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Sandoval,Catherine; Nuñez,Marcelo; Roa,Ignacio. |
The need to identify bodies that are found as a result of disappearances with a diversity of causes, illegal burials and massive disasters, represent a wide percentage of dentistry practice on forensic research. The following study determined the performance of Barr Body Test, in fibroblasts of healthy teeth, under different conditions of burial (in vitro) with variations in pH, humidity and salinity in terms of general accuracy and sensitivity for men and women. Analyzed sample considered 47 dental pulps, taken from teeth under burial conditions during a period of a month. From dental pulps samples, 265 histological cuts valid for this study, were obtained, which were observed with an optical microscope under conventional H/E staining. Results showed a... |
Tipo: Journal article |
Palavras-chave: Barr body; Fibroblast; Dental pulp; Sex identification; Burial conditions. |
Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200026 |
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Bertassoli,Bruno Machado; Costa,Emanuela Silva; Sousa,Cristiane Aparecida; Albergaria,Juliano Douglas Silva; Maltos,Kátia L. Melo; Goes,Alfredo Miranda; Matins,Thais Maria da Mata; Silva,Gerluza Aparecida Borges; Jorge,Erika Cristina. |
ABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHCI, CD54, CD73, CD90, CD45, CD11 and CD34); RT-PCR for Nanog gene; and their differentiation capacity in osteogenic, adipogenic and chondrogenic cell lines. Isolated cells exhibited adhesion capacity to plastic; fusiform morphology, and 80% confluence reached in approximately 3 days. These cells have also revealed positive expression for CD54, CD73 and CD90 markers; and negative expression for CD11, CD34... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mesenchymal stem cells; Dental pulp; Murine; Phenotypic characterization; Cellular differentiation. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335 |
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