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Registros recuperados: 24
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Active human Cytomegalovirus infection and Glycoprotein B genotypes in Brazilian pediatric renal or hematopoietic stem cell transplantation patients BJM
Dieamant,Débora de Campos; Bonon,Sandra Helena Alves; Prates,Liliane Cury; Belangelo,Vera Maria Santoro; Pontes,Erika R.; Costa,Sandra Cecília Botelho.
A prospective analysis of active Human Cytomegalovirus infection (HCMV) was conducted on 33 pediatric renal or hematopoietic stem cell post-transplant patients. The HCMV-DNA positive samples were evaluated for the prevalence of different gB subtypes and their subsequent correlation with clinical signs. The surveillance of HCMV active infection was based on the monitoring of antigenemia (AGM) and on a nested polymerase chain reaction (N-PCR) for the detection of HCMV in the patients studied. Using restriction analysis of the gB gene sequence by PCR-RFLP (Restriction Fragment Length Polymorphism), different HCMV strains could be detected and classified in at least four HCMV genotypes. Thirty-three pediatric recipients of renal or bone marrow transplantation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytomegalovirus; Pediatric transplantation; GB genotypes; PCR; Antigenemia.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100010
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Alemtuzumab as graft-versus-host disease (GVHD) prophylaxis strategy in a developing country: lower rate of acute GVHD, increased risk of cytomegalovirus reactivation BJMBR
Resende,C.B.; Rezende,B.M.; Bernardes,P.T.T.; Teixeira,G.M.; Teixeira,M.M.; Pinho,V.; Bittencourt,H..
Acute graft-versus-host disease (aGVHD) and cytomegalovirus reactivation are important complications after allogeneic stem cell transplantation (alloHSCT). Here, we evaluated the impact of treatment with alemtuzumab on the occurrence of aGVHD, cytomegalovirus reactivation and survival after alloHSCT. This was a prospective cohort study conducted at the allo-HSCT unit of Hospital das Clínicas, Universidade Federal de Minas Gerais, Brazil, from January 2009 to December 2011. Fifty-seven patients who underwent alloHSCT were included. Forty-five (79%) patients had a malignant disease. Alemtuzumab was administered before the conditioning regimen at a dose of 1 mg/kg in children and 30 mg/day for 2 days in adults or children weighing more than 40 kg (a total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hematopoietic stem cell transplantation; Alemtuzumab; Graft-versus-host disease; Cytomegalovirus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200705
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Clinical correlates of pp65 antigenemia monitoring in the first months of post kidney transplant in patients undergoing universal prophylaxis or preemptive therapy BJID
Carvalho,Fabiana Rabe; Cosendey,Rachel Ingrid Juliboni; Souza,Cintia Fernandes; Medeiros,Thalia; Menezes,Paulo Alexandre; Silva,Andrea Alice; Almeida,Jorge Reis; Lugon,Jocemir Ronaldo.
Abstract Introduction: Human cytomegalovirus is a major cause of morbidity in kidney transplant patients. Objectives: We aimed to study viral replication and serological response in the first months post kidney transplant in patients undergoing universal prophylaxis or preemptive therapy and correlate the findings with the clinical course of Human cytomegalovirus infection. Patients and methods: Independent from the clinical strategy adopted for managing Human cytomegalovirus infection, prophylaxis versus preemptive therapy, the pp65 antigenemia assay and serological response were assessed on the day of transplantation, and then weekly during the first three months of post-transplant. Results: From the 32 transplant recipients, 16 were positive for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytomegalovirus; Pp65 antigenemia; Kidney transplant; Universal prophylaxis; Preemptive therapy.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100051
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Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients BJID
Carraro,Emerson; Pasternak,Jacyr; Perosa,Ana; Siqueira,Itacy; Martino,Marines Dalla Vale.
