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Registros recuperados: 21
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A genomewide association mapping study using ultrasound-scanned information identifies potential genomic regions and candidate genes affecting carcass traits in Nellore cattle. Repositório Alice
SANTANA, M. H. A.; GOMES, R. da C.; BONIN, M. N..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bos indicus; Candidate genes; Fat thickness; Genomewide association studies; Single nucleotide polymorphisms; Ultrasonography.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1030961
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Análises da expressão gênica por PCR quantitativo em tempo real (QPCR) de genes candidatos para a tolerância à seca em cafeeiro. Repositório Alice
VINECKY, F.; ALVES, G. S. C.; VIEIRA, N. G.; FREIRE, L. P.; HEIMBECK, I. G. R.; FERRÃO, R. G.; FONSECA, A. F. A. da; FERRAO, M. A. G.; SILVA, V. A.; MARRACCINI, P.; ANDRADE, A. C..
A seca é uma das principais limitações climáticas à produção do cafeeiro. A importância dessa limitação deve aumentar, em função das mudanças reconhecidas no clima global e, também, porque a cafeicultura vem sendo expandida para regiões marginais onde a baixa pluviosidade e temperaturas desfavoráveis se constituem grandes limitações à produção do café. A seca induz diversas respostas fisiológicas e moleculares nas plantas, incluindo alterações da expressão gênica, visando atingir ajuste osmótico, a indução de reparadores de sistemas moleculares e a expressão de diversas proteínas protetoras. Existem diversas formas de se avaliar a expressão gênica em plantas submetidas ao estresse hídrico. Uma técnica amplamente utilizada nos últimos anos, para a análise...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Coffea canephora; Expressão gênica; Gene candidato; Seca; Coffea canephora; Candidate genes; Drought; Gene expression.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/903864
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Assessment of the association between SMAD1 and HHIP gene variation and non-syndromic cleft-lip palate in Chilean case-parent trios Genet. Mol. Biol.
Suazo,José; Santos,José Luis; Jara,Lilian; Blanco,Rafael.
Nonsyndromic cleft lip/palate (NSCLP) is a congenital malformation with features of a complex genetic trait. Several studies have reported positive association and linkage between NSCLP and microsatellite markers in the 4q28-4q33 region particularly with the D4S192 (4q31) marker. We hypothesized that the candidate genes SMAD1 and HHIP (4q31) could be involved in the etiology of NSCLP based on previous positive linkage results and their important role in maxillofacial development. We evaluated the possible association between microsatellite markers located at less than 1 cM from these genes and NSCLP using a sample of 58 Chilean case-parent trios. Microsatellite markers were analyzed using the polymerase chain reaction (PCR) with fluorescent labeled...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidate genes; Case-parents trio design; Linkage disequilibrium; Nonsyndromic cleft lip/palate.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400006
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Association between G316A growth hormone polymorphism and economic traits in pigs Genet. Mol. Biol.
Faria,Danielle Assis de; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Pires,Aldrin Vieira; Paiva,Samuel Rezende; Sollero,Bruna Pena; Wenceslau,Amauri Arias.
The association between G316A growth hormone polymorphism and quantitative traits was investigated in an F2 population of pigs. Association analyses were performed using a statistical model that included genotype, sex, batch and sex by genotype interaction as fixed effects and sire as random effect. The polymorphism was associated with the number of right teats (p = 0.03), heart weight (p = 0.04), lung weight (p = 0.05), carcass length determined by the Brazilian carcass classification method (p = 0.04), picnic shoulder weight (p = 0.07), jowl weight (p = 0.01), pH 24 h after slaughtering (p = 0.03) and drip loss (p = 0.01). Interaction between genotype and sex was observed for six performance traits. The additive effect was significant (p < 0.10) for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidate genes; Divergent cross; Molecular marker; Sex x genotype interaction; Swine.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400010
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Can survival of European flat oysters following experimental infection with Bonamia ostreae be predicted using QTLs? ArchiMer
Harrang, Estelle; Heurtebise, Serge; Faury, Nicole; Robert, Maeva; Arzul, Isabelle; Lapegue, Sylvie.
The present study identifies quantitative trait loci (QTLs) in response to an experimental infection with the parasite responsible for bonamiosis, Bonamia ostreae, in two segregating families of the European flat oyster, Ostrea edulis. We first constructed a genetic-linkage map for each studied family and improved the existing genetic-linkage map for the European flat oyster with a set of SNP markers. This latter map now combines the best accuracy and the best estimate of the genome coverage available for an oyster species. Secondly, by comparing the QTLs detected in this study with those previously published for O. edulis in similar experimental conditions, we identified several potential QTLs that were identical between the different families, and also...
