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BMP-2 and titanium particles synergistically activate osteoclast formation BJMBR
Sun,S.X.; Guo,H.H.; Zhang,J.; Yu,B.; Sun,K.N.; Jin,Q.H..
A previous study showed that BMP-2 (bone morphogenetic protein-2) and wear debris can separately support osteoclast formation induced by the receptor activator of NF-κB ligand (RANKL). However, the effect of BMP-2 on wear debris-induced osteoclast formation is unclear. In this study, we show that neither titanium particles nor BMP-2 can induce osteoclast formation in RAW 264.7 mouse leukemic monocyte macrophage cells but that BMP-2 synergizes with titanium particles to enhance osteoclast formation in the presence of RANKL, and that at a low concentration, BMP-2 has an optimal effect to stimulate the size and number of multinuclear osteoclasts, expression of osteoclast genes, and resorption area. Our data also clarify that the effects caused by the increase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bone morphogenetic protein; Titanium; Osteoclasts; SMAD; RANKL.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600461
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DDS技術を活用した獣医再生治療 OAK
佐々木, 直樹.
Recently, regenerative medicine with autologous cells and tissues has been carried out for tissue regeneration. Basic research and clinical application based on tissue engineering have also been reported in joint cartilage regeneration. The physiological connection between the cartilages newly regenerated and natural ones is not always sufficient. Currently, to tackle the problem, the clinical application of DDS-based cartilage regeneration therapy has been tried. This paper introduces the research results of bone regeneration and articular cartilage regeneration in the veterinary medical field. c 2017, Japan Society of Drug Delivery System. All rights reserved.
Palavras-chave: Basic fibroblast factor; Bone morphogenetic protein; Platelet rich plasma; Transforming growth factor-β1; Β-tricalcium phosphate.
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4543
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Neuropeptide Y, substance P, and human bone morphogenetic protein 2 stimulate human osteoblast osteogenic activity by enhancing gap junction intercellular communication BJMBR
Ma,W.H.; Liu,Y.J.; Wang,W.; Zhang,Y.Z..
Bone homeostasis seems to be controlled by delicate and subtle “cross talk” between the nervous system and “osteo-neuromediators” that control bone remodeling. The purpose of this study was to evaluate the effect of interactions between neuropeptides and human bone morphogenetic protein 2 (hBMP2) on human osteoblasts. We also investigated the effects of neuropeptides and hBMP2 on gap junction intercellular communication (GJIC). Osteoblasts were treated with neuropeptide Y (NPY), substance P (SP), or hBMP2 at three concentrations. At various intervals after treatment, cell viability was measured by the MTT assay. In addition, cellular alkaline phosphatase (ALP) activity and osteocalcin were determined by colorimetric assay and radioimmunoassay,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neuropeptide; Bone morphogenetic protein; Osteoblast; Gap junction intercellular communication.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400299
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Role of bone morphogenetic proteins in follicle development. Infoteca-e
SOUZA, C. J. H.; MELO, E. O.; MORAES, J. C. F.; BAIRD, D. T..
Tipo: Parte de livro Palavras-chave: Bone morphogenetic protein.
Ano: 2007 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/190827
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