Cytomegalovirus (CMV) antigenemia is still one of the two major assays available for diagnosis and monitoring of CMV infections. A commercial rapid test recently available in Brazil for quantification of human cytomegalovirus pp65 antigenemia revealed by immunofluorescence technique was compared with the original in-house method revealed by immunoperoxidase in patients receiving solid organ transplants. Of 80 blood samples tested for CMV antigenemia, 34 (42.5%) were positive: commercial assay detected 33 (97%) and in-house assay detected 20 (58.8%) samples. The numbers of positive cells in the two assays were different, with a median of 4.5 and 12 positive cells obtained by in-house and commercial kit, respectively. Discrepancies between assays occurred in...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cytomegalovirus; Antigenemia assay; Immmunoperoxidase; Immunofluorescence.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300023
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Comparison of immunoperoxidase and immunofluorescence assays for pp65 cytomegalovirus antigen in immunocompromised patients BJID
Debur,Maria do Carmo; Vidal,Luine R. Rosele; Nogueira,Meri Bordignon; Pereira,Luciane Aparecida; Takahashi,Gislene R.A.; Rotta,Indianara; Almeida,Sérgio Monteiro de; Raboni,Sonia Mara.
We compared the pp65 antigen detection by an in house method (immunoperoxidase assay) and by a commercial kit (immunofluorescence assay) available for cytomegalovirus infection diagnosis in immunocompromised patients. Sixty-four blood samples were analyzed in duplicate for both techniques. Eight-six percent of the samples had concordant qualitative results. The discordant results occurred more frequently in samples with low quantity of positive cells. There were no significant differences with qualitative and quantitative results of the methods.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunoperoxidase; Immunofluorescence; Cytomegalovirus; Immunocompromised patients; Bone marrow transplantation; Solid organ transplant.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200014
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Cytomegalovirus and Toxoplasma gondii seroprevalence in a Brazilian liver transplant waiting J. Venom. Anim. Toxins incl. Trop. Dis.
Almeida,R. A. M. B.; Silva,G. F.; Llanos,J. C.; Winckler,C. C.; Gomes,M. R. B.; Biagioni,D. S.; Paula,R. C. C.; Meira,D. A.; Bakonyi,A..
Cytomegalovirus (CMV) disease is a major cause of morbidity and mortality in solid organ transplantation. Disseminated toxoplasmosis after liver transplantation is a rare but fatal event. Serologic screening of the donor and the recipient is essential to prophylactic management, early diagnosis and therapeutic strategies to minimize the consequences of these infections. The aim of the present study was to determine the seroprevalence of CMV and Toxoplasma gondii (TG) in a Brazilian liver transplant waiting list (LTWL). Serological data were collected from 44 candidates on the LTWL between May 2003 and November 2004. Serological investigation of antibodies IgM and IgG against CMV (anti-CMV) and TG (anti-T. gondii) was performed using fluorometry commercial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytomegalovirus; Liver transplantation; Toxoplasmosis; Toxoplasma gondii; Seroprevalence; Waiting list.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400016
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Cytomegalovirus (CMV)-related cutaneous necrotizing vasculitis: case report and literature review BJID
Arslan,Ferhat; Batirel,Ayse; Mert,Ali; Ozer,Serdar.
Cytomegalovirus (CMV) infection is usually asymptomatic in immunocompetent patients. A mononucleosis-like syndrome may develop in some patients. Various organ involvements (eg: encephalitis, meningitis, retinitis, myocarditis, pneumonia, hepatitis, enterocolitis, neuritis), which rarely occur in immunocompetent patients, have also been reported. Cutaneous necrotizing vasculitis caused by CMV infection has been reported very rarely in the literature. Here, a case with a very rare clinical form of CMV infection, presenting with persistent fever and livedo reticularis on the extremities and cutaneous necrotizing vasculitis of the toes, is described, and the relevant literature is reviewed. This case report aims to highlight the possibility of CMV infection to...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Cytomegalovirus; Vasculitis; Cutaneous.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500015
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Cytomegalovirus infection in renal transplant recipients diagnosed by nested-PCR BJMBR
Aquino,V.H.; Figueiredo,L.T.M..
