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| Registros recuperados: 24 | |
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| Liu,Xiao Lei; Liu,Su Lin; Liu,Min; Kong,Bi He; Liu,Lei; Li,Yan Hong. |
| Investigating the endophytic bacterial community in special moss species is fundamental to understanding the microbial-plant interactions and discovering the bacteria with stresses tolerance. Thus, the community structure of endophytic bacteria in the xerophilous moss Grimmia montana were estimated using a 16S rDNA library and traditional cultivation methods. In total, 212 sequences derived from the 16S rDNA library were used to assess the bacterial diversity. Sequence alignment showed that the endophytes were assigned to 54 genera in 4 phyla (Proteobacteria, Firmicutes, Actinobacteria and Cytophaga/Flexibacter/Bacteroids). Of them, the dominant phyla were Proteobacteria (45.9%) and Firmicutes (27.6%), the most abundant genera included Acinetobacter,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bacterial diversity; Endophytes; Moss; Molecular method; Cultivated isolates. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100022 |
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| Gatesoupe, Joel; Coves, Denis; Ortega, Aurelio; Papandroulakis, Nikos; Vadstein, Olav; De La Gandara, Fernando. |
| During the first 2 years of larval rearing trials with Atlantic bluefin tuna, survival was a challenging issue. As bacterial colonization of the gut has been shown to play a key role for other species, we studied the profiles of the microbiota associated with individual larvae at different stages in three distant hatcheries. The Bacterial Community Profile (BCP) was quantified based on PCR-DGGE analyses of partial amplicons from 16S rDNA. Considerable individual variability in BCP was observed before onset of feeding, and the BCP did not show regular fluctuation during ontogenesis. Microalgae were added to the rearing tanks in two of the three hatcheries, but it was not possible to distinguish the effect of location from the effect of algal addition on... |
| Tipo: Text |
Palavras-chave: Larval rearing; Bacterial diversity; DGGE; Thunnus thynnus. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00075/18648/16197.pdf |
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| Pizzetti, Ilaria; Gobet, Angelique; Fuchs, Bernhard M.; Amann, Rudolf; Fazi, Stefano. |
| The phylum Planctomycetes is involved in important processes, such as the mineralization of algal biomass and the removal of nitrogen. Using a combination of 16S rRNA sequence analysis and in situ hybridization, we analyzed the diversity and dynamics of Planctomycetes in a shallow meso-eutrophic lake, Lago di Paola, Italy. Planctomycetes detected by the probe PLA46 accounted for 1 to 5% of prokaryotic picoplankton. Abundances were higher in the coastal lake than in the adjacent marine waters. In the surface waters of the lake, the numbers of Planctomycetes fluctuated greatly, reaching a maximum of 1.5 x 10(6) cells ml(-1) in July. The hypoxic bottom waters had less variable cell abundances. The Planctomycetes counts were positively correlated with... |
| Tipo: Text |
Palavras-chave: Coastal lake ecology; Planctomycetes; Bacterial diversity; FISH; CARD-FISH; 16S rRNA gene sequencing. |
| Ano: 2011 |
URL: https://archimer.ifremer.fr/doc/00488/59948/63196.pdf |
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| Cenciani,Karina; Lambais,Marcio Rodrigues; Cerri,Carlos Clemente; Azevedo,Lucas Carvalho Basílio de; Feigl,Brigitte Josefine. |
| It is well-known that Amazon tropical forest soils contain high microbial biodiversity. However, anthropogenic actions of slash and burn, mainly for pasture establishment, induce profound changes in the well-balanced biogeochemical cycles. After a few years the grass yield usually declines, the pasture is abandoned and is transformed into a secondary vegetation called "capoeira" or fallow. The aim of this study was to examine how the clearing of Amazon rainforest for pasture affects: (1) the diversity of the Bacteria domain evaluated by Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis (PCR-DGGE), (2) microbial biomass and some soil chemical properties (pH, moisture, P, K, Ca, Mg, Al, H + Al, and BS), and (3) the influence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Land use changes; PCR-DGGE; Bacterial diversity; Microbial biomass; Principal component analysis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000400015 |
