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| Registros recuperados: 21 | |
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| Shirasuna, Koumei; Watanabe, S.; Oki, N.; Wijayagunawardane, M. P. B.; Matsui, Motozumi; Ohtani, M.; Miyamoto, Akio; 白砂, 孔明; 松井, 基純; 宮本, 明夫. |
| Prostaglandin F-2 alpha (PGF(2 alpha)) is the primary luteolysin in the cow, and luteal endothelin-1 (ET-1) interacts with PGF(2 alpha) during the process of luteolysis. In contrast, a developing corpus luteum (CL) is refractory to exogenous administration of PGF(2 alpha). Thus, the present study was aimed to investigate the functional relationship between ET-1 and PGF(2 alpha) in the mid-CL (PGF(2 alpha)-sensitive) and early-CL (PGF(2 alpha)-refractory). In the mid-CL model, cows (n = 6/treatment) were assigned to receive one of five types of treatments on day 10 of the estrous cycle: (1) an injection of saline; control, (2) a 500 jig of PGF2. analogue (sufficient dose to induce luteolytis); full-PG, (3) an intraluteal injection of 0.25 mg ET-1; ET-1, (4)... |
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Palavras-chave: Endothelin-1; Prostaglandin F-2 alpha; Luteolysis; Corpus luteum; Cow. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/825 |
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| Karimzadeh,L; Nabiuni,M; Sheikholeslami,A; Irian,S. |
| Polycystic ovarian syndrome (PCOS) is a low grade inflammatory disease characterized by hyperandrogenemia and chronic anovulation. C-reactive protein (CRP), released by adipocytes, plays a key role in PCOS. Apis mellifera honeybee venom (HBV) contains a variety of biologically active components with various pharmaceutical properties. This study was designed to assess the possibility of HBV application as an anti-inflammatory therapeutic agent. To induce PCOS, 1 mg/100 g body weight estradiol valerate (EV) was subcutaneously (SC) injected into eight-week-old rats. After 60 days, 0.5 mg/kg HBV was administered SC for 14 consecutive days, and the results of PCOS treatment were investigated. Rats were then anesthetized with chloroform, and their ovaries and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polycystic ovarian syndrome (PCOS); Honeybee venom (HBV); C-reactive protein (CRP); Estradiol valerate (EV); Corpus luteum; Chemiluminescence immunoassay. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400006 |
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| Stuhr,Bhárbara Delboni; Gil,Isaac Santos; Lacerda,Tracy Ferreira; Favoreto,Mauricio Gomes; Loureiro,Bárbara. |
| ABSTRACT: The equine chorionic gonadotropin (eCG) is a glycoprotein produced in mare endometrial calices. In bovine, it is used in estrus synchronization protocols. However, studies have shown that it is potentially immunogenic and its effect can decrease after repetitive use. This study aimed to evaluate antral follicle dynamics, corpus luteum (CL) and ovulation rate in bos indicus cows submitted to an estrus synchronization protocol in association with eCG for eight times consecutively. Ten cyclical, multiparous, and pasture raised beef cows were divided into two groups: control group (n=5) and eCG group (n=5). In 30 day interval, all animals were synchronized with the same estrogen/progesterone based protocol, totalizing 8 re-synchronizations. Cows in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Corpus luteum; ECG; Fertility; Immunity; FTAI. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001102018 |
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| SHIRASUNA, Koumei; ASAHI, Takayuki; SASAKI, Motoki; SHIMIZU, Takashi; MIYAMOTO, Akio; 白砂, 孔明; 佐々木, 基樹; 清水, 隆; 宮本, 明夫. |
| We have shown that luteal blood flow increases in the peripheral vasculature of the mature corpus luteum (CL) prior to the onset of luteolysis in response to prostaglandin F2α (PGF2α) in the cow, but this phenomenon does not occur in the early CL. We therefore hypothesize that this acute increase of luteal blood flow occurs by vasodilation of large blood vessels due to local release of nitric oxide (NO). In the present study, we characterized the CL vasculature together with endothelial NO synthase (eNOS) expression during the estrous cycle in the cow. Immunohistochemistry was used to quantify the number of arteriolovenous vessels (surrounded with smooth muscle actin-positive smooth muscle cells), capillaries (with von Willebrand Factor-positive... |
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Palavras-chave: Blood vessels; Corpus luteum; ENOS; Luteolysis. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2989 |
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| Watanabe, Sho; Shirasuna, Koumei; Matsui, Motozumi; Yamamoto, Dai; Berisha, Bajram; Schams, Dieter; Miyamoto, Akio; 白砂, 孔明; 松井, 基純; 宮本, 明夫. |
| Endothelin-1 (ET-1) is a luteolytic mediator in the bovine corpus luteum (CL), and its action appears to be via endothelin type A receptor (ETR-A). Thus, the aim of the present study was to determine the effect of ETR-A antagonist on PGF(2)alpha-induced luteolysis in the cow. Cows on days 1012 of the estrous cycle were subjected to five intraluteal injections of the ETR-A antagonist LU 135252 in saline or only saline at -0.5, 2, 4, 6, and 8 h after PGF(2)alpha administration (=0 h). Serial luteal biopsies were conducted to determine the expression of mRNA in the luteal tissue. There were no significant differences in the decrease in plasma progesterone (P) concentrations and the mRNA expressions of steroidogenic acute regulatory protein and 3... |