A prospective study of cytomegalovirus (CMV) infection was carried out on 34 renal transplant recipients managed at a General Hospital in Ribeirão Preto, SP, Brazil. Serologic tests showed that all patients were infected with CMV before renal transplantation. Two nested-PCR techniques with primers that recognize sequences of the glycoprotein B (gB) and H (gH) genes were used for CMV detection in blood and urine samples during the post-transplantation period. CMV was detected more frequently in blood samples than in urine samples (P<0.001). Thirty-three patients had CMV detected at least once in blood and/or urine samples. Seven of these patients (21.2%) were diagnosed as having symptomatic CMV infection and showed a worse clinical outcome, with a higher...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytomegalovirus; Polymerase chain reaction; Kidney transplantation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100011
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Detection of human cytomegalovirus and Epstein-Barr virus in coronary atherosclerotic tissue BJM
Imbronito,Ana Vitória; Marcelino,Silvia Linardi; Grande,Sabrina Rosa; Nunes,Fabio Daumas; Romito,Giuseppe Alexandre.
Previous studies indicated that patients with atherosclerosis are predominantly infected by human cytomegalovirus (HCMV), but rarely infected by type 1 Epstein-Barr virus (EBV-1). In this study, atheromas of 30 patients who underwent aortocoronary bypass surgery with coronary endartherectomy were tested for the presence of these two viruses. HCMV occurred in 93.3% of the samples and EBV-1 was present in 50% of them. Concurrent presence of both pathogens was detected in 43.3% of the samples.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atherosclerosis; Cytomegalovirus; Epstein-Barr virus.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300004
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Does mycophenolate mofetil increase the risk of cytomegalovirus infection in solid organ transplant recipients?: A mini-review BJID
Song,Alice Tung Wan; Abdala,Edson; Bonazzi,Patrícia Rodrigues; Bacchella,Telésforo; Machado,Marcel Cerqueira César.
Mycophenolate mofetil (MMF) is currently used for prophylaxis of acute rejection in solid organ transplantation. There have been diverging reports regarding an association between MMF and the risk of cytomegalovirus (CMV) infection. We reviewed the main published studies in an attempt to clarify the association between the use of MMF and the risk, frequency and severity of CMV infections. In a search of the Medline database with the terms "mycophenolate" and "cytomegalovir*", 42 articles were found to be relevant; among these, 29 articles were thoroughly analyzed. The first studies on MMF in renal transplantation already showed a tendency towards an association between this drug and the occurrence of CMV disease. Further studies were designed specifically...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycophenolate mofetil; Cytomegalovirus; Transplant.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000200011
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Fulminant pancytopenia due to cytomegalovirus infection in an immunocompetent adult BJID
Koukoulaki,Maria; Ifanti,Georgia; Grispou,Eirini; Papastamopoulos,Vassilios; Chroni,Georgia; Diamantopoulos,Emmanouil; Skoutelis,Athanasios.
A case of severe and irreversible pancytopenia secondary to acute primary cytomegalovirus infection in an immunocompetent woman is described. The patient presented with thrombocytopenia, lymphopenia, anemia, and abnormal liver function tests. Treatment with corticosteroids and intravenous immunoglobulin was ineffective in reconstituting hemopoiesis. The patient developed severe sepsis and eventually expired.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Cytomegalovirus; Pancytopenia; Immunocompetent; Fulminant; Irreversible.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200012
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HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil BJM
Spano,Liliana Cruz; Ferreira,Mônica Simões Rocha; Almeida,Marilda Santos; Nascimento,Jussara Pereira do; Leite,José Paulo Gagliardi.
Human cytomegalovirus (HCMV) displays genetic variability in several regions, supposed to be related with strain-specific tissue tropism and immunopathogenesis. Based on sequence variation in the UL55 gene that encodes gB glycoprotein, HCMV strains can be assigned to one of four genotypes. Previous studies have addressed gB genotyping mostly by investigating strains derived from immunosuppressed patients, sometimes without previous knowledge about genotype distribution in a geographic area. The present study verified the distribution of HCMV gB genotypes of strains obtained from immunocompetent women at Vitória City, Espírito Santo State, Southeastern, Brazil. The HCMV genome was extracted from their cervical secretion, fetal and maternal placenta tissues...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytomegalovirus; RFLP; GB genotypes; Cervical secretion; Placenta tissues.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300008
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Human herpesvirus-6 and cytomegalovirus DNA in liver donor biopsies and their correlation with HLA matches and acute cellular rejection BJID
Guardia,Ana Carolina; Stucchi,Raquel Silveira Bello; Milan,Arlete; Costa,Sandra Cecília Botelho; Boin,Ilka de Fátima Santana Ferreira.
