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| Registros recuperados: 12 | |
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| Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Campos,Magnólia A.; Teixeira,Juliana E.C.; Targon,Maria Luísa P.N.; Carlos,Eduardo F.; Ravasi,Juliano F.; Fischer,Carlos N.; Machado,Marcos A.. |
| The Citrus ESTs Sequencing Project (CitEST) conducted at Centro APTA Citros Sylvio Moreira/IAC has identified and catalogued ESTs representing a set of citrus genes expressed under relevant stress responses, including diseases such as citrus variegated chlorosis (CVC), caused by Xylella fastidiosa. All sweet orange (Citrus sinensis L. Osb.) varieties are susceptible to X. fastidiosa. On the other hand, mandarins (C. reticulata Blanco) are considered tolerant or resistant to the disease, although the bacterium can be sporadically detected within the trees, but no disease symptoms or economic losses are observed. To study their genetic responses to the presence of X. fastidiosa, we have compared EST libraries of leaf tissue of sweet orange Pêra IAC (highly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ESTs; Citrus variegated chlorosis; Biotic stress; CitEST; Phytopathogen. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500024 |
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| Lacava,Paulo Teixeira; Silva-Stenico,Maria Estela; Araújo,Welington Luiz; Simionato,Ana Valéria Colnaghi; Carrilho,Emanuel; Tsai,Siu Mui; Azevedo,João Lúcio. |
| The objective of this work was to study the production of siderophores by endophytic bacteria Methylobacterium spp., which occupy the same ecological niche as Xylella fastidiosa subsp. pauca (Xfp) in citrus plants. The siderophore production of Methylobacterium strains was tested according to chromeazurol agar assay test (CAS), Csáky test (hydroxamate-type) and Arnow test (catechol-type). In addition, the ability of Xfp to use siderophores, in vitro, produced by endophytic bacteria as source of iron, was evaluated. All 37 strains of Methylobacterium spp. tested were CAS-positive for siderophore production. Methylobacterium spp. produced hydroxamate-type, but not catechol-type siderophores. In vitro growth of Xfp was stimulated by the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus variegated chlorosis; Endophytes; Heterologous siderophores; Iron; Symbionts. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000400011 |
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| Azevedo,Fernando Alves de; Mourão Filho,Francisco de Assis Alves; Schinor,Evandro Henrique; Paoli,Luis Gustavo de; Mendes,Beatriz Madalena Januzzi; Harakava,Ricardo; Gabriel,Dean W.; Lee,Richard F.. |
| The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformation was accomplished by co-cultivation of tobacco and 'Valência' sweet orange explants with Agrobacterium tumefaciens containing the binary vector CsPP-GUS/2201. After plant transformation and regeneration, histochemical analyses using GUS staining revealed that CsPP promoter preferentially, but not exclusively, conferred gene expression in xylem tissues of tobacco. Weaker GUS staining was also detected throughout the petiole region in tobacco and citrus CsPP transgenic plants. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xylella fastidiosa; PAL; Citrus variegated chlorosis; Cultivar resistance. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001100008 |
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| Marucci,Rosangela Cristina; Giustolin,Teresinha Augusta; Miranda,Marcelo Pedreira de; Miquelote,Helen; Almeida,Rodrigo Piacentini Paes de; Lopes,João Roberto Spotti. |
| Rearing leafhopper (Hemiptera: Cicadellidae) vectors free of Xylella fastidiosa is a requirement for studies of various aspects of vector-pathogen interactions. The selection of a plant that allows vector development but not bacterial multiplication is desirable to produce healthy vectors. In this study, two leafhopper hosts, Vernonia condensata ('boldo') and Aloysia virgata ('lixeira') were needle inoculated with citrus and coffee strains of X. fastidiosa to evaluate if these plants support pathogen colonization. The inoculated plants did not present symptoms and the pathogen was not detected by culture and PCR tests, neither soon after inoculation (7-14 days) nor later, at 1, 4, 6 and 12 months after inoculation. To obtain healthy adults of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vernonia condensata; Citrus variegated chlorosis; Coffee leaf scorch; Sharpshooter vectors; Rearing technique. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400010 |
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| Gonçalves, Alline Maria Orbolato; UEM; Molina, Rúbia de Oliveira; UEM; Nunes, William Mário de Carvalho; UEM; Zanutto, Carlos Alexandre; UEM. |
| As cigarrinhas das famílias Cicadellidae e Cercopidae são vetores da bactéria Xylella fastidiosa Wells, causadora da Clorose variegada dos citros. Esta doença traz sérios prejuízos à lavoura de citros no Noroeste Paranaense, e é estudada no Brasil desde 1987, quando foi descoberta pela primeira vez na região de Colina, São Paulo. Os vetores transmitem a X. fastidiosa quando se alimentam sugando a seiva do xilema, local onde a bactéria se instala, obstruindo a passagem da seiva. Este trabalho teve o objetivo de avaliar a ocorrência das cigarrinhas vetoras Dilobopterus costalimai e Acrogonia citrina em pomar comercial de citros. O experimento foi realizado no sítio “Laranjeiras I”, município de Nova Esperança, região noroeste do Paraná, no período de julho... |
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Palavras-chave: 5.01.00.00-9 Agronomia Cicadellidae; Citrus sinensis; Xylella fastidiosa; Clorose Variegada dos Citros Fitossanidade Cicadellidae; Citrus sinensis; Xylella fastidiosa; Citrus variegated chlorosis. |
| Ano: 2008 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3502 |
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| Pacheco,Flávia Teresa Hansen; Silva-Stenico,Maria Estela; Etchegaray,Augusto; Gomes,José Elias; Carrilho,Emanuel; Tsai,Siu Mui. |
| Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane receptors, including siderophore, ferrichrome-iron and hemin receptors. All these biomolecules are thought to be associated with iron transport and utilization. Eighty isolates obtained from citrus orchards containing trees that developed citrus variegated chlorosis (CVC) were screened for siderophore production. The results demonstrated that only 10 of the isolates did not produce siderophores. Additional strains obtained from coffee, almond, mulberry, elm,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus variegated chlorosis; Plant pathogen; Iron-transportation; Pyoverdine; Enterobactin; Polyketide synthase; Peptide synthetase. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100026 |
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| Registros recuperados: 12 | |
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