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Palavras-chave: Apoptosis; Blood flow; Corpus luteum; Cow; Endothelin-1. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/922 |
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| Alves,Nadja Gomes; Torres,Ciro Alexandre Alves; Guimarães,José Domingos; Moraes,Elenice Andrade; Rodrigues,Marcelo Teixeira; Cecon,Paulo Roberto; Bitencourt,Luciene Lignani; Amorim,Lincoln da Silva. |
| To evaluate the effect of using urea in the diet on ovarian follicular dynamics and plasma urea and progesterone concentration, an experiment was carried out with 29 Alpine goats randomly distributed into four diets, each one containing 0%; 0.73%; 1.46% or 2.24% of urea on total dry matter. The follicular dynamics was monitored daily by ultrasonography. The estrous cycles were characterized by occurrence of two, three, four or five follicular waves. The emergence day of the first follicular wave in cycles with two waves, the length of the third wave and the maximum diameter of the largest follicle of the third wave in cycles with three waves presented a quadratic behavior according to the percentage of urea in the diets. In cycles with four waves, the urea... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Corpus luteum; Estrous cycle; Follicular development; Protein. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000700016 |
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| SHIRASUNA, Koumei; WATANABE, Sho; NAGAI, Kaya; SASAHARA, Kiemi; SHIMIZU, Takashi; RICKEN, Albert M.; SPANEL-BOROWSKI, Katharina; MIYAMOTO, Akio; 白砂, 孔明; 清水, 隆; 宮本, 明夫. |
| Cell-to-cell interaction via cell contact-dependent pathway is essentially important for maintenance and regulation of corpus luteum (CL) integrity and its physiological actions. The objective of the present study was to evaluate the mRNA expression of the cell adhesion molecules (CAMs) that are constituent factors of gap junctions [connexin (Cx) 43] and adherence junctions (VE-, E-, N-cadherin) in two types of endothelial cells from the mid CL and in CL tissue during the estrous cycle and PGF2α-induced luteolysis in the cow. Specific mRNA expression for Cx43 and N-cadherin was detected in cytokeratin-positive (CK+) and cytokeratin-negative (CK-) luteal endothelial cells (EC) and fully luteinized granulosa cells (LGC). E-cadherin mRNA was expressed in... |
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Palavras-chave: Cadherin; Connexin; Corpus luteum; Cow; Luteolysis. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2988 |
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| Xavier,P.R.; Leão,R.A.C.; Oliveira e Silva,P.V.D.; Marques Júnior,A.P.. |
| Foram avaliadas características morfológicas do corpo lúteo de 48 vacas Nelore gestantes obtidos de abatedouros. Os ovários com o corpo lúteo foram coletados, identificados e divididos em três grupos, considerando o estágio da gestação determinado pelo tamanho do feto: Grupo I - onze animais com gestação até 90 dias; Grupo 2 - vinte animais com gestação de 90 a 180 dias, e Grupo 3 - 17 animais com gestação de 180 a 261 dias. Todos os corpos lúteos foram dissecados, submetidos a processamento histológico e avaliados utilizando microscopia de luz. As características morfológicas das células luteais esteroidogênicas não mudou durante a gestação. Porém, foi observado um aumento de tecido conjuntivo, fibroblastos e matriz extracelular durante o final da... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nelore cow; Pregnancy; Corpus luteum; Secretion granules. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200036 |
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| Arashiro,Eduardo Kenji Nunes; Viana,João Henrique Moreira; Fonseca,Jeferson Ferreira da; Camargo,Luiz Sérgio de Almeida; Fernandes,Carlos Antônio de Carvalho; Brandão,Felipe Zandonadi. |
| The aim of this study was to establish the morphologic and endocrine characteristics of luteal dynamics in goats. It was used Toggenburg female goats that showed natural estrus in a 48-hour interval. After estrus, ultrasonographic evaluations of the ovaries were daily performed during 21 days using a portable device (5MHz probe). Blood sample was collected for plasma progresterone (P4) determination. Corpora lutea were detected for the first time on day 5 and progressively increased in size until D9 (1.26 ± 0.08 cm²), with no variation on subsequent days. In females with one ovulation, the first visualization of the corpora lutea was earlier than in those with multiple ovulation (4.54 ± 0.18 vs 5.74 ± 0.25 days). At the moment of the first visualization,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Corpus luteum; Goats; Progesterone; Ultrasonography. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000900011 |
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| Alves,N.G.; Torres,C.A.A.; Guimarães,J.D.; Moraes,E.A.; Costa,P.B.; Silva,D.R.. |