Herpesvirus reactivation is common after liver transplantation.OBJECTIVE:Analyze the presence of cytomegalovirus (HCMV) and human herpesvirus-6 (HHV-6) DNA in liver donor biopsies, seeking to better understand issues involving human donor leukocyte antigens (HLA)-A, B and DR, as well as correlations with acute cellular rejection.METHODS:Fifty-nine liver transplantation patients were investigated for the presence of HCMV and HHV-6 DNA in liver donor biopsies, using the Nested-PCR technique. The clinical donor information and HLA matches were obtained from the São Paulo State Transplant System. The recipients' records regarding acute cellular rejection were studied.RESULTS:Seven (11.8%) biopsies were positive for HCMV DNA and 29 (49%) were positive for HHV-6...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Liver transplantation; Human herpesvirus 6; Cytomegalovirus; Liver donor biopsies.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200220
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ISOLATION OF THE HUMAN CYTOMEGALOVIRUS FROM BODILY FLUIDS Acta biol.Colomb.
CAMACHO ORTEGA,Sigrid Johanna; BOHORQUEZ ÁVILA,Sonia del Pilar; VELANDIA ROMERO,Myriam Lucia; CASTELLANOS PARRA,Jaime Eduardo.
ABSTRACT In vitro studies on the pathogenesis of the human cytomegalovirus (HCMV) are conducted regularly using laboratory adapted strains that lose some characteristics during the adaptation process. Since HCMV is excreted from bodily fluids during infection or reactivation, this work aimed to isolate and culture HCMV from the MRC-5 human cells found in the urine, bronchoalveolar lavage, saliva, and plasma samples of pediatric patients with probable or confirmed infection. The samples were inoculated on cell cultures either for 14 days or until a cytopathic effect (CPE) of 80 % was observed. The cell lysates and supernatants were used to perform successive viral passages. Besides HCMV, the herpes simplex virus was detected from all the saliva samples....
Tipo: Journal article Palavras-chave: Body fluids; Cell culture; Cytomegalovirus; Isolation.
Ano: 2019 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300520
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Lymphocyte subpopulations during cytomegalovirus disease in renal transplant recipients BJMBR
Castro,S.M.; Sporleder,H.; Schröeder,R.; Santos,A.; Garcia,V.; Neumann,J.; Costa,S.C.B..
We have determined the number of circulating T, B and natural killer cells in renal transplant recipients in order to detect changes during cytomegalovirus (CMV) infections. Serial blood samples were taken from 61 patients on standard triple immunosuppression therapy (cyclosporin A, azathioprine and prednisone). Using two-color flow cytometry analysis, the absolute number of CD3+, CD4+, CD8+, CD19+, CD3+HLA-DR+ and CD16+56+ cells was determined. Forty-eight patients (78.7%) developed active CMV infection, and all of them subsequently recovered. Twenty of the infected patients (32.8%) presented symptoms compatible with CMV disease during the infectious process. The number of lymphocytes and their main subpopulations were normal before the onset of CMV...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transplantation; Cytomegalovirus; Antigenemia; T lymphocytes.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600016
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Molecular characterization of viruses associated with gastrointestinal infection in HIV-positive patients BJID
Silva,Raquel C; Benati,Fabrício J; Pena,Giselle PA; Santos,Norma.
BACKGROUND: Diarrhea is a major cause of morbidity and mortality among HIV-infected patients worldwide. OBJECTIVE: We sought to determine the frequency of viral gastrointestinal infections among Brazilian HIV-infected patients with diarrhea. METHODS: A collection of 90 fecal specimens from HIV-infected individuals with diarrhea, previously tested for the presence of bacteria and parasite was analyzed by polymerase chain reaction and sequence analysis for the presence of enteric viruses such as astrovirus, norovirus, rotavirus groups A, B and C, adenovirus, herpes simplex virus, Epstein-Barr virus, cytomegalovirus, and human bocavirus. RESULTS: Twenty patients (22.2%; n = 90) were infected with parasites (11 single infections and nine coinfected with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV infection; Gastrointestinal disorders; Cytomegalovirus; Human bocavirus.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600001
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Multiorgan involvement due to cytomegalovirus infection in AIDS BJID
Majumder,Shounak; Mandal,Sanjay K.; Bandyopadhyay,Dipanjan; Chowdhury,Subhasis Roy; Chakraborty,Partha P.; K.,Mitra.