Tipo: Text Palavras-chave: Ostrea edulis; Single Nucleotide Polymorphism; Disease resistance; Genetic and QTL mapping; Candidate genes.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00271/38244/36429.pdf
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Candidate mutations used to aid the prediction of genetic merit for female reproductive traits in tropical beef cattle R. Bras. Zootec.
Fortes,Marina Rufino Salinas; Enculescu,Charmaine; Porto Neto,Laercio R.; Lehnert,Sigrid A.; McCulloch,Russell; Hayes,Ben.
ABSTRACT In this study, we aimed to provide a wet laboratory validation for a set of single nucleotide polymorphisms (SNP), which had been identified as candidate functional variants in silico. Genotyping for candidate SNP was performed in Brahman and Tropical Composite cattle. After quality control, 29 SNP were first investigated individually for their association with female reproductive traits and then used as a panel for genomic predictions. The reproductive traits studied were age at first corpus luteum (AGECL; days), post-partum anoestrus interval (PPAI; days), and a binary trait that described if the cow had ovulated before weaning the first calf or not (PW, 0-1). Single nucleotide polymorphisms in six genes (FOXA2, TRAF4, IRF2, IRF1, BPTF, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anoestrus; BayesR; Candidate genes; Genomic prediction; Heifer puberty.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100412
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Differentially expressed genes in drought stressed sugarcane Repositório Alice
DIAS, B. B. A.; SOUTO, B. de M.; LEAO, A. P.; LAGO, B. N.; MOLINARI, H. B. C..
2011
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Drought; Sugarcane; Differentially expressed sequences; Candidate genes; Brachypodium distachyon.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/908736
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Expression of VINV1, SCB1 and SCS1 genes in the seed coats of contrasting soybean seeds1 Rev. Ciênc. Agron.
Bahry,Carlos André; Zimmer,Paulo Dejalma.
The seed coat is vital for the proper development of soybean seeds and also, after their physiological maturity, for providing protection against various biotic and abiotic factors. The aim of this work was to evaluate the relative expression of three candidate genes, VINV1, SCB1 and SCS1, which are possibly involved in seed quality, in the contrasting seed coats of four soybean genotypes. Two yellow seed coat genotypes, BMX Potência RR and CD 202, and two black seed coat genotypes, TP and IAC, were used. Relative gene expression was evaluated by the qPCR technique for seven phases of seed coat development in the four genotypes, at 25, 30, 35, 40, 45, 50 and 55 days after anthesis. The design was completely randomised, with three replications. Data were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soybean; Coats; Candidate genes.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902015000100155
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Gene expression analysis reveals important pathways for drought response in leaves and roots of a wheat cultivar adapted to rainfed cropping in the Cerrado Biome. Repositório Alice
POERSCH-BORTOLON, L. B.; PEREIRA, J. F.; NHANI JUNIOR, A.; SOTO GONZÁLES, H. H.; TORRES, G. A. M.; CONSOLI, L.; ARENHART, R. A.; BODANESE-ZANETTINI, M. H.; MARGIS-PINHEIRO, M..
2016
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Triticum aestivum; Water deficit; 454 sequencing; Candidate genes; RT-qPCR; Trigo; Déficit hídrico; Sequenciamento de nova geração; Genes candidatos; PCR quantitativo; Tolerância à seca.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1057486
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Gene expression analysis reveals important pathways for drought response in leaves and roots of a wheat cultivar adapted to rainfed cropping in the Cerrado biome Genet. Mol. Biol.
Poersch-Bortolon,Liane Balvedi; Pereira,Jorge Fernando; Nhani Junior,Antonio; Gonzáles,Hebert Hernán Soto; Torres,Gisele Abigail Montan; Consoli,Luciano; Arenhart,Rafael Augusto; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia.
Abstract Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 454 sequencing; Candidate genes; RT-qPCR; Triticum aestivum; Water deficit.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400629
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Genetics of coffee quality Braz. J. Plant Physiol.
Leroy,Thierry; Ribeyre,Fabienne; Bertrand,Benóit; Charmetant,Pierre; Dufour,Magali; Montagnon,Christophe; Marraccini,Pierre; Pot,David.
