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Registros recuperados: 22
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Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli. Repositório Alice
SANTOS, M. R. A. dos; PAZ, E. S..
In vitro cell suspension cultivation systems have been largely reported as safe and standardized methods of production of secondary metabolites with medicinal and agricultural interest. Capsicum frutescensis one of the most widely grown vegetable in the world and its biological activities have been demonstrated against insects, fungi, bacteria and other groups of organisms. The objective of this study was to establish a protocol for dedifferentiation of leaf cells of the cultivar C. frutescens cv. Stromboli and to determine the growth pattern of the calluses with a focus on the deceleration phase, when the callus cells must be subcultured into a liquid medium in order to establish cell suspension cultivations aiming at the production of secondary...
Tipo: Artigo de periódico Palavras-chave: Capsicum frutescens cv Stromboli; Célula foliar; Metabólicos secundários; Protocolo de diferenciação celular; Leaf cells; Callogenesis; Growth curves; Deceleration phase; Fase de desaceleração; Embrapa Rondônia; Porto Velho (RO); Rondônia; Amazônia Ocidental; Western Amazon; Cultura In Vitro; Calogênese; Curva de Crescimento; In vitro culture; Callus formation; Secondary metabolites; Cell dedifferentiation.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127111
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Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama. Repositório Alice
SANTOS, M. R. A. dos; AGUIAR, C. B.; MENDES, A. M..
Chili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus...
Tipo: Separatas Palavras-chave: Callogenesis; Growlh curve.; Secondary metabolites..
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080950
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Dedifferentiation of leaf cells and growth of friable calluses of Capsicum annuum CV. ALL. BIG. Repositório Alice
SANTOS, M. R. A. dos; SOUZA, C. A..
Tipo: Separatas Palavras-chave: Callogenesis; Growth curve.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1097134
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Dedifferentiation of Leaf Cells and Growth Pattern of Calluses of Capsicum annuumcv. Etna. Repositório Alice
SANTOS, M. R. A. dos; SOUZA, C. A. de.
Background:In vitrocell suspension cultivation systems have been largely reported assafe and standardized methods for production of secondary metabolites with medicinaland agricultural interest.Capsicum annuumis one of the most widely grown vegetablein the world and its biological activities have been demonstrated against insects, fungi,bacteria and other groups of organisms. The determination of procedures for thededifferentiation of cells into callus cells and the subsequent study of the callus growthpattern are necessary for the establishment of cellsuspensions and also to subsidizestudies regarding the bioactivity of its secondarymetabolites. To date, no study hasdescribed the development of protocols for callus induction inC. annuumL. cv. Etna....
Tipo: Artigo de periódico Palavras-chave: Callogenesis; Growth curve; Metabolismo secundário; Calogêneses.; Curva de Crescimento; Pimenta.; Secondary metabolites..
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053310
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Early somatic embryogenesis in Heliconia chartacea Lane ex Barreiros cv. Sexy Pink ovary section explants BABT
Ulisses,Cláudia; Camara,Terezinha Rangel; Willadino,Lilia; Albuquerque,Cynthia Cavalcanti de; Brito,Júlio Zoé de.
The present work evaluated the development of embryogenic callus from transversal ovary sections. The experiments were carried out under two experimental regimes using combinations of IAA (0; 5.71; 8.56; 11.42; 14.27μM) and 2,4-D (0; 13.57; 18.10; 22.62μM) or combinations of 2,4-D with BA (0; 4.43; 6.65; 8.87; 11.09μM). Assessments were made of anatomical aspects of the callus and for the presence of embryogenic structures using cytochemical and histological analyses and stereomicroscopic and scanning electronic microscopic observations. Treatments with 2,4-D and IAA produced friable calluses demonstrating cellular acquisition of morphogenetic competence as well as the formation of pro-embryogenic sectors. The expression of embryogenic program could be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Callogenesis; Growth regulator; Histology; Scanning electron microscopy.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100002
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Effect of 2,4-D and BA on the establishment of cell suspension from nodes and internodes of Capsicum annuum cv. Etna. Repositório Alice
SANTOS, M. R. A. dos; SOUZA, C. A. de.
Tipo: Artigo de periódico Palavras-chave: Callogenesis; Growth curve.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116265
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Effect of 2,4-d on callus induction in leaf explants of peach palm (bactris gasipaes h.b.k.). Repositório Alice
SANTOS, M. R. A. dos; PAZ, E. S..
The objective of this research was to determine the effect of dichlorophenoxyacetic acid (2,4-D) callus in peach friable calluses in leaf explants of Bactris gasipaes H.B.K., aiming for further induction of somatic embryos. The explants were inoculated in MS medium supplemented with 30.0 g.L-1 sucrose, 8.0 g.L-1 agar and 2,4-D (0.0, 0.31, 0.62, 1.25, 2.5, 5.0, 10.0, and 20.0 mg.L-1). The cultures were kept in a growth room at 24±2°C under light conditions (50 µmol.m-2.s-1, photoperiod of 16 hours), and also under dark conditions, in factorial arrangement: 2 (light and dark conditions) x 8 (2,4-D concentrations), totaling 16 treatments. On the 14th day of cultivation, occurrences of oxidation and necrosis of the explants were observed and, on the 35th day,...