Cytomegalovirus (CMV) infection is a relatively late complication of AIDS. Like other viruses contributing to co-morbidity of HIV infection, cytomegalovirus has the propensity to cause multiorgan involvement. We report the case of a 34-year-old seropositive man who presented with bilateral lower limb weakness and symptomatic pallor. He was already on antiretroviral drugs for a month prior to presentation. Detailed clinical examination and laboratory investigations revealed cytomegalovirus polyradiculoneuropathy associated with bone marrow dysplasia. Dysplasia of haematopoeitic cell lines occurs in 30% to 70% of HIV infected patients, and is often indistinguishable from myelodysplastic syndrome. However, in our case, the bone marrow picture reverted back to...
Tipo: Info:eu-repo/semantics/report Palavras-chave: AIDS; Cytomegalovirus; Multiorgan involvement.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100039
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PP65 antigenemia in the diagnosis of cytomegalovirus infection in AIDS patients J. Venom. Anim. Toxins incl. Trop. Dis.
Capela,RC; Grassi,A; Souza,LR.
Cytomegalovirus causes significant morbidity and mortality in AIDS patients and those having undergone bone marrow or another transplant. PP65 antigenemia is based on detecting viral antigen in peripheral blood leukocytes through immunochemistry and by monitoring the infection in immunocompromised individuals. The present study aimed to set up this diagnostic technique in AIDS patients with active cytomegalovirus infection and verify its occurrence in the Botucatu region of São Paulo state, Brazil. Fifty patients, 35 men and 15 women aged from 24 to 69 years, were recruited from those attended at the Department of Tropical Diseases of Botucatu Medical School, UNESP, and divided into three groups according to CD4+ T lymphocyte counts and antiretroviral...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acquired immunodeficiency syndrome; Cytomegalovirus; Pp65 antigen; Laboratory diagnosis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100013
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Prognostic markers of symptomatic congenital cytomegalovirus infection BJID
Romanelli,Roberta Maia de Castro; Magny,Jean François; Jacquemard,François.
The objective of this research was to identify maternal and fetal characteristics as prognostic markers of congenital cytomegalovirus (CMV) infection. This is a descriptive study of 13 cases of congenital CMV infection referred to Institute de Puericulture et Perinatologie de Paris (IPP) from January 2005 to October 2006. Amniotic fluid puncture was performed to research CMV polimerase chain reaction (PCR). Cordocentesis and cord blood samples at delivery were also analyzed to determinate fetal platelets count, GGT, ASAT, ALAT, CMV-DNA and IgM antibody. Variables of symptomatic and asymptomatic infants were then compared. Data were analyzed by SPSS - 15.0. Mean gestational age of amniocentesis was 24.6 weeks and there was no difference of mean viral load...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytomegalovirus; Disease transmission; Vertical; Prenatal diagnosis; Prognosis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100009
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Receptor expression and responsiveness of human peripheral blood mononuclear cells to a human cytomegalovirus encoded CC chemokine BJID
Zheng,Qi; Xu,Jun; Gao,Huihui; Tao,Ran; Li,Wei; Shang,Shiqiang; Gu,Weizhong.
Human cytomegalovirus is a ubiquitous pathogen that infects the majority of the world's population. After long period of time co-evolving with human being, this pathogen has developed several strategies to evade host immune surveillance. One of the major trick is encoding homologous to those of the host organism or stealing host cellular genes that have significant functions in immune system. To date, we have found several viral immune analogous which include G protein coupled receptor, class I major histocompatibility complex and chemokine. Chemokine is a small group of molecules which is defined by the presence of four cysteines in highly conserved region. The four kinds of chemokines (C, CC, CXC, and CX3C) are classified based on the arrangement of 1 or 2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chemokine; Cytomegalovirus; Receptor; Immune.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400403
Registros recuperados: 24
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