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| Fernandes,Camila Cesário; Kishi,Luciano Takeshi; Lopes,Erica Mendes; Omori,Wellington Pine; Souza,Jackson Antonio Marcondes de; Alves,Lucia Maria Carareto; Lemos,Eliana Gertrudes de Macedo. |
| Abstract Human activities on the Earth's surface change the landscape of natural ecosystems. Mining practices are one of the most severe human activities, drastically altering the chemical, physical and biological properties of the soil environment. Bacterial communities in soil play an important role in the maintenance of ecological relationships. This work shows bacterial diversity, metabolic repertoire and physiological behavior in five ecosystems samples with different levels of impact. These ecosystems belong to a historical area in Iron Quadrangle, Minas Gerais, Brazil, which suffered mining activities until its total depletion without recovery since today. The results revealed Proteobacteria as the most predominant phylum followed by Acidobacteria,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Iron mine; Bacterial diversity; Functional diversity; Brazilian soils. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300489 |
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| Velázquez-Sepúlveda,I; Orozco-Mosqueda,MC; Prieto-Barajas,CM; Santoyo,G. |
| Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gammaproteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diversity; Rhizosphere; Wheat; 16S ribosomal genes. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100012 |
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| He,Suxu; Zhou,Zhigang; Banerjee,Goutam; Huang,Lu; Ray,Arun Kumar; Ringø,Einar. |
| ABSTRACT An investigation was conducted to identify the allochthonous microbiota (entire intestine) and the autochthonous microbiota in proximal intestine (PI) and distal intestine (DI) of four species of Indian air-breathing fish (climbing perch; Anabas testudineus, murrel; Channa punctatus, walking catfish; Clarias batrachus and stinging catfish; Heteropneustes fossilis) by PCR based denaturing gradient gel electrophoresis (DGGE). High similarities of the allochthonous microbiota were observed between climbing perch and murrel, walking catfish and stinging catfish, indicating similar food behavior. The autochthonous microbiota of PI and DI from climbing perch and murrel revealed more similarity, than the result obtained from walking catfish and stinging... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allochthonous and autochthonous gut microbiota; Air-breathing fish; Bacterial diversity; DGGE. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100350 |
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| Gobet, Angelique; Boeer, Simone I.; Huse, Susan M.; Van Beusekom, Justus E. E.; Quince, Christopher; Sogin, Mitchell L.; Boetius, Antje; Ramette, Alban. |
| Coastal sands filter and accumulate organic and inorganic materials from the terrestrial and marine environment, and thus provide a high diversity of microbial niches. Sands of temperate climate zones represent a temporally and spatially highly dynamic marine environment characterized by strong physical mixing and seasonal variation. Yet little is known about the temporal fluctuations of resident and rare members of bacterial communities in this environment. By combining community fingerprinting via pyrosequencing of ribosomal genes with the characterization of multiple environmental parameters, we disentangled the effects of seasonality, environmental heterogeneity, sediment depth and biogeochemical gradients on the fluctuations of bacterial communities... |
| Tipo: Text |
Palavras-chave: 454 pyrosequencing; Coastal seas; Bacterial diversity; Multivariate analysis; Rare biosphere. |
| Ano: 2012 |
URL: https://archimer.ifremer.fr/doc/00488/59947/63192.pdf |
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| ARAÚJO,FÁBIO V. DE; NETTO,MARCELLE C.M.; AZEVEDO,GUSTAVO P.; JAYME,MARCELLY M.A.; NUNES-CARVALHO,MONICA C.; SILVA,MARIANA M.; CARMO,FLÁVIA L. DO. |
