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| Pedroso,Reginaldo dos Santos; Ferreira,Joseane Cristina; Costa,Karen Regina Carim da; Candido,Regina Celia. |
| Various organisms have been characterized by molecular methods, including fungi of the genus Cryptococcus. The purposes of this study were: to determine the discriminatory potential of the RAPD (Random Amplified Polymorphic DNA) primers, the pattern of similarity of the Cryptococcus species, and discuss their useful application in epidemiological studies. We analyzed 10 isolates of each specie/group: C. albidus, C. laurentii complex, C. neoformans var. grubii, all from environmental source, and two ATCC strains, C. neoformans var. grubii ATCC 90112, and C. neoformans var. neoformans ATCC 28957 by RAPD-PCR using the primers CAV1, CAV2, ZAP19, ZAP20, OPB11 and SEQ6. The primers showed a good discriminatory power, revealing important differences between them... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptococcus albidus; Cryptococcus laurentii; RAPD; Molecular Markers. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300015 |
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| Castanha,Rodrigo Fernandes; Mariano,Adriano Pinto; Morais,Lilia Aparecida Salgado de; Scramin,Shirlei; Monteiro,Regina Teresa Rosim. |
| This study aimed the optimization of culture condition and composition for production of Cryptococcus laurentii 11 biomass and lipids in cheese whey medium supplemented with sugarcane molasses. The optimization of pH, fermentation time, and molasses concentration according to a full factorial statistical experimental design was followed by a Plackett-Burman experimental design, which was used to determine whether the supplementation of the culture medium by yeast extract and inorganic salts could provide a further enhancement of lipids production. The following conditions and composition of the culture medium were found to optimize biomass and lipids production: 360 h fermentation, 6.5 pH and supplementation of (g L-1): 50 molasses, 0.5 yeast extract, 4... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptococcus laurentii; Microbial lipids; Cheese whey; Sugarcane molasses; Biodiesel. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200003 |
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