Tipo: Artigo de periódico Palavras-chave: Peach palm; Callogenesis; Dichlorophenoxyacetic acid; Pessegueiro; Ácido diclorofenoxiacético.; Calogênese.; Arecaceae..
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1057485
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Growth characteristics and dynamics of protein synthesis in callus cultures from Glycine wightii (Wight & Arn.) Verdc. Ciência e Agrotecnologia
Silva,André Luis Coelho da; Caruso,Cecília Sulzbacher; Moreira,Renato de Azevedo; Horta,Ana Cecília Góes.
Cotyledon explants were first cultured on MS medium supplemented with 4.52 M 2,4-D and 0.46 mM kinetin. The development of the calli was followed (0, 4, 8, 12, 16, 20, 24, 28 and 32 days after) and the growth curve was determined, based in fresh and dry weight. The growth curve presented sigmoidal form with four distinct phases. The highest growth percentage was observed at the exponential phase and the lowest at the stationary phase. These results indicated that cotyledon callus subculture should be performed 20 days after inoculation. The calli obtained after a period of 28 days were freeze dried, macerated and submitted to extraction with buffers of different pH values (2.6; 4.0; 6.0; 8.0 and 10.0) and the proteins in the extracts were determined by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine; Leguminosae; Callogenesis; SDS-PAGE.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000600009
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Growth pattern of friable callus from P. Carniconnectivum Leaf explants. Repositório Alice
SANTOS, M. R. A. dos; SOUZA, C. A. de; GUIMARÃES, M. de C. M.; SMOZINSKI, C. V.; MAGALHÃES, G. M. de O.; NOGUEIRA, W. O.; PAZ, E. S..
The objective of this study was the in vitro induction of friable callus in leaf explants of P. carniconnectivum, by determining the appropriate hormonal combination to produce high callus cell proliferation, and the identification of the growth pattern of the calluses focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension culture.
Tipo: Artigo de periódico Palavras-chave: Callus growth pattern; Callogenesis; Piperaceae; Growth regulators.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1027742
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Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño. Repositório Alice
SANTOS, M. R. A. dos; SOUZA, C. A. de; PAZ, E. S..
The genus Capsicum belongs to the Solanaceae botanical family and is notable for the production of secondary metabolites of medicinal and economical importance. In vitro methods have been successfully utilized for the large scale production of plant secondary metabolites. The objective of this study was to establish a protocol for callus induction from leaves, nodal and internodal segments of the cultivar C. annuumvar. annuumcv. Iberaba Jalapeño, and to determine the growth pattern of the calluses, aiming at the identification of the deceleration phase, when the callus cells must be subcultured for the establishment of cell suspensions and the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in...
Tipo: Separatas Palavras-chave: Callogenesis; Growth curve.; Secondary metabolites..
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080890
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Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño Rev. Ciênc. Agron.
Santos,Mauricio Reginaldo Alves dos; Souza,Carolina Augusto de; Paz,Eloísa Santana.
ABSTRACT The genus Capsicum belongs to the Solanaceae botanical family and is notable for the production of secondary metabolites of medicinal and economical importance. In vitro methods have been successfully utilized for the large scale production of plant secondary metabolites. The objective of this study was to establish a protocol for callus induction from leaves, nodal and internodal segments of the cultivar C. annuum var. annuum cv. Iberaba Jalapeño, and to determine the growth pattern of the calluses, aiming at the identification of the deceleration phase, when the callus cells must be subcultured for the establishment of cell suspensions and the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Callogenesis; Growth curve; Secondary metabolites.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000300523
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In vitro cultivation of callus cells from nodes and internodes of Capsicum annuum cv. All Big. Repositório Alice
SANTOS, M. R. A. dos; SOUZA, C. A. de.
Tipo: Artigo de periódico Palavras-chave: Callogenesis; Growth curve.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116268
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In vitro induction of callus from cotyledon and hypocotyl explants of Glycine wightii (Wight & Arn.) Verdc. Ciência e Agrotecnologia
Silva,André Luis Coelho da; Caruso,Cecília Sulzbacher; Moreira,Renato de Azevedo; Horta,Ana Cecília Góes.
