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| Florindo,M.I.; Aragão,M.E.F. de; Silva,A.C.M. da; Otoch,M.L.; Melo,D. Fernandes de; Lima,J.A.A.; Lima,M.G. Silva. |
| There is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. Cowpea severe mosaic virus (CPSMV), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (Vigna unguiculata) leaves infected with CPSMV both administered by gavage to Swiss mice induced a humoral immune response. Groups of 10 Swiss mice (2-month-old females) were immunized orally with 10 daily doses of either 50 µg viral capsid protein (boosters of 50 µg at days 21 and 35 after immunization) or 0.6 mg protein of the crude extract (boosters of 0.6 mg at days 21 and 35 after immunization). Anti-CPSMV antibodies were quantified by ELISA in pooled sera... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oral immunization; Plant virus; Plant virus immunogenicity; Cowpea severe mosaic virus. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700011 |
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| Lima,José Albersio Araujo; Silva,Ana Kelly Firmino da; Aragão,Maria do Livramento; Ferreira,Nádia Rutielly de Araújo; Teófilo,Elizita Maria. |
| The objective of this work was to identify new sources of simple and multiple resistances to Cowpea severe mosaic virus (CPSMV), Cowpea aphid-borne mosaic virus (CABMV) and Cucumber mosaic virus (CMV) isolates in cowpea (Vigna unguiculata). Thirty-three genotypes from the germplasm bank of Universidade Federal do Ceará were tested as to their resistance to four CPSMV isolates, two CABMV isolates and one CMV isolate. Twenty-five days after the first virus inoculations, all inoculated plants, including the asymptomatic ones, were tested by serology. Genotypes were classified as: immune, plants without symptoms and negative serology; resistant, plants with mild mosaic and positive serology; susceptible, plants with mosaic and positive serology; and highly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cowpea aphid-borne mosaic virus; Cowpea severe mosaic virus; Cucumber mosaic virus; Vigna unguiculata; Serology; Virus control. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001100003 |
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