| RESUMO Os objetivos deste estudo foram relatar a dinâmica folicular ovariana e a concentração plasmática de progesterona durante ciclos estrais de duração irregular em cabras da raça Alpina. Vinte e nove cabras tiveram sua atividade ovariana monitorada diariamente por ultrassonografia transretal durante um, dois ou três períodos interovulatórios consecutivos. Amostras de sangue foram coletadas para análise da concentração plasmática de progesterona. Dos 47 ciclos avaliados, 28 (59,57%) foram de duração normal (17 a 25 dias), sete (14,89%) de curta duração (<17 dias) e 12 (25,53%) de longa duração (>25 dias). Os ciclos estrais de curta duração tiveram 6,14±0,69 dias e uma onda folicular que emergiu no dia 0,43±1,13. Os ciclos estrais de longa duração... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Follicular development; Corpus luteum; Progesterone; Short estrous cycles. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000602017 |
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| MIURA, Ryotaro; HANEDA, Shingo; MATSUI, Motozumi; 羽田, 真悟; 松井, 基純. |
| The objective of our study was to compare the characteristics of the corpus luteum (CL) formed after ovulation of the dominant follicle (DF) of the first follicular wave (W1) and those of the CL formed after ovulation of the DF of the second (induced) follicular wave (W2). Non-lactating Holstein cows were used for this study. In Experiment 1, cows were treated with PGF2α and GnRH on days 6 and 8 (day 0 = day of follicular wave emergence) for W1 (n = 6) and W2 (n = 6), respectively. Dominant follicles were aspirated on day 9 to quantify the amounts of mRNA (VEGF120, VEGF164, FGF-2, StAR, P450-scc and 3β-HSD) in granulosa cells (GC). In Experiment 2, the size and blood flow area of the CL formed after ovulation of the DF in W1 (W1CL; n = 6) and W2 (W2CL; n =... |
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Palavras-chave: Blood flow; Corpus luteum; Cow; Follicular wave; Gene expression. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4147 |
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| SHIRASUNA, Koumei; SASAHARA, Kiemi; MATSUI, Motozumi; SHIMIZU, Takashi; MIYAMOTO, Akio; 白砂, 孔明; 松井, 基純; 清水, 隆; 宮本, 明夫. |
| Administration of prostaglandin (PG) F2α in cattle during the mid-luteal phase (Days 8-12 of the estrous cycle) drastically reduces the plasma progesterone concentrations and the volume of the corpus luteum (CL). However, PGF2α does not induce luteolysis during the early luteal phase (up to Day 5 of the estrous cycle). To characterize the possible distinct difference in acute response to a luteolytic dose of PGF2α administration, we determined various mRNA expressions in the early and mid CL relating to angiogenesis, vasoactivation and PG-related factors at 30 min after PGF2α injection in cyclic cows. The experiments were conducted on Day 4 (early CL) and Days 10-12 (mid CL). Cows were either injected with 500 μg PGF2α analogue or saline as the control... |
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Palavras-chave: Corpus luteum; Cow; Luteolysis; Prostaglandin F2α. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2812 |
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| TANAKA, Jun; ACOSTA, Tomas J.; BERISHA, Bajram; TETSUKA, Masa; MATSUI, Motozumi; KOBAYASHI, Shuichi; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫. |
| Local angiogenesis and angiolysis in the corpus luteum (CL) relate to the luteal function. Recent studies indicate that angiopoietins (ANPT) and their receptors Tie regulate remodeling of microvasculature. We therefore examined 1) the relative changes in the expression of mRNA for ANPT-1, ANPT-2, Tie1 and Tie2 in bovine CL by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) during the estrous cycle and prostaglandin F2α (PGF2α)-induced luteolysis, and 2) the effect of ANPT-2 on progesterone (P4) release from CL at the late stage of the estrous cycle by an in vitro microdialysis system (MDS). The CLs were classified into 4 stages (early: Day 2-5, n=7, mid: Day 8-12, n=15, late: Day 15-17, n=9, regressing: Day >18, n=19). The... |
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Palavras-chave: Angiopoietin; Angiolysis; Luteolysis; Corpus luteum; Cow. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2977 |
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| BEINDORFF, Nicola; NAGAI, Kaya; SHIRASUNA, Koumei; HERZOG, Kathrin; HOEFFMANN, Katharina; SASAKI, Motoki; BOLLWEIN, Heinrich; MIYAMOTO, Akio; 佐々木, 基樹; 宮本, 明夫; 白砂, 孔明. |
| The present study determined vascular changes in the bovine corpus luteum (CL) at Day 16 (early maternal recognition period) and Day 40 in early pregnancy and compared them to the CL from Day 12 and Day 16 of the estrous cycle. The CLs were analyzed in the central and peripheral regions, where site-depending features of vessels and angiogenic factors are evident. The same protein level of the endothelial cell marker von Willebrand factor was retained in the CL from Day 16 of the estrous cycle to Day 40 of early pregnancy. The protein level of pericytes and smooth muscle cells was determined using smooth muscle α-actin; the level decreased at Day 40 of early pregnancy in both regions of the CL. No significant change in the expressions of vascular... |
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Palavras-chave: Corpus luteum; Cow; Early pregnancy; Endothelial cells. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2811 |
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| Registros recuperados: 21 | |
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