Coffee quality, in the present context of overproduction worldwide, has to be considered as a main selection criterion for coffee improvement. After a definition of quality, and an overview of the non genetic factors affecting its variation, this review focuses on the genetic factors involved in the control of coffee quality variation. Regarding the complexity of this trait, the different types of quality are first presented. Then, the great variation within and between coffee species is underlined, mainly for biochemical compounds related to quality (caffeine, sugars, chlorogenic acids, lipids). The ways for breeding quality traits for cultivated species, Coffea arabica and Coffea canephora are discussed, with specific challenges for each species. For C....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea spp.; Biochemical compounds; Candidate genes; ESTs; Genetic breeding; Marker-assisted selection; Quality.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100016
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Identification of the transcriptionally active cytochrome P450 repertoire in Coffea arabica. Repositório Alice
IVAMOTO, S. T.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P..
Cytochrome P450s (P450s) comprise a gene superfamily encoding enzymes that are involved in diverse plant metabolic pathways that produce primary and secondary metabolites such as phenylpropanoids, terpenoids, nitrogen-containing compounds, and plant hormones. They comprise one of the most diverse gene families in plant evolution. Although there are many studies that aim to characterize P450s in plants, there is no report on the characterization of this superfamily in Coffea arabica, where they might be related to plant tolerance to biotic and abiotic stresses, as well as aroma-related compounds. In this study, we report the characterization and annotation of 87 putative P450s from C. arabica obtained from the Brazilian Coffee Genome Project and describe...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Expressed sequence tags (ESTs); Candidate genes; Coffea arábica; Cytochrome P-450; Transcriptome.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1039979
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Influence of temperament-related genes on live weight traits of Charolais cows R. Bras. Zootec.
Garza-Brenner,Estela; Sifuentes-Rincón,Ana María; Rodríguez-Almeida,Felipe Alonso; Randel,Ronald D.; Parra-Bracamonte,Gaspar Manuel; Arellano-Vera,Williams.
Abstract We investigated whether a panel of molecular markers previously associated with temperament has an effect on live growth traits. Phenotypic data from 412 Charolais cows categorized according to their age in adult and young cows were used to determine Pearson's correlations between birth (BW), weaning (WW), and yearling live (YW) weight and temperament traits (measured as exit velocity [EV] and temperament score [TS]). For association analysis, selective genotyping of a group of 80 cows identified as the most docile and temperamental were genotyped with a 151-SNP (single-nucleotide polymorphisms) panel of molecular markers previously associated with temperament. Significant Pearson's correlations between birth weight and weaning weight and the two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beef cattle; Behavior; Body weight; Candidate genes; SNP; Temperament.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982020000100401
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LINKAGE MAPPING OF CANDIDATE GENES FOR INDUCE RESISTANCE AND GROWTH PROMOTION BY Trichoderma koningiopsis (Th003) IN TOMATO Solanum lycopersicum Acta biol.Colomb.
SIMBAQUEBA,JAIME; COTES,ALBA MARINA; BARRERO,LUZ STELLA.
Induced systemic resistance (ISR) is a mechanism by which plants enhance defenses against any stress condition. ISR and growth promotion are enhanced when tomato (Solanum lycopersicum) is inoculated with several strains of Trichoderma ssp. This study aims to genetically map tomato candidate genes involved in ISR and growth promotion induced by the Colombian native isolate Trichoderma koningiopsis Th003. Forty-nine candidate genes previously identified on tomato plants treated with Th003 and T. hamatum T382 strains were evaluated for polymorphisms and 16 of them were integrated on the highly saturated genetic linkage map named &#8220;TOMATO EXPEN 2000&#8221;. The location of six unigenes was similar to the location of resistance gene analogs (RGAs),...
Tipo: Journal article Palavras-chave: Trichoderma koningiopsis; Solanum lycopersicum; Induced systemic resistance growth promotion; Candidate genes; Genetic linkage map.
Ano: 2011 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2011000200004
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Novel putative candidate genes associated with umbilical hernia in pigs. Repositório Alice
FERNANDES, L. T.; ONO, R. K.; IBELLI, A. M. G.; LAGOS, E. B.; MORES, M. A. Z.; CANTAO, M. E.; LORENZETTI, W. R.; PEIXOTO, J. de O.; PEDROSA, V. B.; LEDUR, M. C..
Abstract: Umbilical hernia is one of the most frequent anatomical defects in pigs in which abdominal contents protrude through the umbilical ring. This condition is considered to have a multifactorial basis in which environmental, infectious and genetic factors play a role. However, a better understanding about the genetic components involved in the umbilical hernia development has not yet been achieved. Although a few studies have mapped QTL for umbilical hernia, just a few candidate genes were reported. Therefore, the aim of this study was to identify genomic regions related to the development of umbilical hernias in pigs and search for potential candidate genes. A GWAS was performed with 92 cases and 233 control crossbred pigs. Five SNPs were associated...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Hérnia umbilical; Umbilical hernia; Candidate genes; Congenital defect; GWAS; Melhoramento Genético Animal; Suíno; Animal genetic resources; Swine.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1093239
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Polymorphism in Litopenaeus vannamei genes and cross-species amplification in other shrimp species PAB
Santos,Camilla Alves; Farias,Flavio; Teixeira,Ana Karina; Rocha,João Luís; Guerrelhas,Ana Carolina; Freitas,Patrícia Domingues de.