| ABSTRACT Marine sponges has been a large reservoir of microbial diversity, with the presence of many species specific populations as well as producing biologically active compounds, which has attracted great biotechnological interest. In order to verify the influence of the environment in the composition of the bacterial community present in marine sponges and biotechnological potential of bacteria isolated from these organisms, three species of sponges and the waters surrounding them were collected in different beaches of Rio de Janeiro, Brazil. The profile of the bacterial community present in sponges and water was obtained by PCR-DGGE technique and the biotechnological potential of the strains isolated by producing amylase, cellulase, protease and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diversity; Biosurfactants; Enzymes; Marine sponges; PCR-DGGE.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602785 |
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| Rachedi,Kounouz; Zermane,Ferial; Tir,Radja; Ayache,Fatima; Duran,Robert; Lauga,Béatrice; Karama,Solange; Simon,Maryse; Boulahrouf,Abderrahmane. |
| ABSTRACT Repeated application of pesticides disturbs microbial communities and cause dysfunctions on soil biological processes. Granstar® 75 DF is one of the most used sulfonylurea herbicides on cereal crops; it contains 75% of tribenuron-methyl. Assessing the changes on soil microbiota, particularly on the most abundant bacterial groups, will be a useful approach to determine the impact of Granstar® herbicide. For this purpose, we analyzed Actinobacteria, which are known for their diversity, abundance, and aptitude to resist to xenobiotic substances. Using a selective medium for Actinobacteria, 42 strains were isolated from both untreated and Granstar® treated soils. The number of isolates recovered from the treated agricultural soil was fewer than that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diversity; Actinobacteria; Granstar® herbicide; Resistance; 16S rRNA genes. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100079 |
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| EVANS, F. F.; ROSADO, A. S.; SEBASTIÁN, G. V.; CASELLA, R.; MACHADO, P. L. O. de A.; HOLMSTRÖM, C.; KJELLEBERG, S.; ELSAS, J. D. van; SELDIN, L.. |
| The aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an... |
| Tipo: Artigo de periódico |
Palavras-chave: Atlantic forest; Bacterial diversity; Biostimulation; Crude oil; DGGE; 16S rRNA gene; Soil microcosms; Cambisols. |
| Ano: 2004 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135450 |
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| BABUJIA, L. C.; SILVA, A. P.; NOGUEIRA, M. A.; HUNGRIA, M.. |
| ABSTRACT: The no-tillage (NT) system of soil management is recognized as more sustainable than conventional tillage (CT), with an important role played by soil microorganisms. The objective of this study was to estimate differences in soil microbial diversity under NT and CT at different soil depths. For that, bacterial (16S rDNA) and fungal (18S rDNA) communities were evaluated by denaturing gradient gel electrophoresis (DGGE) in a 20-year field experiment established on an Oxisol in southern Brazil on which soybean has been grown in the summer and wheat in the winter. Soil samples were collected at the depths of 0-5, 5-10, 10-20 and 20-30 cm, and submitted to DGGE analyses. The results revealed lower similarity (28%) between bacterial communities in the... |
| Tipo: Separatas |
Palavras-chave: Bacterial diversity; Fungal diversity; Microbial.. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/996519 |
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| Vargas,Rafael S.; Bataiolli,Renata; da Costa,Pedro B.; Lisboa,Bruno; Passaglia,Luciane Maria P.; Beneduzi,Anelise; Vargas,Luciano K.. |
| The aim of this study was to evaluate the impact of different grazing pressures on the activity and diversity of soil bacteria. We performed a long-term experiment in Eldorado do Sul, southern Brazil, that assessed three levels of grazing pressure: high pressure (HP), with 4% herbage allowance (HA), moderate pressure (MP), with 12% HA, and low pressure (LP), with 16% HA. Two reference areas were also assessed, one of never-grazed native vegetation (NG) and another of regenerated vegetation after two years of grazing (RG). Soil samples were evaluated for microbial biomass and enzymatic (β-glucosidase, arylsulfatase and urease) activities. The structure of the bacterial community and the population of diazotrophic bacteria were evaluated by RFLP of the 16S... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diversity; Diazotrophic bacteria; Diversity; Grasslands; Soil microbial communities. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200205 |
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| Registros recuperados: 24 | |
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