) e total (MFT), 21 dias após a bacterização. Em campo, foi determinado o peso da...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactuca sativa; Bacillus pumilus; Bacillus thuringiensis subvar; Kenyae; Bactérias promotoras de crescimento de plantas; Mecanismos de ação; Sistema orgânico.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362003000400026
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Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2 BJM
Sangeetha,R.; Arulpandi,I.; Geetha,A..
Proteolysis-resistant lipases can be well exploited by industrial processes which employ both lipase and protease as biocatalysts. A proteolysis resistant lipase from Bacillus pumilus SG2 was isolated, purified and characterized earlier. The lipase was resistant to native and commercial proteases. In the present work, we have characterized the lip gene which encodes the proteolysis-resistant lipase from Bacillus pumilus SG2. The parameters and structural details of lipase were analysed. The lip gene consisted of 650 bp. The experimental molecular weight of SG2 lipase was nearly double that of its theoretical molecular weight, thus suggesting the existence of the functional lipase as a covalent dimer. The proteolytic cleavage sites of the lipase would have...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus pumilus; Covalent dimer; Lipase; Proteolytic-resistance.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200004
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Properties of a biosurfactant produced by Bacillus pumilus using vinasse and waste frying oil as alternative carbon sources BABT
Oliveira,Juliana Guerra de; Garcia-Cruz,Crispin Humberto.
Biosurfactants are chemical molecules produced by the microorganisms with potential for application in various industrial and environmental sectors. The production parameters and the physicochemical properties of a biosurfactant synthesized by Bacillus pumilus using different concentrations of vinasse and waste frying oil as alternative carbon sources were analyzed. The microorganism was able to grow and produce a biosurfactant using both the residues. The surface tension was reduced up to 45 mN/m and the maximum production of crude biosurfactant was 27.7 and 5.7 g/l for vinasse and waste frying oil, respectively, in concentration of 5%. The critical micelle concentration (CMC) results of 1.5 and 0.2 g/l showed the efficiency of the biosurfactant produced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosurfactants; Vinasse; Waste frying oil; Bacillus pumilus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100020
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Purification and Partial characterization of manganese peroxidase from Bacillus pumilus AND Paenibacillus sp. BJM
Oliveira,Patrícia Lopes de; Duarte,Marta Cristina Teixeira; Ponezi,Alexandre Nunes; Durrant,Lúcia Regina.
The production of manganese peroxidase (MnP) from Bacillus pumilus and Paenibacillus sp. was studied under absence and presence of the inducers indulin AT, guayacol, veratryl alcohol, lignosulfonic acid and lignosulfonic acid desulfonated. Indulin AT increased the activity of B. pumilus MnP up to 31.66 U/L after 8 h, but no improve was observed for Paenibacillus sp., which reached maximum activity (12.22 U/L) after 20 h. Both MnPs produced by these microorganisms were purified in phenyl sepharose resin and the proteins from crude extracts were eluted in two fractions. However, only the first fraction of each extract exhibited MnP activities. Tests in different pH and temperature values, from pH 5.0 to pH 10.0 and 30 ºC to 60 ºC, respectively, were carried...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus pumilus; Paenibacillus sp.; Manganese peroxidase; Purification; Characterization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400012
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Rizobactérias associadas a materiais orgânicos no controle de nematoides das galhas em tomateiro Horticultura Brasileira
Silva,Fabíola J; Ribeiro,Regina CF; Xavier,Adelica A; Santos Neto,José A; Souza,Maione A; Dias-Arieira,Cláudia R.
RESUMO: Agentes de controle biológico associados com matéria orgânica é uma estratégia que pode potencializar o controle dos nematoides-das-galhas. Objetivou-se neste trabalho avaliar o efeito de materiais orgânicos e rizobactérias sobre Meloidogyne javanica em tomateiro. Utilizou-se o delineamento em blocos casualizados, em esquema fatorial 4x2+1, sendo quatro materiais orgânicos (turfa, biofertilizante, esterco bovino e composto orgânico de casca de pequi), dois isolados de rizobactérias (Bacillus pumilus -1 e B. pumilus - 76) e adubação mineral (tratamento adicional), com oito repetições. Mudas de tomateiro foram transplantadas para vasos contendo solo arenoso, ao qual incorporaram-se os diferentes materiais orgânicos. As suspensões das rizobactérias...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; Meloidogyne javanica; Controle biológico; Bacillus pumilus; Matéria orgânica..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362016000100059
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Structural characterization of the bglH gene encoding a beta-glucosidase-like enzyme in an endophytic Bacillus pumilus strain Genet. Mol. Biol.
Bogas,Andréa C.; Watanabe,Maria Angelica E.; Barbosa,Aneli; Vilas-Boas,Laurival A.; Bonatto,Ana C.; Dekker,Robert; Souza,Emanuel M.; Fungaro,Maria Helena P..
A beta-glucosidase-like enzyme-encoding gene (bglH) of an endophytic Bacillus pumilus strain (CL16) was cloned using a shotgun genomic library constructed in Escherichia coli. The nucleotide sequence of the entire cloned fragment (2484 bp) was determined and characterized. An incomplete open reading frame (ORF) of 534 bp (ORF1) designated bglP and a complete ORF of 1419 bp (ORF2) designated bglH, located in the fragment, are organized in an operon. The protein deduced from 1419 bp (ORF2) had 472 amino acid residues without a characteristic signal peptide sequence, suggesting that the enzyme is localized in the cytoplasm. The amino acid sequence deduced from bglH gene had high similarity with beta-glucosidases from the glycosyl hydrolase family 1....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus pumilus; BglH; Glucosidase; Glycosyl hydrolase 1; PTS.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100018
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Use of Bacillus pumilus CBMAI 0008 and Paenibacillus sp. CBMAI 868 for colour removal from paper mill effluent BJM
Oliveira,Patrícia Lopes de; Duarte,Marta Cristina Teixeira; Ponezi,Alexandre Nunes; Durrant,Lúcia Regina.
Bacillus pumilus and Paenibacillus sp. were applied on the paper mill effluent to investigate the colour remotion. Inocula were individually applied in effluent at pH 7.0, 9.0 and 11.0. The real colour and COD remotion after 48h at pH 9.0 were, respectively, 41.87% and 22.08% for B. pumilus treatment and 42.30% and 22.89% for Paenibacillus sp. Gel permeation chromatography was used to verify the molar masses of compounds in the non-treated and treated effluent, showing a decrease in the compounds responsible for the paper mill effluent colour.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus pumilus; Paenibacillus sp.; Colour removal; Paper mill effluent.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200027
Registros recuperados: 16
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