Abstract: The objective of this work was to assess polymorphisms within Litopenaeus vannamei candidate genes related to growth performance, and a possible association between weight genotypes and phenotypes in two shrimp closed lines. Three EST-SSR (serine-arginine, 60S ribosomal, and troponin) and one EST-SNP (crustacyanin) were selected and evaluated in L. vannamei and seven other marine shrimp species, besides two freshwater species. Genotypes had no effect on total body weight. Although no association was observed in the sampled population, these markers showed amplification for most of the evaluated species and, therefore, may be useful in further genetic studies on shrimp species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidate genes; Crustacyanin; Microsatellite; SNP; Weight-association; 60S ribosomal.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100121
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Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep PAB
Sousa Junior,Luis Paulo Batista; Meira,Ariana Nascimento; Azevedo,Hymerson Costa; Muniz,Evandro Nevez; Coutinho,Luiz Lehmann; Mourão,Gerson Barreto; Pedrosa,Victor Breno; Pinto,Luís Fernando Batista.
Abstract: The objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the Ovis aries genome. Genotype and allele frequencies were estimated, and the Hardy-Weinberg equilibrium was tested. Fragments containing 2,493 bp (MyoD1), 1,836 bp (MyoG), 2,813 bp (MyF5), 1,126 bp (MyF6), and 2,380 bp (MSTN) were obtained, and, in these sequences, 160 variants were identified. These polymorphisms were distributed as follows: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6), and 10 (MSTN). One hundred and four were novel polymorphisms, 45 in MyoD1, 2 in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovis aries; Candidate genes; Frequencies; Variants.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102810
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Prospecting candidate SNPs for backfat in Canchim beef cattle. Repositório Alice
VENERONI, G. B.; MEIRELLES, S. L.; GROSSI, D. A.; GASPARIN, G.; IBELLI, A. M. G.; TIZIOTO, P. C.; OLIVEIRA, H. N.; ALENCAR, M. M. de; REGITANO, L. C. de A..
Canchim is a composite cattle breed developed in Brazil for beef production. One of the breeding objectives is to increase fat deposition. QTLs for fat thickness and/or marbling have been reported on BTA4 and BTA14. The IGFBP3 and DDEF1 genes, mapped to BTA4 and BTA14, respectively, affect adipogenesis. We looked for SNPs in the IGFBP3 and DDEF1 genes that could be associated with backfat thickness in Canchim beef cattle. For SNP identification, sires with the highest accuracy were ranked according to expected breeding value for fat thickness; the 12 extremes (six sires with the highest and six with the lowest expected breeding value for the trait) were chosen. Six regions of the IGFBP3 and 14 regions of the DDEF1 were sequenced using the Sanger method....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Fat deposition; Candidate genes; SNP identification.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/864190
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Prospection for genes expressed during wheat - Magnaporthe oryzae interaction. Repositório Alice
GONZÁLES, H.; TORRES, G. A. M.; CONSOLI, L.; NHANI JUNIOR, A.; PEREIRA, J. F.; WEBBER, N. F..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Trigo; Doença; Brusone; Resistência genética; Seleção genética; Arista; Wheat blast; Genetic resistance; Candidate genes; Spikes.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1047903
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Relative expression of CYP and Arabinogalactan genes in soybean seed coats Rev. Ciênc. Agron.
Bahry,Carlos André; Venske,Eduardo; Perboni,Anelise Tessari; Zimmer,Paulo Dejalma.
ABSTRACT The aim of this study was to evaluate the relative expression of two candidate genes, CYP (cyclophilin) and Arabinogalactan, possibly involved with seed quality, in contrasting soybean seed coats. The following genotypes were used: BMX Potência RR and CD 202, both with yellow coats, and TP and IAC, with black coats. The relative expression of the genes was evaluated by qPCR in seven development phases of the coats of the four genotypes at 25; 30; 35; 40; 45; 50 and 55 days after anthesis. The design was completely randomized, with three replicates. The data were submitted to analysis of variance and the means were compared via the Scott Knott test, at 5% probability. The CYP gene showed greater expression in the intermediate stages of development...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Contrasting genotypes; Candidate genes; Cyclophilin; QPCR.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000100078
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