With the objective to promote in vitro callus induction, cotyledon and hypocotyl segments of "perennial soybean" (Glycine wightii (Wight & Arn.) Verdc.) were inoculated in basal medium MS supplemented with sucrose (1.5 e 3%) and 0.8% agar and different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-furfurylaminopurine (kinetin). The explants were maintained in a dark growth room at 28ºC. The best callus induction was observed in explants (cotyledon and hypocotyl) maintained in medium containing the combination of 2,4-D (1 mg.L-1), kinetin (0.1 mg.L-1) and 3% sucrose. To promote callus subculture, the MS medium was supplemented with different combinations of 2,4-D (0.5 to 4.0 mg.L-1), with or without kinetin (0.1 mg.L-1) and sucrose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine wightii; Leguminosae; Callogenesis; Tissue culture.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000600011
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In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae) Acta Botanica
Balbuena,Tiago Santana; Santa-Catarina,Claudete; Silveira,Vanildo; Kato,Massuo Jorge; Floh,Eny Iochevet Segal.
Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l-1) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l-1 2,4-D and 2 mg.l-1 BA supplemented medium, and in the absence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Callogenesis; Cell suspension culture; Morphogenesis; Pariparoba; Plant growth regulators.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062009000100029
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In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium Agronomy
Brondani, Gilvano Ebling; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Araujo, Marla Alessandra; Universidade Federal de Lavras; Alcântara, Berenice Kussumoto; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Carvalho, Janice Guedes; Universidade Federal de Lavras; Gonçalves, Antonio Natal; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Almeida, Marcilio de; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP).
The in vitro organogenesis of woody species plays an essential role in the improvement of forest products by providing saplings with high commercial value. Furthermore, mineral nutrition plays an important role in the induction of organogenic responses. The objective of this study was to evaluate the effects of boron and calcium in the organogenesis of nodal segments from seedlings of Eucalyptus grandis growing under in vitro conditions. The concentration of boron and calcium in MS medium was modified to induce organogenic responses in 45-day-old nodal segments used as explants. After 60 days, the fresh weight, dry weight, ratio of fresh and dry weight, relative water content and relative matter content accumulated by the explants were evaluated. The...
Tipo: Pesquisa com fatores controlados Palavras-chave: 5.01.03.04-0 bud induction; Callogenesis; Tissue culture; Mineral nutrition 5.01.03.04-0 bud induction; Callogenesis; Tissue culture; Mineral nutrition.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15143
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Indução de calo em explante de pimenta-do-reino (Piper nigrum L.). Repositório Alice
NUNES, H. da C. B.; MOTA, M. G. da C..
Tipo: Parte de livro Palavras-chave: Pimenta-do-reino; Resistência a doença; Explantes; Callogenesis; Calo; Cultura In Vitro; Explante; Fusarium Solani; Piper Nigrum; Disease resistance; In vitro culture.
Ano: 1995 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/394572
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Indução de calos em explantes foliares de Cissus verticillata (l.) Nicolson & C. E. Jarvis. Repositório Alice
SANTOS, M. R. A. dos; ROCHA, J. F. da; PAZ, E. S.; SMOZINSKI, C. V.; NOGUEIRA, W. de O.; GUIMARÃES, M. de C. M..
O objetivo desta pesquisa foi desenvolver um protocolo para indução de calos em explantes foliares de C. verticillata, visando subsidiar o futuro estabelecimento de sistemas de células em suspensão para a produção de metabólitos secundários in vitro. The objective of this research was to develop a protocol for callus induction in leaf explants of C. verticillata, aiming to provide support for further establishment of cell suspension systems for in vitro production of secondary metabolites.
Tipo: Artigo de periódico Palavras-chave: Callogenesis; Medicinal plant; Metabólitos secundários.; Calogênese; Planta Medicinal.; Secondary metabolites..
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1005600
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Induction and growth pattern of callus from Piper permucronatum leaves. Repositório Alice
SANTOS, M. R. A. dos; GUIMARÃES, M. C. M.; PAZ, E. S.; MAGALHÃES, G. M. O.; SOUZA, C. A.; SMOZINSKI, C. V.; NOGUEIRA, W. O..
Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf?s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to...
Tipo: Artigo de periódico Palavras-chave: Callogenesis; Reguladores de crescimento.; Calogênese; Piperaceae.; Growth regulators..
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1045154
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Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit BABT
Carvalho,Milene Alves de Figueiredo; Paiva,Renato; Stein,Vanessa Cristina; Herrera,Raírys Cravo; Porto,Jorge Marcelo Padovani; Vargas,Daiane Peixoto; Alves,Eduardo.
This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passifloraceae; Callogenesis; Electromicroscopy; Optic microscope; Tissue culture.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851
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Influência de auxinas e tipos de explantes na indução de calos friáveis em Piper hispidinervum C. DC. Repositório Alice
COSTA, F. H. da S.; LOUREIRO, T. da S.; PEREIRA, J. E. S..
Tipo: Separatas Palavras-chave: Callogenesis; Cells culture; Phytoregulators; Pimenta.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/190633
Registros recuperados: 22
Primeira ... 12 ... Última
 

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